Evaluation of the in vitro and in vivo anticancer properties of Moroccan propolis extracts

Abstract

This investigation aimed to evaluate the in vitro and in vivo antitumor potential of a Moroccan propolis extracts. For in vitro assays, three mammalian tumor cell lines were used: BSR (hamster renal adenocarcinoma), Hep-2 (human laryngeal carcinoma) and P815 (murin mastocytoma). The propolis ethanolic extract as well as the ethyl acetate extract, exert an in vitro cytotoxic activity in dose-dependent manner. The IC50 values were ranging from 15 µg/mL to 38 µg/mL. This activity depends not only on the extract's chemical composition (analysed by HPLC/ESI-MS), but also on the target tumor cells. Interestingly, the cytotoxic effect of these extracts on the normal human peripheral blood mononuclear cells (PBMC) was weak when compared to that induced on tumor cells. On the other hand, oral route treatment of P815 tumor-bearing mice (DBA2/P815) with propolis ethanolic extract (5 mg per mouse every fourth day, fi ve times for group A, and 2.5 mg per mouse every fourth day, fi ve times for group B) signifi cantly reduced the tumor volume (1.2 cm 3 for group A and 2.7 cm 3 for group B at the 22 nd day after tumor graft). These effects are statistically signifi cant as compared to those obtained with the control untreated mice (tumor volume 3.5 cm 3 at day 22).

Differential Cytotoxic Activity of Essential Oil of Lippia citriodora from Different Regions in Morocco

“…[27] [28] Briefly, cells were washed twice with PBS at a density of 1.5 9 10 5 cells/ml in flat-bottomed 96-well microtiter plates (Thermo Fisher Scientific) in 100 ll per well of complete medium. The used cell lines were obtained from the Biological Resource Center of Gustave Roussy Institute, Villejuif, France as a gift of Prof. G. Lemaire, Institute of Biochemistry, University of Paris XI, France.…”

Metabolite profiling, antioxidant and antibacterial activities of Brazilian propolis: Use of correlation and multivariate analyses to identify potential bioactive compounds

“…Many biological properties have been attributed to propolis, including anticancer (Mouse et al, 2012), antimicrobial (Popova, Silici, Kaftanoglu, & Bankova, 2005), antioxidant (Alves & Kubota, 2013), antileishmanial (Duran, Muz, Culha, Duran, & Ozer, 2011), wound healing (Batista et al, 2012;Guney, Karaman, Oner, & Yerer, 2011), anti-inflammatory and immunomodulatory activities (Araujo, Libério, Guerra, Ribeiro, & Nascimento, 2011). For example, Mouse et al (2012) investigated the chemical composition of Moroccan propolis extracts by HPLC/ESI-MS, as well as their in vitro and in vivo anticancer potential.…”

“…For example, Mouse et al (2012) investigated the chemical composition of Moroccan propolis extracts by HPLC/ESI-MS, as well as their in vitro and in vivo anticancer potential. It was shown that Moroccan propolis extracts contained several flavonoids, including rhamnetin, luteolin, and trihydroximethoxiflavone, some flavonoid glucosides, such as wogonoside, quercetin arabino-glucoside, and kaempferol O-glucoside and that the anticancer activity depended mainly on not only the chemical composition of the extracts, but also on the target tumor cells (Mouse et al, 2012). Using a GC-MS based approach, Duran et al (2011) investigated the relationship between the chemical composition extracts obtained from two Turkish propolis and their antileishmanial activity.…”

Insight on Propolis from Mediterranean Countries: Chemical Composition, Biological Activities and Application Fields

“…Other authors have found similar results as [255] for propolis from Algeria, which showed immunomodulatory and anti-inflammatory effect in animals with uveitis induced by α-tropomyosin. [293] Mouse et al [120] did a screening of the antitumor potential of Moroccan propolis extracts, from Beni Mellal region, in P815 tumor-bearing mice and have found that the treatment with propolis have the capacity to reduce the tumor volume as compared with the control. [120] Mountassir et al [294] have investigated samples from the same region regarding its possible antinociceptive activity and have conclude that propolis activated both central and peripheral mechanisms to elicit the analgesic effect in tested animals.…”