Evaluation of the Nano-TiO2 as a Novel Deswelling Material

Chu, Ming; Hou, Yue‐Long; Xu, Liping; Chu, Zhi‐gang; Zhang, Mingbo; Wang, Yuedan

doi:10.3390/molecules21010057

Cited by 5 publications

(6 citation statements)

References 30 publications

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“…Berberine (Sigma-aldrich, St. Louis, MO, USA) was dissolved in deionized water and filtered through a 0.22 μm Millipore filter (Sartorius Co., NY, USA) 40 41 42 . Levofloxacin (LEV) was obtained from Yangzhijiang Pharmaceutical Co. (Taizhou, China).…”

Section: Methodsmentioning

confidence: 99%

Role of Berberine in the Treatment of Methicillin-Resistant Staphylococcus aureus Infections

Chu

Zhang

Liu

et al. 2016

Sci Rep

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Berberine is an isoquinoline alkaloid widely used in the treatment of microbial infections. Recent studies have shown that berberine can enhance the inhibitory efficacy of antibiotics against clinical multi-drug resistant isolates of methicillin-resistant Staphylococcus aureus (MRSA). However, the underlying mechanisms are poorly understood. Here, we demonstrated that sub-minimum inhibitory concentrations (MICs) of berberine exhibited no bactericidal activity against MRSA, but affected MRSA biofilm development in a dose dependent manner within the concentration ranging from 1 to 64 μg/mL. Further study indicated that berberine inhibited MRSA amyloid fibrils formation, which consist of phenol-soluble modulins (PSMs). Molecular dynamics simulation revealed that berberine could bind with the phenyl ring of Phe19 in PSMα2 through hydrophobic interaction. Collectively, berberine can inhibit MRSA biofilm formation via affecting PSMs’ aggregation into amyloid fibrils, and thereby enhance bactericidal activity of antibiotics. These findings will provide new insights into the multiple pharmacological properties of berberine in the treatment of microbial-generated amyloid involved diseases.

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Section: Methodsmentioning

confidence: 99%

Role of Berberine in the Treatment of Methicillin-Resistant Staphylococcus aureus Infections

Chu

Zhang

Liu

et al. 2016

Sci Rep

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“…This in turn reduces the amount of space available for the absorbed liquid, and the higher swelling properties of the Cs/TiO 2 nanocomposite films compared with chitosan only which may be caused by the hydrophilic nature of both TiO 2 and chitosan. Designing effective interventions and treatments for edema‐related diseases; TiO 2 NPs are available to hitch and heal edema in skin wounds (Chu et al, 2016). Additionally, as the interaction of nanoparticles with chitosan increased and the interaction of water molecules with chitosan decreased, TiO 2 NPs happened after their modification by functional groups of oleic acid (CO, NH and OH) (Kubacka et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…TiO 2 NPs are available to hitch and heal edema in skin wounds (Chu et al, 2016). Additionally, as the interaction of nanoparticles with chitosan increased and the interaction of water molecules with chitosan decreased, TiO 2 NPs happened after their modification by functional groups of oleic acid (CO, NH and OH) (Kubacka et al, 2014).…”

Section: Wound Contraction Was Significantly Higher and Gradually Incmentioning

confidence: 99%

Biological applications study of bio‐nanocomposites based on chitosan/TiO₂ nanoparticles polymeric films modified by oleic acid

Hanafy

Desoky

Hussein

et al. 2020

J Biomedical Materials Res

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The aim of the present study was to prepare and characterize nanocomposite films to improve the treatment of skin wounds by applying the film as a bandage. To modify chitosan (Cs) and to prepare nanocomposites, a mixture between titanium dioxide nanoparticles (TiO 2 NPs) was performed at different concentrations (2, 5, 10 and 15 wt%) and oleic acid (OA). The thin nanocomposite films were prepared by using casting method. The prepared films (Cs, Cs/TiO 2 NPs, Cs/OA and Cs/OA/TiO 2 NPs) were described by water absorption (swelling study) and biological degradation. Physico-chemical characterizations of Cs, Cs/OA, Cs/TiO 2 NPs and Cs/OA/TiO 2 NPs (with only 15 wt% TiO 2 NPs) films were determined by X-ray diffraction, transmission high-resolution electron microscopy, field emission scanning electron microscopy, thermal analysis and Fourier transform infrared spectroscopy as well as their mechanical properties. Antimicrobial activity against microorganisms has been studied to assess activity against bacteria. The prepared nanocomposite films showed good antimicrobial activity for both Gram-positive and Gram-negative bacteria. The therapeutic effects of Cs-TiO 2 NPs-oleic acid nanocomposites on healing excision wounds were studied in rat animal model. The data obtained revealed that groups treated with nanocomposites showed enhancement wound closure and speed up wound healing time. K E Y W O R D S antimicrobial activity, chitosan and oleic acid, chitosan and titanium dioxide nanoparticles, nanocomposites films, wound healing 1 | INTRODUCTION Bionanocomposites are beneficial and have been used in a wide range of applications in many fields such as physical, biological, biomedical and pharmaceutical applications (Díez-Pascual, 2019; Loureiro, Azoia, Gomes, & Cavaco-Paulo, 2016; Nikalje, 2015). Nanoparticles are an important component of many classes of biopolymers and bionanocomposites (Lenart & Hore, 2018). Numerous research has stated that TiO 2 nanoparticles at a low content (1-5%) may improve the physical-thermal properties of biocomposite films (Oleyaei, Almasi, Ghanbarzadeh, & Moayedi, 2016; Zhang et al., 2017). Khan and his colleagues reviewed a detailed overview of the properties, and applications of nanoparticles (NPs) present in various forms (Khan, Saeed, & Khan, 2019). Wound dressings develop according to the cause of different wounds. Various types of wound dressings, for example, xerogels, charcoal, alginate, chitosan, and hydrogel, have been introduced for this purpose (Abbasalipour, Mirjalili, Khajavi, & Majidi, 2014).

