Evaluation of the mutagenic potential of bacterial polychlorinated biphenyl biodegradation products

Sayler, Gary S.; Reid, Michael; Perkins, B. K.; Pagni, Richard M.; Smith, R. L.; Rao, T.K.; Epler, J.L.; Morrison, William D; DuFrain, R.J.

doi:10.1007/bf01056365

Cited by 13 publications

(4 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A tier approach to determine the respiratory allergenicity potential of low molecular weight chemicals, such as the microbial product alcalase has been described (11). Because co-exposure to microbial agents and their metabolites during bioremediation can occur, the genotoxicity of bioremediation processes has been evaluated (25)(26)(27)(28)(29).…”

Section: Resultsmentioning

confidence: 99%

Survival of Environmental Microbial Agents in CD-1 Mice Following Oral Exposure

George

2000

Microbial Ecology in Health and Disease

View full text Add to dashboard Cite

Microbial agents have many applications in the environment and are replacing chemicals and engineering processes due to improved efficacy, reduced toxicity, and less cost. Typically, innocuous species are used, however, unless the products include genetically engineered microorganisms, potential health effects are not scrutinized. In order to investigate possible health concerns, four surrogate microbial agents were studied in 6i6o and in 6itro. Male CD-1 mice were treated perorally (p.o.) with average doses of 2.62 × 10 8 colony forming units (CFU) of Pseudomonas aureofaciens, 1.16 × 10 8 CFU of Burkholderia cepacia, 1.78× 10 8 CFU of P. fluorescens, or 1.86 ×10 8 CFU of P. putida. To determine clearance of the dosed strains from mice, lungs, intestinal tract (small, large, cecum), mesenteric lymph nodes (MLN), spleen, and liver were homogenized individually, and dilutions inoculated onto selective media for enumeration of intestinal populations. All strains were recovered in the intestinal tract 3 hours after treatment but P. fluorescens and P. aureofaciens were cleared after the first day. P. putida and B. cepacia survived for at least 2 days in the large intestine and B. cepacia was detected in the small intestine 7 days after treatment. P. aureofaciens and B. cepacia were isolated from the lungs for 2 and 7 days. All strains translocated to the MLN and were detected 3 hours after treatment. B. cepacia was isolated from the spleen and liver 3 hours post-treatment. P. aureofaciens and P. putida translocated to the liver within 3 hours of treatment and P. fluorescens translocated to the spleen. None of the dosed strains were detected extraintestinally after 3 hours. All strains were eliminated from in 6itro intestinal continuous cultures. The models described in this study facilitate the identification of potential human safety issues associated with biotechnology agents.

show abstract

Section: Resultsmentioning

confidence: 99%

Survival of Environmental Microbial Agents in CD-1 Mice Following Oral Exposure

George

2000

Microbial Ecology in Health and Disease

View full text Add to dashboard Cite

show abstract

“…Sequence similarity between Pseudononas dihydrodiol dehydrogenase, part of the gene cluster that metabolizes polychlorinated biphenyls, and dehydrogenases involved in metabolism of ribitol and glucitol and synthesis of antibiotics and 17fl-oestradiol, testosterone and corficosterone There has been intense interest in characterizing the gene clusters found in Pseudomonas pseudoalcaligenes and Pseudomonas putida because they enable these organisms to metabolize completely various aromatic hydrocarbons such as biphenyl, polychlorinated biphenyl (PCB), toluene, and naphthalene [1][2][3][4] or convert them to less toxic forms that can be safely dispersed in the environment [5,6]. The genes for these enzymes have been cloned from P. pseudoalcaligenes (1] and P.…”

mentioning

confidence: 99%

Sequence similarity between Pseudomonas dihydrodiol dehydrogenase, part of the gene cluster that metabolizes polychlorinated biphenyls, and dehydrogenases involved in metabolism of ribitol and glucitol and synthesis of antibiotics and 17 β-oestradiol, testosterone and corticosterone

Baker

1990

Biochemical Journal

View full text Add to dashboard Cite

“…PCBs are worldwide environmental pollutants of anthropogenic origin (10). Owing to possible toxic, mutagenic, and carcinogenic effects and proven bioaccumulation properties, they are considered to be a health hazard for humans (9,15,17). The stability of the benzene rings and carbon-halogen bonds makes PCBs extremely resistant to chemical degradation (10).…”

mentioning

confidence: 99%

Characteristics and restriction analysis of the 4-chlorobiphenyl catabolic plasmid, pSS50

Hooper

Dockendorff

Sayler

1989

Appl Environ Microbiol

View full text Add to dashboard Cite

The plasmid pSS50 is a 53-kilobase self-transmissible plasmid of broad host range that has been isolated from several Alcaligenes and Acinetobacter species. This plasmid has previously been shown to mediate the mineralization of 4-chlorobiphenyl to carbon dioxide and water. Physical characterization of this plasmid by restriction analysis indicates that most hexanucleotide cleavage sites are clustered in a 5-kilobase region, leaving large regions without restriction sites. The paucity of restriction sites is not due to DNA.methylation.

show abstract

Evaluation of the mutagenic potential of bacterial polychlorinated biphenyl biodegradation products

Cited by 13 publications

References 19 publications

Survival of Environmental Microbial Agents in CD-1 Mice Following Oral Exposure

Survival of Environmental Microbial Agents in CD-1 Mice Following Oral Exposure

Sequence similarity between Pseudomonas dihydrodiol dehydrogenase, part of the gene cluster that metabolizes polychlorinated biphenyls, and dehydrogenases involved in metabolism of ribitol and glucitol and synthesis of antibiotics and 17 β-oestradiol, testosterone and corticosterone

Characteristics and restriction analysis of the 4-chlorobiphenyl catabolic plasmid, pSS50

Contact Info

Product

Resources

About