Evaluation of the In Vitro Wound-Healing Activity and Phytochemical Characterization of Propolis and Honey

Afonso, Alexandra M.; Gonçalves, Joana; Luís, Ângelo; Gallardo, Eugénia; Duarte, Ana Paula

doi:10.3390/app10051845

Cited by 23 publications

(22 citation statements)

References 27 publications

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“…An additive effect was instead observed in the scratch assay with a more significant wound closure compared to control. It has been hypothesised that these increased beneficial effects of honey might be explained by the improved antioxidant and anti-inflammatory activity upon propolis extract addition [118]. Another mechanism responsible for the ability to promote wound healing could be the propolis-mediated increased expression of AQP3 and consequent augmented Ca 2+ , a mechanism also observed in honey [87], as described earlier in this review.…”

Section: Latest Advances Of Honey Applications In Wound Carementioning

confidence: 57%

The Rediscovery of Honey for Skin Repair: Recent Advances in Mechanisms for Honey-Mediated Wound Healing and Scaffolded Application Techniques

2021

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Honey is a honey-bee product obtained mainly by the enzymatic processing of nectar from a variety of plants, which leads to the wide range of colours and flavours available on the market. These organoleptic and nutritional features are influenced by the chemical composition, which in turn depends on the botanical origin. Bioactive compounds account for honey beneficial activity in medical applications, which explains the extensive use of honey in ethno-pharmacology since antiquity, from cough remedies to dermatological treatments. Wound healing is one of the main therapeutic uses of honey, and various design options in pharmaceutical technology such as smart delivery systems and advanced dressings are currently being developed to potentiate honey’s valuable properties for better performance and improved final outcome. In this review, we will focus on the latest research that discloses crucial factors in determining what properties are most beneficial when considering honey as a medicinal product. We will present the most recent updates on the possible mechanisms responsible for the exceptional effects of this ageless therapeutical remedy on skin repair. Furthermore, the state-of-the-art in application techniques (incorporation into scaffolds as an alternative to direct administration) used to enhance honey-mediated wound-healing properties are explored.

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Section: Latest Advances Of Honey Applications In Wound Carementioning

confidence: 57%

The Rediscovery of Honey for Skin Repair: Recent Advances in Mechanisms for Honey-Mediated Wound Healing and Scaffolded Application Techniques

2021

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“…Afonso et al evaluated the in vitro wound healing activity by scratching assay on NHDF cell lines [ 37 ]. The rate of closing the wound was measured and cells migration demonstrated wound healing.…”

Section: Discussionmentioning

confidence: 99%

In Vitro and In Vivo Wound Healing Activity of Astragalus floccosus Boiss. (Fabaceae)

Akbari

Azadbakht

Bagheri

et al. 2022

Advances in Pharmacological and Pharmaceutical Sciences

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Estrogens are a group of sex hormones which have receptors on the skin and lead to increased cells and wound healing. Normally isoflavonoids are present in Astragalus floccosus Boiss. (Leguminosae). Therefore, the present study was conducted to evaluate the presence of isoflavonoids in A. floccosus’ rich fraction of flavonoid and evaluate its wound healing effect accordingly. Flavonoids were evaluated by LCMS. Scratch was conducted and the medium culture was treated with the Astragalus’ rich fraction of flavonoid (RFF) and was compared with nontreated culture during 48 hours. In addition, in vivo full-thickness wound healing evaluation was performed on rats. The rats were put into four groups and treated on a daily basis for 21 days with a cream containing 1.5% of the RFF (group 1), silver sulfadiazine (group 2), and Vaseline (group 3) separately. The nontreated group (group 4) was created for a better comparison. During the examination, wound size was evaluated and histopathological examination was performed. Herbal analysis detected 11 flavonoids, including 2 isoflavonoids, Calycosin-7-O-beta-D-glucoside and Formononetin, in the RFF. In vitro scratch wound healing showed significant improvement with RFF treatment in comparison to nontreated medium. Furthermore, in vitro drug release of Astragalus ointment showed a stationary line during 24 h and 0.14 mg/ml of flavonoid penetrated the skin. In vivo wound size evaluation showed significant improvement in the group treated with the RFF in comparison to other groups. Histopathological results indicated that congestion, edema, inflammation, necrosis, and angiogenesis decreased during the examination and fibroblast proliferation fibrosis epithelization was increased especially in the RFF group in comparison to the silver sulfadiazine and free groups. In conclusion, A. floccosus showed that wound healing activity in both in vitro and in vivo analyses can be attributed to the presence of isoflavonoids with estrogen-like activity in this plant.

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“…Furthermore, biogenic silver nanoparticles (Bio AgNP) with propolis showed antibacterial activity against Gram-and Gram + bacteria stimulating fibroblast cell proliferation and heal wound in surgical sutures [279]. In a study, propolis extract incorporated in a biocompatible polyurethane-hyaluronic acid (PU-HA) nanofiber in wound dressing, displayed a potent activity favouring the fibroblast cells adhesion, deposition of collagen fibers and cell proliferation in wounded areas [280,281]. A propolis mixture of two different geographical location evidenced better potential effect than individually on wound healing through re-epithelization process in rat chronic wound by bacterial inhibition [282].…”

Section: Wound Healing Activitymentioning

confidence: 99%

Propolis: An update on its chemistry and pharmacological applications

et al. 2022

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Propolis, a resinous substance produced by honeybees from various plant sources, has been used for thousands of years in traditional medicine for several purposes all over the world. The precise composition of propolis varies according to plant source, seasons harvesting, geography, type of bee flora, climate changes, and honeybee species at the site of collection. This apiary product has broad clinical applications such as antioxidant, anti-inflammatory, antimicrobial, anticancer, analgesic, antidepressant, and anxiolytic as well asimmunomodulatory effects. It is also well known from traditional uses in treating purulent disorders, improving the wound healing, and alleviating many of the related discomforts. Even if its use was already widespread since ancient times, after the First and Second World War, it has grown even more as well as the studies to identify its chemical and pharmacological features, allowing to discriminate the qualities of propolis in terms of the chemical profile and relative biological activity based on the geographic place of origin. Recently, several in vitro and in vivo studies have been carried out and new insights into the pharmaceutical prospects of this bee product in the management of different disorders, have been highlighted. Specifically, the available literature confirms the efficacy of propolis and its bioactive compounds in the reduction of cancer progression, inhibition of bacterial and viral infections as well as mitigation of parasitic-related symptoms, paving the way to the use of propolis as an alternative approach to improve the human health. However, a more conscious use of propolis in terms of standardized extracts as well as new clinical studies are needed to substantiate these health claims.

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Evaluation of the In Vitro Wound-Healing Activity and Phytochemical Characterization of Propolis and Honey

Cited by 23 publications

References 27 publications

The Rediscovery of Honey for Skin Repair: Recent Advances in Mechanisms for Honey-Mediated Wound Healing and Scaffolded Application Techniques

The Rediscovery of Honey for Skin Repair: Recent Advances in Mechanisms for Honey-Mediated Wound Healing and Scaffolded Application Techniques

In Vitro and In Vivo Wound Healing Activity of Astragalus floccosus Boiss. (Fabaceae)

Propolis: An update on its chemistry and pharmacological applications

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