Evaluation of the in vivo anti malarial activity of the methanolic leaf extract of Nepata cateria

M, E Khan; J, O Amupitan; A, O Oyewale; Ndukwe, G

doi:10.5897/rpb2011.030

Cited by 3 publications

(1 citation statement)

References 25 publications

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“…Group VI received distilled water 10 ml kg-1 (Negative control). On day 5, the blood was collected from the tail of each mouse and smear made (Khan et al, 2015). The blood films was fixed with methanol, stained with 10% Giemsa at pH 7.2 for 10 min and parasitaemia determined microscopically (Peter et al, 1975).…”

Section: Antiplasmodial Activity Against Early Infection (Suppressive...mentioning

confidence: 99%

Modulation of Cytokine Expression and Antimalarial Activity of Residual Aqueous Fraction of the Methanol Leaf Extract of Ficus Asperifolia in Plasmodium Berghei Infected Mice

Abdullahi,

Gudaji,

Yaro

2024

FJS

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Malaria is an inflammatory cytokine driven disease that sometimes results in death due to lyses of red blood cells. The disease presents a major health challenge to the world especially in tropical and subtropical areas. The aim of this research was to evaluate the modulation of cytokine expression and in vivo antimalarial activity of the residual aqueous fraction (RAF) of methanol leaf extract of Ficus asperifolia (FaMLE). The extract was dissolved in distilled water and serially partitioned with solvents of different polarity to obtain the residual aqueous fraction. Standard protocol was used to determine the preliminary phytochemical and OECD method was used to evaluate the acute toxicity of the extracts. The antimalarial activity was evaluated in mice infected with chloroquine sensitive plasmodium berghei. Oral LD50 of the extracts were estimated to be >5000 mg/kg. The RAF at all doses produced a significant (p<0.05) suppressive, prophylactic, and curative antimalarial activity. The extract also significantly prolonged the survival time of the treated mice compared to the distilled water group. There was a statistically significant (p<0.05) reduction in the mean values of pro inflammatory cytokines assayed (IL-1β, TNFα and IFN-γ) in the RAF treated groups compared with the distilled water treated group. In conclusion RAF possesses antimalarial activity which might have been achieved through modulation of cytokine expression among other possible mechanisms.

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Section: Antiplasmodial Activity Against Early Infection (Suppressive...mentioning

confidence: 99%

Modulation of Cytokine Expression and Antimalarial Activity of Residual Aqueous Fraction of the Methanol Leaf Extract of Ficus Asperifolia in Plasmodium Berghei Infected Mice

Abdullahi,

Gudaji,

Yaro

2024

FJS

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Oral acute toxicity and antimalarial potentials of aqueous and methanolic extracts of roots, leaves and stem of Dictyandra arborescens (Welw.) on Plasmodium berghei infected mice

et al. 2021

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Background Malaria is one of the tropical diseases of universal concern particularly with continuous appearance of resistant strains of P.falciparum. This calls for continous screening of traditional plants such that new and effective antimalarial agents will be developed. This study therefore explored the oral acute toxicity and antimalarial potentials of aqueous and methanolic extracts of roots, leaves and stem of Dictyandra arborescens on Plasmodium berghei infected mice. Results No mortality was recorded in any of the experimental animal groups even at the highest tested dose (5000 mg/kg b.wt) of the extract after monitoring them for 4hrs and subsequently for 7 days. Out of the six extracts, methanolic extracts of the roots and leaves exhibited more antimalarial activity than others. A significant difference (P < 0.05) was statistically observed in the parasite count of groups that received methanol extracts of roots and leaves of D. arborescens. This observation was made when these two extracts were compared with other groups as well as the negative control. However, activity of the standard antimalarial drug (artesunate) was higher (p˂0.05) than those of the extracts. Phytochemicals such as tannins, alkaloids, saponins, terpenoids, flavonoids etc. were present in the extracts in varying quantities. GC–MS analysis of methanol extract of the root of this plant showed different chemical compounds. Conclusion Administration of aqueous and methanol extracts of roots, leaves and stem of D. arborescens in mice is not harmful at any dose less than or equal to 5000 mg/kg. Methanol extracts exhibited more antimalarial activity than aqueous extracts suggesting that antimalarial activity of the plant parts could be affected by the solvent used for extraction and antimalarial activity may be more in a particular part of a plant. The presence of different bioactive compounds identified in phytochemical and GC–MS analysis could be the fundamental scientific evidence for the antimalarial activity exhibited by this plant especially in the root.

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Biochemical and haematological enhancement activities of aqueous and methanol leaves, stem and roots extracts of Chasmanthera dependens (Hochst) and Dictyandra arborescens (Welw.)

et al. 2021

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Background Africa is blessed with rich floral biodiversity which are harnessed as herbal medicines and remedies for several ailments. Since there has been evidence of organ toxicity following prolonged ingestion of medicinal plant concotions, this study investigated the biochemical and hematological enhancement activities of aqueous and methanolic leaves, stem and roots extracts of Chasmanthera dependens (Hochst) and Dictyandra arborescens (Welw) in adult male albino rats. Results Leaves, stem and roots extracts of D. arborescens, as well as extracts of leaves and roots of C. dependens did not record any mice mortality even at 5000 mg/kg b.wt but extracts of C. dependens stem in both medium recorded death at 2900 mg/kg b. wt and 5000 mg/kg b.wt. Ingestion of the extracts by rats over a 14-day period increased (p < 0.05) the body weights of the experimental animals in the C. dependens and D. arborescens treated groups. The relative organ weights of rats that received the extracts did not differ (p < 0.05) significantly from those of the standard and normal control. Administration of the extracts revealed significant (p < 0.05) increase in haematological parameters (PCV, Hb, RBCs, WBCs, MCH, Neutrophils, Lymphocytes and MCHC) at 200 mg/kg−1 body weight. For biochemical parameters, levels of ALT, AST, ALP, total protein, and albumin were not significantly (p < 0.05) elevated following administration of the extracts. Conclusion These parameters did not differ significantly from the normal and standard control. Since these extracts did not exhibit any chronic toxicity on experimental animals, suggesting no harmful effects following their use, their continuous use in ethno-medicine is therefore justified.

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Evaluation of the in vivo anti malarial activity of the methanolic leaf extract of Nepata cateria

Cited by 3 publications

References 25 publications

Modulation of Cytokine Expression and Antimalarial Activity of Residual Aqueous Fraction of the Methanol Leaf Extract of Ficus Asperifolia in Plasmodium Berghei Infected Mice

Modulation of Cytokine Expression and Antimalarial Activity of Residual Aqueous Fraction of the Methanol Leaf Extract of Ficus Asperifolia in Plasmodium Berghei Infected Mice

Oral acute toxicity and antimalarial potentials of aqueous and methanolic extracts of roots, leaves and stem of Dictyandra arborescens (Welw.) on Plasmodium berghei infected mice

Biochemical and haematological enhancement activities of aqueous and methanol leaves, stem and roots extracts of Chasmanthera dependens (Hochst) and Dictyandra arborescens (Welw.)

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