Evaluation of the influence of arsenic species on the nitrogen metabolism of a model angiosperm: nasturtium,Tropaeolum majus

Schmidt, Anne-Christine; Mattusch, Jürgen; Reißer, Werner; Wennrich, Rainer

doi:10.1002/aoc.869

Cited by 8 publications

(4 citation statements)

References 46 publications

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“…response of N metabolism to As treatment differs in a variety-and time-dependent manner [30,75]. On the other hand, in another article [76] the effects of As on N metabolism were consistent with our study. We found that increased N uptake by ferns was affected by As, as confirmed by the correlations (S1 Table).…”

Section: Plos Onesupporting

confidence: 92%

“…We found that increased N uptake by ferns was affected by As, as confirmed by the correlations (S1 Table). Arsenite-treated plants exhibited elevated concentrations of non-protein 15 N, which could indicate either the stimulated uptake of nitrate or an interruption of amino acid/protein synthesis [76]. In this article, the activation/deactivation of N metabolism is regarded as a symptom of the alarm phase of stress response in plants, when general metabolic stimulation/ inhibition occurs [76].…”

Section: Plos Onementioning

confidence: 99%

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Response of cytokinins and nitrogen metabolism in the fronds of Pteris sp. under arsenic stress

et al. 2020

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Given the close relationship between cytokinins (CKs), photosynthesis and nitrogen metabolism, this study assessed the effect of arsenic (As) contamination on these metabolic components in the As-hyperaccumulators Pteris cretica L. var. Albo-lineata (Pc-A) and var. Parkerii (Pc-P) as well as the As-non-hyperaccumulator Pteris straminea Mett. ex Baker (Ps). The ferns were cultivated in a pot experiment for 23 weeks in soil spiked with As at the levels 20 and 100 mg�kg-1. For the purpose of this study, the CKs were placed into five functionally different groups according to their structure and physiological roles: bioactive forms (bCKs; CK free bases); inactive or weakly active forms (dCKs; CK N-glucosides); transport forms (tCKs; CK ribosides); storage forms (sCKs; O-glucosides); and primary products of CK biosynthesis (ppbCKs; CK nucleotides). An important finding was higher CKs total content, accumulation of sCKs and reduction of dCKs in As-hyperaccumulators in contrast to non-hyperaccumulator ferns. A significant depletion of C resources was confirmed in ferns, especially Ps, which was determined by measuring the photosynthetic rate and chlorophyll fluorescence. A fluorescence decrease signified a reduction in the C/N ratio, inducing an increase of bioactive CKs forms in Pc-P and Ps. The impact of As on N utilization was significant in As-hyperaccumulators. The glutamic acid/glutamine ratio, an indicator of primary N assimilation, diminished in all ferns with increased As level in the soil. In conclusion, the results indicate a large phenotypic diversity of Pteris species to As and suggest that the CKs composition and the glutamic acid/glutamine ratio can be used as a tool to diagnose As stress in plants.

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Section: Plos Onesupporting

confidence: 92%

Section: Plos Onementioning

confidence: 99%

Response of cytokinins and nitrogen metabolism in the fronds of Pteris sp. under arsenic stress

et al. 2020

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“…In living organisms such a cleavage of the bond between As and C was found for plants treated with phenylarsonic acids. 7 It is assumed that m/z 476 ( Fig. 2(a)) represents an additive complex between glutathione and PAO.…”

Section: Resultsmentioning

confidence: 99%

Mass spectrometric evidence for different complexes of peptides and proteins with arsenic(III), arsenic(V), copper(II), and zinc(II) species

Schmidt

Koppelt

Neustadt

et al. 2006

Rapid Comm Mass Spectrometry

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Trivalent and pentavalent arsenic were incubated with sulfur-containing amino acid, peptide and protein solutions both as organic compounds (phenylarsine oxide, phenylarsonic acid, dimethylarsinic acid, monomethylarsonic acid) and as inorganic compounds (arsenite, As(III), and arsenate, As(V)). After incubation of phenylarsine oxide solutions with cysteine and glutathione the mass spectra showed a covalent bond between arsenic and sulfur, which was stable at both acidic and neutral pH values. The mass spectra were dominated by monovalent ions at m/z 272 for cysteine samples and at m/z 458 for glutathione samples. Based on these masses the ionic structures could be ascribed to either fragment ions of the covalent arsenic-sulfur complexes or to other arsenic-bonding sites presumably at the amino group. Interestingly, under the same conditions no interactions of inorganic arsenite or arsenate could be measured. In the presence of added Cu(2+) ions all mass signals caused by a reaction of phenylarsine oxide with glutathione disappeared. In these mass spectra only the oxidised form of glutathione (GSSG) was found because of the redox activity of Cu(II). For the model protein lysozyme, no interactions with arsenic could be detected, whereas definite Cu- and Zn-lysozyme complexes with a stoichiometry of 1:1 and 2:1 for Zn(2+) ions and Cu(2+) ions, respectively, were observed. In contrast, for thioredoxin a bonding of As that depended on the concentration of the disulfide-reducing agent tris(2-carboxyethyl) phosphine was demonstrated. For three different phenylarsonic acids and for dimethylarsinic acid that all contain pentavalent arsenic, complexes with glutathione appeared in the mass spectra, which can be attributed to non-covalent interactions or to a covalent bond caused by an additive reaction. The optimisation of the experimental conditions necessary for the mass spectrometric analysis of the interactions of the arsenic species with peptides and proteins is described and the obtained mass spectra that provide information on the kinds of bonds are discussed.

