Evaluation of the Leishmania recombinant K39 antigen as a diagnostic marker for canine leishmaniasis and validation of a standardized enzyme-linked immunosorbent assay

Scalone, Aldo; Luna, R. De; Oliva, Gætano; Baldi, Loredana; Satta, G.; Vesco, G.; Mignone, Walter; Turilli, C.; Mondesire, Roy; Simpson, D. M.; Donoghue, A.R; Frank, G.R; Gradoni, Luigi

doi:10.1016/s0304-4017(01)00643-4

Cited by 91 publications

(87 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Culling of seroreactive dogs from AVL-endemic areas for the control of the disease is a polemic subject because of the disagreement among investigators regarding the accuracy of the tests currently employed for this purpose 1,7,27,28 . There is no scientific evidence demonstrating the impact of dog culling on human infection.…”

Section: Discussionmentioning

confidence: 99%

Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples

Figueiredo

Madeira

Nascimento

et al. 2010

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

SUMMARYThe Brazilian Ministry of Health recommends the culling and euthanasia of dogs with a positive serological test for canine visceral leishmaniasis (CVL). In the Municipality of Rio de Janeiro, the technique used for the diagnosis of CVL is the indirect fluorescent antibody test (IFAT), using blood samples eluted on filter paper (eluate). A dog survey was conducted over a period of one year in the region of Carapiá, in order to evaluate the diagnosis of CVL in this region. All animals underwent clinical examination, and blood samples (serum and eluate) were collected for analysis by enzyme immunoassay (ELISA) and IFAT. A skin biopsy was obtained for parasitological examination (culture). A total of 305 animals were studied and Leishmania chagasi was isolated from nine animals. Sensitivity and specificity were 100% and 96.6% for ELISA, respectively, 100% and 65.5% for IFAT (cut-off at a 1:40 dilution), 100% and 83.4% for IFAT (cut-off at a 1:80 dilution), and 22.2% and 97.0% for eluate IFAT. In conclusion, ELISA was the best tool for the diagnosis of CVL among the serological techniques tested. The present results suggest the need for a better evaluation of filter paper IFAT as the only diagnostic method for CVL in the Municipality of Rio de Janeiro.

show abstract

Section: Discussionmentioning

confidence: 99%

Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples

Figueiredo

Madeira

Nascimento

et al. 2010

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

show abstract

“…[13][14][15][16] However, because most persons with the disease produces antibodies against Leishmania , diagnoses of clinically suspected human cases are often confirmed or the infection in dogs is indicated by serologic immunoassays. [17][18][19][20][21][22][23] These assays are carried out mainly with antigens from cultured Leishmania promastigote forms, which may also react with antibodies associated with other infectious diseases such as Chagas' disease and malaria, and thus produce falsepositive results. 24 In the past two decades, there has been a considerable effort to produce defined antigens, especially recombinant antigens, to be used in the serodiagnosis of human and canine VL.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Novel Leishmania infantum Recombinant Proteins Encoded by Genes from Five Families with Distinct Capacities for Serodiagnosis of Canine and Human Visceral Leishmaniasis

Oliveira¹,

Magalhães²,

Teixeira³

et al. 2011

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

To expand the available panel of recombinant proteins that can be useful for identifying Leishmania-infected dogs and for diagnosing human visceral leishmaniasis (VL), we selected recombinant antigens from L. infantum, cDNA, and genomic libraries by using pools of serum samples from infected dogs and humans. The selected DNA fragments encoded homologs of a cytoplasmic heat-shock protein 70, a kinesin, a polyubiquitin, and two novel hypothetical proteins. Histidine-tagged recombinant proteins were produced after subcloning these DNA fragments and evaluated by using an enzyme-linked immunosorbent assays with panels of canine and human serum samples. The enzyme-linked immunosorbent assays with different recombinant proteins had different sensitivities (67.4–93.0% and 36.4–97.2%) and specificities (76.1–100% and 90.4–97.3%) when tested with serum samples from Leishmania-infected dogs and human patients with VL. Overall, no single recombinant antigen was sufficient to serodiagnosis all canine or human VL cases.

show abstract

“…The ICT assay is based on recombinant antigens and has been developed into a dipstick format. The tests are based on recombinant antigens for which previous reports have demonstrated superior performance over traditional serological screening tests based on crude antigens or whole organisms (Scalone et al, 2002). Canine sera were tested for antibodies to recombinant K39 (rK39), which is an amastigote protein specific to visceralizing Leishmania spp.…”

mentioning

confidence: 99%

Surveillance for Antibodies to Leishmania spp. in Dogs from Sri Lanka

Rosypal¹,

Tripp²,

Kinlaw³

et al. 2010

Journal of Parasitology

View full text Add to dashboard Cite

Evaluation of the Leishmania recombinant K39 antigen as a diagnostic marker for canine leishmaniasis and validation of a standardized enzyme-linked immunosorbent assay

Cited by 91 publications

References 25 publications

Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples

Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples

Characterization of Novel Leishmania infantum Recombinant Proteins Encoded by Genes from Five Families with Distinct Capacities for Serodiagnosis of Canine and Human Visceral Leishmaniasis

Surveillance for Antibodies to Leishmania spp. in Dogs from Sri Lanka

Contact Info

Product

Resources

About