Evaluation of the LIAISON XL Zika Capture IgM II for the Diagnosis of Zika Virus Infections

Abstract

The aim of this study is to evaluate the performance characteristics of the LIAISON XL Zika Capture IgM II. For this purpose we tested 128 samples obtained from recent infections caused by the Zika (ZIKV; 74 samples), dengue (DENV; 10 samples), chikungunya (CHIK V; 11 samples), rubella (RUBV; 10 samples) and measles (MeV; 10 samples) viruses, as well as human parvovirus B19 (HPVB19; 13 samples). The results of the assay under evaluation are compared with those obtained from an indirect immunofluorescence (IIF) assay, and the discrepancies are resolved by considering other laboratory results (PCR and a plaque-reduction neutralization test). The LIAISON showed excellent sensitivity (100%). The specificity (91.25%) was hampered by some false-positive results in recent dengue virus, chikungunya virus, measles virus and human parvovirus B19 infections. The method evaluated is adequate, but the low specificity makes it necessary to consider the clinical and epidemiological contexts of patients, as well as other laboratory results.

Special Issue “Emerging Viruses: Surveillance, Prevention, Evolution, and Control”

“…Arbovirus diagnosis, in particular Zika virus diagnosis, is also discussed. Pérez-Olmeda [17] evaluates the performance characteristics of the LIAISON XL Zika Capture IgM II. Versiane et al [18] describe the identification of B-cell epitopes with potential use to discriminate dengue from zika infections in serological assays.…”