Evaluation of the IS6110 PCR assay for the rapid diagnosis of tuberculous meningitis

Deshpande, Poonam S.; Kashyap, Rajpal S.; Ramteke, Suman; Nagdev, Khushboo J.; Purohit, Hemant J.; Taori, Girdhar M.; Daginawala, Hatim F.

doi:10.1186/1743-8454-4-10

Cited by 50 publications

(43 citation statements)

References 35 publications

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“…The study was relatively large (31 definite TBM cases) compared to those in published reports, where the numbers of cultureconfirmed CSF samples were relatively small (17-21). The sensitivity was not improved and remained suboptimal despite our use of fairly large volumes of CSF (500 l for the Amplicor PCR and 1,000 l for the Xpert MTB/RIF) compared to those in previous studies (22)(23)(24)(25)(26). A meta-analysis by Solomons et al confirmed sensitivities varying from 33% to 67% (27) Previously cited reasons for the suboptimal sensitivity have included the presence of inhibitors in the CSF, paucibacillary samples below the detection limit of the assay, and the aliquot phenomenon (i.e., the initial CSF sample taken may have fewer bacilli than those taken later).…”

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confidence: 76%

Comparison of Amplicor and GeneXpert MTB/RIF Tests for Diagnosis of Tuberculous Meningitis

et al. 2014

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confidence: 76%

Comparison of Amplicor and GeneXpert MTB/RIF Tests for Diagnosis of Tuberculous Meningitis

et al. 2014

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“…Also, we evaluated these two Hsps simultaneously to study the combined positivity and negativity. In addition to that, we have compared the results of M. tb Hsp 71 with that of the in house polymerase chain reaction (PCR) assay (Deshpande et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Mycobacterium tuberculosis 16 and 71 Heat Shock Proteins in the Cerebrospinal Fluid of Tuberculous Meningitis Patients

Shekhawat

2012

BJMMR

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Aims: To evaluate the efficiency of two potential Mycobacterium tuberculosis (M. tb) heat shock proteins (Hsps) towards the improvement of tuberculous meningitis (TBM) diagnosis. Study Design: The patients were divided into TBM (confirmed and suspected) and non TBM group. The cerebrospinal fluid (CSF) was collected and evaluated for M. Research ArticleBritish Journal of Medicine & Medical Research, 2(2): 243-253, 2012 244 with PCR technique and concordance was also calculated. Of the Hsp ELISA positive group for M. tb Hsp71, 24 were PCR positive and 2 were PCR negative with the 92.30 % concordance in TBM patients and in non TBM patients the concordance was observed to be 93.30%. Use of the monoclonal antibody Hsp 71 appear preferable over individual use of M. tb Hsp 16 and combined use of both Hsp and yield optimum results. Conclusion: Our data suggest that the detection of M. tb Hsp 71 in the CSF sample of TBM patients can be useful for the diagnosis of TBM patients. These predictors, however, need further work to validate reliability.

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“…2) Recently, another study that performed PCR using primers directed against the IS6110 gene reported the sensitivity of 91.4% and specificity of 75.9% for the diagnosis of tuberculous meningitis. 5) Both QFT and PCR have advantages and limitations. Given their advantages and non-invasive nature, we consider that QFT and PCR should be performed whenever intracranial tuberculoma is suspected.…”

Section: Discussionmentioning

confidence: 99%

Intracranial Tuberculoma in Non-immunosuppressive State

Matsumoto

Aikawa

Narita

et al. 2013

Neurol. Med. Chir.(Tokyo)

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The rise in the incidence of tuberculosis is generally related to human immunodeficiency virus infection. However, intracranial tuberculoma, a complication of tuberculosis considered to be a critical disease, can develop even in the absence of immunosuppressive state. Here, we describe 2 cases of intracranial tuberculoma occurring in patients with no evidence of immunosuppressive state or past history of tuberculosis. In Case 1, lesions were observed in the right lateral ventricle, with histological examination revealing granulomatous lesions. In Case 2, scattered lesions were observed in the cranium and the lung fields. In both cases, the QuantiFERON Test (QFT) was positive, and improvements were observed in the symptoms following administration of antituberculous drugs. Intracranial tuberculoma cannot be considered rare, and needs to be included in the differential diagnosis of intracranial lesions. Diagnosis can be tricky since this disease can develop in a patient in a non-immunosuppressive state or without a past history of tuberculosis. The QFT is an effective test to enable the diagnosis of tuberculomas in atypical patients.

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Evaluation of the IS6110 PCR assay for the rapid diagnosis of tuberculous meningitis

Cited by 50 publications

References 35 publications

Comparison of Amplicor and GeneXpert MTB/RIF Tests for Diagnosis of Tuberculous Meningitis

Comparison of Amplicor and GeneXpert MTB/RIF Tests for Diagnosis of Tuberculous Meningitis

Evaluation of Mycobacterium tuberculosis 16 and 71 Heat Shock Proteins in the Cerebrospinal Fluid of Tuberculous Meningitis Patients

Intracranial Tuberculoma in Non-immunosuppressive State

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