Evaluation of the loop-mediated isothermal amplification assay for Staphylococcus aureus detection: a systematic review and meta-analysis

Abstract

Background Staphylococcus aureus can cause many diseases and even death. It’s important to detect Staphylococcus aureus rapidly and reliably. The accuracy of a novel test named LAMP in detecting Staphylococcus aureus is unclear. Therefore, a systematic review and meta-analysis were conducted to evaluate the accuracy of the LAMP assay for Staphylococcus aureus detection. Methods Four databases were searched for relevant studies. Meta-DiSc 1.4.0 and Stata 12.0 were used for statistical analysis. At the same time, we used QUADAS-2 to assess the studies we included. Two groups of subgroup analysis were done to differentiate the diagnostic effects of various LAMP tests and in cases of different gold standards. Results 11 studies were identified and 19 2 × 2 contingency tables were extracted in our study. The results showed that both pooled sensitivity and specificity of the LAMP assay were 99% (95% CI 99–100). Conclusion The LAMP assay demonstrated high sensitivity and specificity in diagnosing Staphylococcus aureus.

Rapid detection of Staphylococcus aureus using a novel multienzyme isothermal rapid amplification technique

“…Staphylococcus aureus is one of the most common pathogens in clinics causing various infections ( Van Wamel, 2017 ; Oliveira et al, 2018 ), such as enteritis, pneumonia, endocarditis, and bacteraemia ( Parker and Prince, 2013 ; Shorr et al, 2015 ; Kimmig et al, 2021 ). With strong virulence, invasiveness, and antibiotic resistance, S. aureus has become the primary pathogen of hospital and community-acquired infections ( Long et al, 2022 ). Furthermore, S. aureus resistance has gradually increased because of the long detection time and antibiotic misuse ( Mu et al, 2016 ; Shrestha et al, 2019 ).…”

“…The current standard clinical methods for the isolation and identification of S. aureus depend on culture and microscopy ( Zhao et al, 2016 ). Although these methods are stable, inexpensive, and can give both qualitative and quantitative information on the number and the nature of micro-organisms present ( Long et al, 2022 ), they are time-consuming and the complicated operation, thus not suitable for timely diagnosis and treatment. The process, from sample collection, selective culture, and biochemical screening and serological confirmation, takes about 2 to 5 days.…”

Diagnosis of Subclinical Staphylococcal Aureus Bovine Mastitis Using Nanotechnology-Based Techniques

“…Both sensitivity and specificity reached 100%. FLB is used to analyze LAMP diagnosis results [80]. Multiplex loop-mediated isothermal amplification combined with nanoparticles-based lateral flow biosensor (m-LAMP-LFB) is a new diagnostic method that is fast, reliable, and simple to use to identify S. aureus and MRSA from methicillinsusceptible S. aureus (MSSA) [81].…”

Molecular Detection of Methicillin-resistant Staphylococcus aureus Isolated From Foods in Germany Using LAMP Assay

“…Culture and microscopy are essential components of the existing standard clinical protocols for isolating and identifying S. aureus [12 -16]. Despite being reliable, affordable, and capable of providing data on the quantity and kind of microorganism [13,14], but these technologies are time-consuming and difficult to use, making them unsuitable for rapid diagnosis and therapy. It takes between two and five days to complete the process, the process includes collecting samples, culturing selectively, screening biochemically, and confirming serologically.…”