Evaluation of the liver protection and toxicity of Da-Huang-Zhe-Chong pill in rats

Xing, Xiaoyan; Yan, Zhao; Jia, Lei; Wei-jun, Kong; Zhong, Yanwei; Wang, Jiabo; Zhang, Ping; Ren, Huiling; Xiao, Xiaohe

doi:10.3109/13880209.2011.604333

Cited by 11 publications

(11 citation statements)

References 9 publications

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“…DHZCP is a TCM ancient formula from "Jin Kui Yao Lue", authorized by Zhang Zhongjing, The components in DHZCP had the effect of promoting blood circulation to removing blood stasis and strengthening the body resistance, which in accordance with the clinical pathogenesis of hepatic brosis [23,24]. In our previous study, we used CCl4 to induced liver brosis model and found that DHZCP could alleviate liver brosis and improve the level of serum markers of liver function(alanine aminotransferase, aspartate aminotransferase, TGF-β1 and LPS) [25].…”

Section: Discussionmentioning

confidence: 99%

WITHDRAWN: The extract of a Traditional Chinese Medicine alleviates Hepatic Fibrosis of Rats by inhibiting NF-κB activation and upregulating BAMBI in Hepatic Stellate Cells

Li¹,

Tang²,

Lv³

et al. 2020

Preprint

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Background DaHuangZheChong pill (DHZCP) is a formula of traditional Chinese medicine, which has been written into the guideline for the prevention and treatment of hepatic fibrosis in China. The study was aim to investigate the anti-fibrotic effects and the potential mechanisms of DHZCP revolving around the TGF-β pseudo receptor, bone morphogenic protein and activin membrane-bound inhibitor (BAMBI) in hepatic stellate cells (HSCs). Materials and Methods Wistar rats were given with CCL4 for four weeks to establish hepatic fibrosis model. Then the rats were given normal saline or DHZCP decoction six weeks. the pathology of liver tissue was analyzed, the expression of Toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), BAMBI, and NF-κB were detected. In vitro, the associated signal molecules about LPS-activated NF-κB were also analyzed by immunohistochemistry, western blot, or electrophoretic mobility shift assay (EMSA) in cultured HSC-T6 cells . Results The DHZCP showed significant effects on improving fibrosis stage of liver tissue and inhibiting primary HSCs activation. The protein expression of TLR4/MyD88 was lower (P was both < 0.05), BAMBI was higher in DHZCP group than model control (MC) group (P < 0.05) in primary HSCs. In HSC-T6 cells, the activity of NF-κB was lower (P < 0.001), and BAMBI was higher (P < 0.05) in DHZCP added LPS group than in LPS group. Conclusion These results suggested that DHZCP alleviates hepatic fibrosis that was maybe associated with inhibiting activation of NF-κB induced by LPS, and upregulating BAMBI expression in HSCs.

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Section: Discussionmentioning

confidence: 99%

WITHDRAWN: The extract of a Traditional Chinese Medicine alleviates Hepatic Fibrosis of Rats by inhibiting NF-κB activation and upregulating BAMBI in Hepatic Stellate Cells

Li¹,

Tang²,

Lv³

et al. 2020

Preprint

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“…11,13,19 In addition, preclinical studies showed that administration of DHZC pill exerted hepatoprotective activity in carbon tetrachloride-induced liver injury rats. 39 DHZC inhibited hepatic stellate cells proliferation and secretion of transforming growth factor-beta 1, 40 down-regulated p38 mitogen activated protein kinase and extracellular signalregulated kinase pathway, 41 up-regulated gene expression of matrix metalloproteinase (MMP)-1 and increased the activity of MMP-2 in hepatic stellate cells. 42 Gastrointestinal reactions (diarrhea and abdominal distension) were the most frequent undesirable adverse effects related to DHZC.…”

Section: Discussionmentioning

confidence: 99%

Effect of Dahuang zhechong formula on liver fibrosis in patients with chronic hepatitis B: A meta-analysis

