Evaluation of the In Vitro and In Vivo Effects of Biglycan in Innate Immunity

Zeng-Brouwers, Jinyang; Huber, Lisa Sophie; Merline, Rosetta; Trebicka, Jonel; Wygrecka, Małgorzata; Schaefer, Liliana

doi:10.1007/978-1-0716-2946-8_9

Cited by 2 publications

(5 citation statements)

References 13 publications

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“…For instance, soluble BGN produced by the ECM fosters in ammatory responses through Toll-like receptors (TLRs) 2/TLR4 in macrophages and dendritic cells. 22 BGN activates the nuclear translocation of NF-κB in B cells, leading to the emergence of its transcriptional effects and the activation of p38 and ERK signaling cascades, thereby enhancing the expression of proin ammatory cytokines to trigger and regulate in ammation. 23 The expression of BGN is inversely correlated with CD8 + T cells in triple-negative breast cancer, suggesting that BGN may be involved in adaptive immune responses.…”

Section: Discussionmentioning

confidence: 99%

Study on the mechanism of BGN in progression and metastasis of ccRCC

Xia,

He,

et al. 2023

Preprint

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Purpose To investigate the role of BGN in the progression and metastasis of ccRCC. Methods Based on multiple public databases, we investigated the expression level of BGN in ccRCC, its clinical significance, and its association with immune cells. Real-time fluorescence quantitative PCR was employed to validate BGN expression in tumor and adjacent normal tissues. BGN knockdown cells were generated through lentiviral transfection to examine the impact of BGN on ccRCC. Cell proliferation, migration, and invasion were assessed using wound healing, transwell migration, and invasion assays, respectively. Differential gene analysis, GO-KEGG analysis, and GSEA analysis were performed by RNA sequencing to elucidate the underlying signaling pathways . Results Our findings from database analysis and polymerase chain reaction (PCR) revealed a significant upregulation of BGN expression in kidney cancer tissues compared to normal tissues. Further analysis demonstrated a correlation between high BGN expression and ccRCC progression and immune infiltration. In vitro experiments confirmed that BGN silencing effectively inhibited cell proliferation, migration, and invasion of ccRCC. Mechanistically, these effects may be mediated through the MAPK signaling pathway . Conclusion BGN potentially plays a pivotal role in the progression and metastasis of ccRCC, possibly acting through the MAPK signaling pathway. Therefore, BGN holds promise as a potential therapeutic target for ccRCC.

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Section: Discussionmentioning

confidence: 99%

Study on the mechanism of BGN in progression and metastasis of ccRCC

Xia,

He,

et al. 2023

Preprint

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Section: Biglycan Effects On Cancer-associated Inflammationmentioning

confidence: 99%

“…Some of the pro-tumorigenic effects of biglycan are reflected in its participation in inflammatory and cellular immune responses [147]. Notably, biglycan affects both innate and adaptive immunity mechanisms [147,148]. Thus, the ECM-derived soluble form of biglycan acts as a danger signal by triggering an inflammatory response via the toll-like receptor (TLR)2/TLR4 in macrophages and dendritic cells [147].…”

Section: Biglycan Effects On Cancer-associated Inflammationmentioning

confidence: 99%

“…Notably, biglycan affects both innate and adaptive immunity mechanisms [147,148]. Thus, the ECM-derived soluble form of biglycan acts as a danger signal by triggering an inflammatory response via the toll-like receptor (TLR)2/TLR4 in macrophages and dendritic cells [147]. According to molecular studies, the soluble protein acts as an ECM damage-associated molecular pattern (DAMP) that binds to innate immunity Toll-like receptors, i.e., TLR2 and TLR4, on the surface of macrophages [149].…”

Section: Biglycan Effects On Cancer-associated Inflammationmentioning

confidence: 99%

“…Biglycan also attaches to the CD14 co-receptor via its protein core. This interaction is an obligatory step for a biglycan-dependent inflammatory signaling cascade via TLR2 and TLR4 [147,150], which releases cytokines and chemokines and recruits inflammatory cells [151]. Thus, in a model of renal inflammation, hepatocytederived biglycan induces leucocyte recruitment and infiltration of the renal parenchyma by the TLR adapter protein MyD88 [70].…”

Section: Biglycan Effects On Cancer-associated Inflammationmentioning

confidence: 99%

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The Landscape of Small Leucine-Rich Proteoglycan Impact on Cancer Pathogenesis with a Focus on Biglycan and Lumican

Berdiaki

Giatagana

Tzanakakis

et al. 2023

Cancers

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Cancer development is a multifactorial procedure that involves changes in the cell microenvironment and specific modulations in cell functions. A tumor microenvironment contains tumor cells, non-malignant cells, blood vessels, cells of the immune system, stromal cells, and the extracellular matrix (ECM). The small leucine-rich proteoglycans (SLRPs) are a family of nineteen proteoglycans, which are ubiquitously expressed among mammalian tissues and especially abundant in the ECM. SLRPs are divided into five canonical classes (classes I–III, containing fourteen members) and non-canonical classes (classes IV–V, including five members) based on their amino-acid structural sequence, chromosomal organization, and functional properties. Variations in both the protein core structure and glycosylation status lead to SLRP-specific interactions with cell membrane receptors, cytokines, growth factors, and structural ECM molecules. SLRPs have been implicated in the regulation of cancer growth, motility, and invasion, as well as in cancer-associated inflammation and autophagy, highlighting their crucial role in the processes of carcinogenesis. Except for the class I SLRP decorin, to which an anti-tumorigenic role has been attributed, other SLPRs’ roles have not been fully clarified. This review will focus on the functions of the class I and II SLRP members biglycan and lumican, which are correlated to various aspects of cancer development.

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Evaluation of the In Vitro and In Vivo Effects of Biglycan in Innate Immunity

Cited by 2 publications

References 13 publications

Study on the mechanism of BGN in progression and metastasis of ccRCC

Study on the mechanism of BGN in progression and metastasis of ccRCC

The Landscape of Small Leucine-Rich Proteoglycan Impact on Cancer Pathogenesis with a Focus on Biglycan and Lumican

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