Evaluation of the morpho-physiological traits and the genetic diversity of some Egyptian bread wheat cultivars under salt stress conditions

Abulela, Hamiss A.; Shafee, Ezz El; Farag, Hend; Yacoub, I. H.; Elarabi, Nagwa I.

doi:10.1007/s42976-022-00263-4

Cited by 7 publications

(4 citation statements)

References 54 publications

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“…The dendrogram separated the genotypes into three main groups and two minor groupings. Also, Abulela et al [22] studied the genetic diversity between ten Egyptian bread wheat cultivars by SCoT markers and reported that SCoT marker generate cultivar specific markers with 23% which were similar to these results.…”

Section: Scot Analysismentioning

confidence: 55%

“…The start codon targeted polymorphism (SCoT) method, which uses a single primer to anneal to the flanking regions of the translation initiation codon (ATG) on both DNA strands, is a novel, straightforward, and trustworthy gene-targeted marker system [16]. SCoT markers with high polymorphism and high effectiveness have been successfully applied in oak [18], ramie [19], Dendrobium [20], durum wheat [21], and bread wheat [22]. On other hand, foliar application of Zn had a positive effect on wheat grain yield [23] and improved Zn content of both the entire grain and endosperm while soil applications of Zn were not so effective [24], its efficiency depends on many factors like the time of foliar Zn application [25].…”

Section: Introductionmentioning

confidence: 99%

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Zinc accumulation in wheat, and how it's affected by genetics and sulphate of zinc.

et al. 2023

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The significant prevalence of zinc insufficiency in people makes it crucial to increase zinc (Zn) content in wheat grains on a worldwide scale. Grain-based diets high in zinc help combat the global issue of zinc deficiency. Inter Simple Sequence Repeats (ISSR) and Start Codon Targeted Polymorphism (SCoT) were used to analyes the genetic diversity of 10 Egyptian wheat cultivars in order to find the ones with the highest levels of built-in Zn bio-fortification. The level of polymorphism detected by SCoT was greater than that detected by ISSR. To clarify the influence of Zn foliar treatment on wheat grain production in 2019 and 2020, under conditions of zinc deficiency, Zn foliar application (5 g/L as ZnSO 4 .7H 2 O) was administered at two physiological phases (tillering and milking). Foliar treatment greatly raised the zinc content of the granules. A grain production boost of 50% was seen with a Zn content of 55 ppm. Zinc was bio-fortified in the Giza-168, Gemiza-7, and Gemiza-10 cultivars. This means that foliar treatment of ZnSO 4 may be a useful and cost-effective method for achieving agricultural bio-fortification.

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Section: Scot Analysismentioning

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Zinc accumulation in wheat, and how it's affected by genetics and sulphate of zinc.

et al. 2023

View full text Add to dashboard Cite

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“…ISSR is highly polymorphic and essential in research on developmental, hereditary processes, biodiversity, and genome mapping; this PCR-based method can address some of the drawbacks of existing marker techniques, including the expensive nature of AFLP and the poor reproducibility of RAPD, and is applied to a wide variety of plant species ( Alotaibi and Abd-Elgawad, 2022 ). In this sense, the SCoT marker, which directs the coding region of the plant genome by focusing on the sequence around the ATG codon, has gained increasing popularity and is considered one of the critical molecular markers Jedrzejczyk (2020) ; it has proven to be more efficient compared to other random markers caused of their high annealing temperatures and longer primer lengths and designing analyses of it does not necessitate extensive knowledge of the genome sequence ( Abulela et al., 2022 ). In order to ascertain the genetic diversity of J. procera (Arar) plants from various Saudi Arabian locations, ISSR and SCoT markers were used.…”

Section: Introductionmentioning

confidence: 99%

Comparing genetic differentiation and variation using ISSR and SCoT among Juniper plant markers in Saudi Arabia

Al-Yasi,

Al-Qthanin

2024

Front. Plant Sci.

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Juniperus, a genus of fragrant evergreen trees in the Cupressaceae family, encompasses up to 67 distinct species distributed globally. Among these, Juniperus procera, also known as the East African pencil cedar or African Juniper, stands out for its notable medicinal properties. Due to the well-recognized therapeutic benefits of Juniper species, assessing genetic diversity is essential for various breeding initiatives. Thus, in this work, six ISSR and six SCoT primers were utilized to evaluate the genetic diversity between 23 Juniper genotypes collected from different locations in Saudi Arabia. 29 out of 103 and 26 out of 105 amplified bands, respectively, were found to be polymorphic markers using the ISSR and SCoT studies. With the help of 120 genotype-specific markers, including 60 for ISSR and 60 for SCoT, several varieties of Juniper were discovered. In addition, the polymorphism information content (PIC) was computed to assess the effectiveness of the markers. The findings of this study highlight the importance of conserving the genetic diversity of Juniperus procera, as it holds immense potential for developing new medicinal products. Additionally, the results provide valuable insights into the genetic structure of Juniper populations in western Saudi Arabia, which can inform future conservation and management efforts. However, all of the techniques utilized to profile the genotypes of Juniper can be regarded as useful techniques for long-term fingerprinting and diagnostic markers.

