Evaluation of the Lactobacillus gasseri K7 and LF221 strains in weaned piglets for their possible probiotic use and their detection in the faeces

Abstract

-Lactobacillus gasseri LF221 and K7 are human isolates that were recognised in previous studies as potential probiotics. In the present study, the detection of LF221 or K7 strains in the faeces as well as their effects on the faecal coliform and lactobacilli counts and on the production parameters were studied in 18 weaned piglets. The animals were divided into three groups: an untreated control group and two groups dosed for 14 days, Lb. gasseri K7 or LF221. The experimental period lasted 25 days. For the discrimination among strains LF221, K7 and other faecal microflora, a combined approach that included culturing on selective media, testing of antimicrobial activity and random amplified polymorphic DNA (RAPD) analysis was used. The administration of a single probiotic strain (a daily dose of 5 × 10 10 cfu per piglet) did not significantly influence the viable counts of the coliforms (P > 0.05). A significantly higher number of lactobacilli in comparison with the control group was found in the K7 group after 15 days of probiotic bacteria administration (P = 0.02) but not in the LF221 group. The probiotic treatment did not have a significant influence on feed intake (P > 0.05) and weight gain (P > 0.05). The feed conversion efficiency in the K7 treated group during the whole period was significantly more favourable (P < 0.05) than in the non-treated control group (1.51 and 1.87, respectively). All the piglets remained healthy and no case of diarrhoea was observed. The LF221 and K7 strains survived the passage through the intestines and were successfully detected in the faeces. The colonies identical to the LF221 and K7 strains were isolated only from the faeces of animals fed with the LF221 or K7 strain, respectively. weaned pigs / probiotics / Lactobacillus gasseri / antimicrobial activity / RAPD Résumé -Utilisation des souches de lactobacillus gasseri K7 et LF221 comme probiotiques chez les porcelets sevrés et leur détection dans les fèces. Lactobacillus gasseri LF221 et K7 sont des isolats d'origine humaine qui ont été identifiés, dans des études précédentes, en tant que probiotiques potentiels. Dans cette étude, la détection des souches LF221 ou K7 dans les fèces, leurs effets sur le nombre de coliformes et lactobacilles fécaux, et sur les paramètres de production ont

Effects of Feeding of Two Potentially Probiotic Preparations from Lactic Acid Bacteria on the Performance and Faecal Microflora of Broiler Chickens

“…Thus, in the second experiment, slighter and subclinic necrotic enteritis could occur, as this type of enteritidis is asymptomatic [40]. Consequently, it is probable that the nutritional stress induced in this trial was not enough to see the growth-stimulating effects of the probiotic bacteria in animal exposed to stress reported by other authors [41, 42]. …”

Survival and in vivo adhesion of human isolates Lactobacillus gasseri LF221 and K7 in weaned piglets and their effects on coliforms, clostridia and lactobacilli viable counts in faeces and mucosa

“…The identity of the colonies grown on MRS agar with rifampicin was confirmed by RAPD as described previously (Bogovič Matijašić et al 2004). Some small colonies grown on rifampicin supplemented MRS agar plates and easily distinguishable from LF221 and K7, were also confirmed to be different by RAPD analysis.…”

“…RAPD analysis of selected colonies from MRS+rifampicin agar was performed as described previously (Bogovič Matijašić et al 2004) by PCR, applying primers designed by Tynkkynen et al (1999).…”

“…gasseri LF221 and K7 were found to survive the passage through piglets' intestines (Bogovič Matijašić et al 2004). gasseri LF221 and K7 were found to survive the passage through piglets' intestines (Bogovič Matijašić et al 2004).…”

Lactobacillus gasseri LF221 and K7 — from isolation to application

“…the average feed conversion efficiency calculated for the whole experimental period was significantly improved in the group given the K7 strain (Bogovič Matijašić et al, 2004).…”