2022
DOI: 10.56557/upjoz/2022/v43i123061
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EVALUATION OF THE MATURATION TIME OF ABATTOIR-SOURCED WATER BUFFALO (Bubalus bubalis) OOCYTES FERTILIZED In vitro

Abstract: The purpose of this study was to compare the maturation, cellular division, and blastocyst production rates of water buffalo oocytes at 18, 21 and 24 hours of incubation. These oocytes were obtained through follicular puncture, assessed, and matured in BO-IVM medium at 38.5°C, and with a 5% of CO2, at 18 (n=31), 21 (n=34) and 24 hours (n=33). Fertilization was done in a BO-IVF medium at 38.5°C, and with a 5% of CO2, for 18 hours; the maturation rate was assessed a day after fertilization. The embryos were cult… Show more

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