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“…The normal human hepatocyte cell line L-02 was purchased from the Type Culture Collection of the Chinese Academy of Sciences, Shanghai, China. HepG2 and L-02 cell lines were maintained in Dulbecco’s modified Eagle’s medium(DMEM) with 10% (v/v) fetal bovine serum (FBS), 0.1 mM nonessential amino acids, 100 U/ml penicillin, and 100 μg/ml streptomycin at 37°C in a 5% CO 2 incubator[ 17 ]. Lipofectamine 3000 reagent (Invitrogen) was used for the delivery of siRNA into cells.…”

Section: Methodsmentioning

confidence: 99%

Effects of cancer-testis antigen, TFDP3, on cell cycle regulation and its mechanism in L-02 and HepG2 cell lines in vitro

et al. 2017

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TFDP3, also be known as HCA661, was one of the cancer-testis antigens, which only expressed in human tissues. The recent researches about TFDP3 mostly focused on its ability to control the drug resistance and apoptosis of tumor cells. However, the role of TFDP3 in the progress of the cell cycle is rarely involved. In this study, we examined the expression of TFDP3 in human liver tissues firstly. After that, we detect the expression of TFDP3 at the RNA level and protein level in L-02 cell line and HepG2 cell line, and the location of TFDP3 was defined by immunofluorescence technique. Furthermore, we synchronized the cells to G1 phase, S phase and G2 phase, and arrested cell mitosis. The localization of TFDP3 and co-localization with E2F1 molecules in different phases of hepatocyte lines. Finally, TFDP3 gene knockout was performed on L-02 and HepG2 cell lines, and detected the new cell cycles by flow cytometry. The result showed that the expression of TFDP3 molecule is negative in normal liver tissue, but positive in immortalized human hepatocyte cell line, and the expression level is lower than in hepatocellular carcinoma cell line. The expression level of TFDP3 was in the dynamic change of L-02 and HepG2 cell lines, and was related to the phase transition. TFDP3 can bind to E2F1 molecule to form E2F/TFDP3 complex; and the localizations of TFDP3 and E2F1 molecules and the co-localization were different in different phases of cell cycle in the nucleus and cytoplasm, which indicated that the E2F/TFDP3 complex involved in the process of regulating the cell cycle. By knocking down the TFDP3 expression level in L-02 and HepG2 cell lines, the cell cycle would be arrested in S phase, which confirmed that TFDP3 can be a potential target for tumor therapy.

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Evaluation of the Nano-TiO2 as a Novel Deswelling Material

Cited by 5 publications

References 30 publications

Role of Berberine in the Treatment of Methicillin-Resistant Staphylococcus aureus Infections

Role of Berberine in the Treatment of Methicillin-Resistant Staphylococcus aureus Infections

Biological applications study of bio‐nanocomposites based on chitosan/TiO₂ nanoparticles polymeric films modified by oleic acid

Effects of cancer-testis antigen, TFDP3, on cell cycle regulation and its mechanism in L-02 and HepG2 cell lines in vitro

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Evaluation of the Nano-TiO2 as a Novel Deswelling Material

Cited by 5 publications

References 30 publications

Role of Berberine in the Treatment of Methicillin-Resistant Staphylococcus aureus Infections

Role of Berberine in the Treatment of Methicillin-Resistant Staphylococcus aureus Infections

Biological applications study of bio‐nanocomposites based on chitosan/TiO2 nanoparticles polymeric films modified by oleic acid

Effects of cancer-testis antigen, TFDP3, on cell cycle regulation and its mechanism in L-02 and HepG2 cell lines in vitro

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Product

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Biological applications study of bio‐nanocomposites based on chitosan/TiO₂ nanoparticles polymeric films modified by oleic acid