Evaluation of the interferon-γ release assay in healthcare workers

Nienhaus, Albert; Schablon, Anja; Bâcle, Colette Le; Siano, B.; Diel, Roland

doi:10.1007/s00420-007-0212-1

Cited by 63 publications

(58 citation statements)

References 20 publications

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“…We identified a prior TST as an independent factor associated with false reactivity of TST consistent with reports from previous studies (27,28). However, we did not find a significant correlation between the TST reaction size and the duration since the last performed TST.…”

Section: Discussionsupporting

confidence: 91%

Tuberculin Skin Test and QuantiFERON<sup>®</sup>-TB Gold In-Tube Test for Diagnosing Latent Tuberculosis Infection among Thai Healthcare Workers

Khawcharoenporn

Apisarnthanarak

Sangkitporn³

et al. 2016

Jpn J Infect Dis

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SUMMARY:A cross-sectional study was conducted on the performance of the tuberculin skin test (TST) and QuantiFERON -TB Gold In-Tube test (QFT-IT) for detecting latent tuberculosis infection among Thai healthcare workers (HCWs). Each HCW underwent both the TST and QFT-IT during the annual health screening. Among the 260 HCWs enrolled, the median age was 30 years (range 19-60 years), 92z were women, 64z were nurses and nurse assistants, 78z were Bacillus Calmette Gu áerin vaccinated, and 37z had previously taken the TST. Correlation between TST reaction size and the interferon-g level was weak (r ＝ 0.29; P ＜ 0.001). Of the HCWs, 38z and 20z had a reactive TST and a positive QFT-IT, respectively. Using QFT-IT positivity as a standard for latent tuberculosis diagnosis, the cut-off for TST reactivity with the best performance was 13 mm with a sensitivity, specificity, false positivity, and false negativity of 71z, 70z, 30z, and 29z, respectively (area under the curve 0.73; P ＜ 0.001). The independent factor associated with a false reactive TST was a previous TST (adjusted odds ratio 1.83; P ＝ 0.04). Our findings suggest that the QFT-IT may be the preferred test among HCWs with previous TST. In settings where the QFT-IT is not available, appropriate cut-offs for TST reactivity should be evaluated for use among HCWs.

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Section: Discussionsupporting

confidence: 91%

Tuberculin Skin Test and QuantiFERON<sup>®</sup>-TB Gold In-Tube Test for Diagnosing Latent Tuberculosis Infection among Thai Healthcare Workers

Khawcharoenporn

Apisarnthanarak

Sangkitporn³

et al. 2016

Jpn J Infect Dis

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“…Positive IFN-γ tests were associated with age and degree of occupational exposure, but not with BCG vaccination, a finding consistent with previous studies with QFT-G tests. 12,13 Although IFN-γ-based tests have became a good alternative to TST for serial testing of HCWs, factors such as reversions and conversions should be taken into account. Choi et al 14 described conversions of QFT-IT in HCWs 2-4 weeks after performing a TST test among TST reactors, but not among non-reactive individuals.…”

Section: Health Care Workersmentioning

confidence: 99%

Diagnosis of tuberculosis infection using interferon-γ-based assays

Cerezales

Benítez

2011

Enfermedades Infecciosas y Microbiología Clínica

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“…So far, only a few systematic investigations of LTBI in HCWs using the IGRA have been published [8][9][10][11][12][13][14]. Therefore, we studied the prevalence of LTBI and active TB in Portuguese HCWs and compared IGRA and TST performance.…”

mentioning

confidence: 99%

Tuberculosis screening in Portuguese healthcare workers using the tuberculin skin test and the interferon-γ release assay

Costa

Sá²,

Cardoso³

et al. 2009

Eur Respir J

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The prevalence of latent tuberculosis (TB) infection (LTBI) and the incidence of active tuberculosis in healthcare workers (HCWs) in a Portuguese hospital were examined.This cross-sectional study comprises 4,735 hospital workers screened between May 2005 and September 2008. Tuberculin skin test (TST) and interferon-c release assay (IGRA) were used simultaneously in 1,219 HCWs (25.7%). Radiographs were taken in symptomatic HCWs or in testpositive HCWs. The tests were repeated annually or bi-annually depending on risk assessment.IGRA was positive in 32.6% and TST in 74.2% of the HCWs. Years spent in healthcare were a risk factor for a positive IGRA, but not for a positive TST. Repeated bacillus Calmette-Guérin vaccination increased the probability of TST+/IGRA-discordance (35.4% versus 54.4%, respectively). In those tested three times with TST during the study period (n559), the mean diameter of TST increased from 5 to 7 to 10 mm. Within 3 yrs, 31 HCWs were diagnosed with active TB (annual incidence rate 191 out of 100,000 people). In eight HCWs with active TB, TST and IGRA were performed at the time of diagnosis and each test was positive.TB burden in HCWs in Portugal is high. With IGRA, the number of radiographs needed to exclude active TB could have been reduced by about half without missing a case of active TB. Therefore IGRA should be introduced into TB screening programmes.

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Evaluation of the interferon-γ release assay in healthcare workers

Abstract: The data indicate that the IGRA should replace the TST in serial testing of HCW in high income, low incidence countries. The recommendations to use the IGRA in order to verify a positive TST only should be reconsidered.

Cited by 63 publications

References 20 publications

Tuberculin Skin Test and QuantiFERON<sup>®</sup>-TB Gold In-Tube Test for Diagnosing Latent Tuberculosis Infection among Thai Healthcare Workers

Tuberculin Skin Test and QuantiFERON<sup>®</sup>-TB Gold In-Tube Test for Diagnosing Latent Tuberculosis Infection among Thai Healthcare Workers

Diagnosis of tuberculosis infection using interferon-γ-based assays

Tuberculosis screening in Portuguese healthcare workers using the tuberculin skin test and the interferon-γ release assay

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Evaluation of the interferon-γ release assay in healthcare workers

Abstract: The data indicate that the IGRA should replace the TST in serial testing of HCW in high income, low incidence countries. The recommendations to use the IGRA in order to verify a positive TST only should be reconsidered.

Cited by 63 publications

References 20 publications

Tuberculin Skin Test and QuantiFERON<sup>&reg;</sup>-TB Gold In-Tube Test for Diagnosing Latent Tuberculosis Infection among Thai Healthcare Workers

Tuberculin Skin Test and QuantiFERON<sup>&reg;</sup>-TB Gold In-Tube Test for Diagnosing Latent Tuberculosis Infection among Thai Healthcare Workers

Diagnosis of tuberculosis infection using interferon-γ-based assays

Tuberculosis screening in Portuguese healthcare workers using the tuberculin skin test and the interferon-γ release assay

Contact Info

Product

Resources

About

Tuberculin Skin Test and QuantiFERON<sup>®</sup>-TB Gold In-Tube Test for Diagnosing Latent Tuberculosis Infection among Thai Healthcare Workers

Tuberculin Skin Test and QuantiFERON<sup>®</sup>-TB Gold In-Tube Test for Diagnosing Latent Tuberculosis Infection among Thai Healthcare Workers