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“…Nevertheless, there are some reports of isotope discrimination in metal-exposed plants: dimethylarsinate decreased δ 15 N in nasturtium (Tropaelum majus) while salinity increased δ 13 C in beans. 23,24 The stable isotope ratio reflects the amount in which heavier isotopes are discriminated with respect to lighter isotopes, and changes in isotope composition is a sensitive measure of the physiological and biochemical responses of plants to different environmental conditions. 25,26 For example, C and N isotope discrimination in metal-exposed plants (i.e.…”

Section: And N Stable Isotope Analysismentioning

confidence: 99%

Intergenerational responses of wheat (Triticum aestivum L.) to cerium oxide nanoparticles exposure

Rico

Johnson

Marcus

et al. 2017

Environ. Sci.: Nano

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The intergenerational impact of engineered nanomaterials in plants is a key knowledge gap in the literature. A soil microcosm study was performed to assess the effects of multi-generational exposure of wheat (Triticum aestivum L.) to cerium oxide nanoparticles (CeO 2 -NPs). Seeds from plants that were exposed to 0, 125, and 500 mg CeO 2 -NPs kg −1 soil (Ce-0, Ce-125 or Ce-500, respectively) in first generation (S1) were cultivated in factorial combinations of Ce-0, Ce-125 or Ce-500 to produce second generation (S2) plants.The factorial combinations for first/second generation treatments in Ce-125 were S1-Ce-0/S2-Ce-0, S1-Ce-0/S2-Ce-125, S1-Ce-125/S2-Ce-0 and S1-Ce-125/S2-Ce-125, and in Ce-500 were S1-Ce-0/S2-Ce-0, S1-Ce-0/S2-Ce-500, S1-Ce-500/S2-Ce-0 and S1-Ce-500/S2-Ce-500. Agronomic, elemental, isotopic, and synchrotron X-ray fluorescence (XRF) and X-ray absorption near-edge spectroscopy (XANES) data were collected on second generation plants. Results showed that plants treated during the first generation only with either Ce-125 or Ce-500 (e.g. S1-Ce-125/S2-Ce-0 or S1-Ce-500/S2-Ce-0) had reduced accumulation of Ce (61 or 50%), Fe (49 or 58%) and Mn (34 or 41%) in roots, and δ 15 N (11 or 8%) in grains compared to the plants not treated in both generations (i.e. S1-Ce-0/S2-Ce-0). Plants treated in both generations with Ce-125 (i.e. S1-Ce-125/S2-Ce-125) produced grains that had lower Mn, Ca, K, Mg and P relative to plants treated in the second generation only (i.e. S1-Ce-0/S2-Ce-125). In addition, synchrotron XRF elemental chemistry maps of soil/plant thin-sections revealed limited transformation of CeO 2 -NPs with no evidence of plant uptake or accumulation. The findings demonstrated that first generation exposure of wheat to CeO 2 -NPs affects the physiology and nutrient profile of the second generation plants. However, the lack of concentration-dependent responses indicate that complex physiological processes are involved which alter uptake and metabolism of CeO 2 -NPs in wheat.

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Evaluation of the influence of arsenic species on the nitrogen metabolism of a model angiosperm: nasturtium,Tropaeolum majus

Cited by 8 publications

References 46 publications

Response of cytokinins and nitrogen metabolism in the fronds of Pteris sp. under arsenic stress

Response of cytokinins and nitrogen metabolism in the fronds of Pteris sp. under arsenic stress

Mass spectrometric evidence for different complexes of peptides and proteins with arsenic(III), arsenic(V), copper(II), and zinc(II) species

Intergenerational responses of wheat (Triticum aestivum L.) to cerium oxide nanoparticles exposure

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