Wei

Lang

Gong

et al. 2015

Complementary Therapies in Medicine

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“…Myrianthic acid and tomentic acid, two triterpene acid had the highest observed antioxidant activities (Biapa et al, 2007). Chinese herbals have been attracting more attention in recent years because of complementary therapeutic effects to western medicines, and many essential problems that have not yet been solved by conventional medicinal practices (Xing et al, 2012). The presence of active phytochemical substances for treating various forms of hepatitis may provide a substantial basis for the use of these plants in ethnomedicine.…”

Section: Discussionmentioning

confidence: 99%

Hepatoprotection of 1β-hydroxyeuscaphic acid – the major constituent fromRubus aleaefoliusagainst CCl₄-induced injury in hepatocytes cells

Zhao

Chen

et al. 2013

Pharmaceutical Biology

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Hong (2013) Hepatoprotection of 1β-hydroxyeuscaphic acid -the major constituent from Rubus aleaefolius against CCl 4 -induced injury in hepatocytes cells, Pharmaceutical Biology, 51:6, 686-690, DOI: 10.3109/13880209.2012 caused apoptosis to cells but did not induce lipid peroxidation. Following treatment with 1b-hydroxyeuscaphic acid at doses ranging from 1 to 100 mg/mL for 24 h, cellular morphology, cell growth function (MTT assay), ALT, AST, malondialdehyde (MDA) and superoxide dismutase (SOD) were assessed and evaluated under control and exposed conditions. Results: The IC 50 of 1b-hydroxyeuscaphic acid was 15 mg/mL. Exposure of injured BRL-3A at 20 mg/mL changed abnormal size, cellular shrinkage, and enhanced regulation. ALT, AST, MDA enzyme levels were reduced and SOD activity was increased. Discussion and conclusion: Treatment with 1b-hydroxyeuscaphic acid has significant hepatoprotective activity by lowering the leakage of intracellular enzymes, reducing the oxidation of proteins and decreasing the incidence of apoptosis. These results provide a basis for confirming the traditional uses of R. aleaefolius in treating hepatic diseases.Keywords 1b-hydroxyeuscaphic acid, BRL-3A rat liver cell, hepatoprotective History

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Evaluation of the liver protection and toxicity of Da-Huang-Zhe-Chong pill in rats

Cited by 11 publications

References 9 publications

WITHDRAWN: The extract of a Traditional Chinese Medicine alleviates Hepatic Fibrosis of Rats by inhibiting NF-κB activation and upregulating BAMBI in Hepatic Stellate Cells

WITHDRAWN: The extract of a Traditional Chinese Medicine alleviates Hepatic Fibrosis of Rats by inhibiting NF-κB activation and upregulating BAMBI in Hepatic Stellate Cells

Effect of Dahuang zhechong formula on liver fibrosis in patients with chronic hepatitis B: A meta-analysis

Hepatoprotection of 1β-hydroxyeuscaphic acid – the major constituent fromRubus aleaefoliusagainst CCl₄-induced injury in hepatocytes cells

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Evaluation of the liver protection and toxicity of Da-Huang-Zhe-Chong pill in rats

Cited by 11 publications

References 9 publications

WITHDRAWN: The extract of a Traditional Chinese Medicine alleviates Hepatic Fibrosis of Rats by inhibiting NF-κB activation and upregulating BAMBI in Hepatic Stellate Cells

WITHDRAWN: The extract of a Traditional Chinese Medicine alleviates Hepatic Fibrosis of Rats by inhibiting NF-κB activation and upregulating BAMBI in Hepatic Stellate Cells

Effect of Dahuang zhechong formula on liver fibrosis in patients with chronic hepatitis B: A meta-analysis

Hepatoprotection of 1β-hydroxyeuscaphic acid – the major constituent fromRubus aleaefoliusagainst CCl4-induced injury in hepatocytes cells

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Hepatoprotection of 1β-hydroxyeuscaphic acid – the major constituent fromRubus aleaefoliusagainst CCl₄-induced injury in hepatocytes cells