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“…Fortunately, molecular markers have helped identify the genes responsible for the complex agro-morpho-physiological traits that confer salt tolerance in several field crops. This has resulted in a more efficient and costeffective evaluation process [14,15,30]. Therefore, in order to enhance the precision of salt tolerance evaluation in genotypes, it is crucial to integrate phenotypic assessment based on agro-morpho-physiological traits with molecular markers to identify the candidate genes involved in the variation of these traits.…”

Section: Introductionmentioning

confidence: 99%

Integrating Agro-Morpho-Physiological Traits and SSR Markers for Detecting the Salt Tolerance of Advanced Spring Wheat Lines under Field Conditions

Junaid,

El-Hendawy,

Al-Ashkar

et al. 2023

Agriculture

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To successfully enhance the salt tolerance of genotypes, it is crucial to conduct field-based trials, establish effective screening criteria and analysis tools, evaluate salt tolerance at various growth stages, and integrate phenotypic assessment-based traits with molecular markers. This study aimed to assess the salt tolerance of 16 F8 recombinant inbred lines (RILs) and eight genotypes by analyzing 13 agro-morpho-physiological traits using various analysis tools and SSR markers under both control and high salinity levels (15 dS m−1) in real field conditions. Analysis of variance (ANOVA), comparison of mean values, calculation of reduction percentage, and multivariate analysis were used to compare the assessed traits among genotypes and identify which traits are the most effective ones in describing the salt tolerance of these genotypes. A heatmap cluster analysis (HMCA) was also employed to categorize the salt tolerance of genotypes into different clusters based on the stress tolerance index (STI) for all traits. The ANOVA results revealed significant statistical differences (p ≤ 0.05) between the genotypes and salinity levels for all assessed traits in each season and their combined data. Moreover, the 150 mM NaCl treatment led to decreases in the assessed traits by 10.2% to 36.9% when compared to the control treatments. Furthermore, the mean values of assessed traits for certain genotypes were approximately one to three times greater than those of other genotypes. Principal component analysis has identified plant dry weight, green leaf area, leaf area index, and grain yield per hectare as effective screening criteria for explaining the substantial variation observed among the genotypes. The HMCA successfully grouped genotypes into three distinct clusters and distinguished the salt-tolerant genotypes from the salt-sensitive and intermediate ones. The 24 genotypes/RILs were classified into three main groups according to the allelic data of 40 SSRs associated with salt-tolerant genes. A weak yet significant correlation was observed between the similarity coefficients of agro-morpho-physiological traits and SSR markers, as determined by the Mantel test (r = 0.13, p < 0.03, and alpha = 0.05). In conclusion, this study has successfully identified several traits, particularly those associated with SSR markers, that greatly contribute to our understanding of the phenotypic and genotypic basis influencing the salt tolerance of wheat genotypes in real field conditions. Consequently, assessing these traits for a large number of wheat plant materials in a rapid and cost-effective manner will be greatly importance in breeding programs aimed at improving salt stress tolerance in this vital food crop. This will be the main focus of our forthcoming research.

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Evaluation of the morpho-physiological traits and the genetic diversity of some Egyptian bread wheat cultivars under salt stress conditions

Cited by 7 publications

References 54 publications

Zinc accumulation in wheat, and how it's affected by genetics and sulphate of zinc.

Zinc accumulation in wheat, and how it's affected by genetics and sulphate of zinc.

Comparing genetic differentiation and variation using ISSR and SCoT among Juniper plant markers in Saudi Arabia

Integrating Agro-Morpho-Physiological Traits and SSR Markers for Detecting the Salt Tolerance of Advanced Spring Wheat Lines under Field Conditions

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Evaluation of the morpho-physiological traits and the genetic diversity of some Egyptian bread wheat cultivars under salt stress conditions

Cited by 7 publications

References 54 publications

Zinc accumulation in wheat, and how it&#039;s affected by genetics and sulphate of zinc.

Zinc accumulation in wheat, and how it&#039;s affected by genetics and sulphate of zinc.

Comparing genetic differentiation and variation using ISSR and SCoT among Juniper plant markers in Saudi Arabia

Integrating Agro-Morpho-Physiological Traits and SSR Markers for Detecting the Salt Tolerance of Advanced Spring Wheat Lines under Field Conditions

Contact Info

Product

Resources

About

Zinc accumulation in wheat, and how it's affected by genetics and sulphate of zinc.

Zinc accumulation in wheat, and how it's affected by genetics and sulphate of zinc.