Evaluation of the novel artus C. difficile QS-RGQ, VanR QS-RGQ and MRSA/SA QS-RGQ assays for the laboratory diagnosis of Clostridium difficile infection (CDI), and for vancomycin-resistant enterococci (VRE) and methicillin-resistant Staphylococcus aureus (MRSA) screening

Morris, K.; Macfarlane-Smith, Louissa; Wilcox, Mark H.

doi:10.1007/s10096-016-2867-6

Cited by 5 publications

(3 citation statements)

References 37 publications

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“…The false positive results of the vanB test are related to the lack of specificity of the primers used in the test [ 15 ] and, above all, the presence of other microorganisms in the test material, having genes with a high level of similarity or identical to Enterococcus vanB [ 17 , 18 ]. In our study, positive results for vanB were in no case confirmed in VRE culture on chromogenic medium – we found no true positive results.…”

Section: Discussionmentioning

confidence: 99%

Incidence of genes encoding vanA/vanB vancomycin resistance in rectal swabs of patients with diagnosed cancer, on the day of admission to hospital, in a non-epidemic period

Szymankiewicz¹,

Wróblewska²,

Nowikiewicz³

2020

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Introduction: Rapid diagnosis is important for preventing infections due to vancomycin-resistant enterococci. Aim: To evaluate the status of gastrointestinal colonisation with strains containing vanA/vanB genes in oncological patients. Material and methods: A total of 167 samples of rectal swabs taken from 161 patients (mean age: 63, range: 29-93 years) were examined, including 113 patients from surgical wards (70.2%) and 48 patients from non-surgical wards (29.8%), with diagnosed cancer. The tests were carried out within 24 h of admitting the patient to the department, using the Cepheid Xpert vanA/vanB test, with a CE marked GeneXpert ® Instrument Systems analyser. Samples with positive vanB gene results were additionally seeded on chromogenic media. Results: The presence of the vanA gene was found in 2.7% and 6.3% of the examined patients, respectively, from the surgical and non-surgical departments, which accounted for 3.7% of all the patients examined. The presence of the vanB gene was detected in 21.1% of the patients, but in no case was there any growth of vancomycin-resistant enterococci on the chromogenic medium. Conclusions: Patients admitted to non-surgical wards were more often colonised with vanA/vanB genes than were patients admitted to surgical wards, but the differences were not statistically significant.

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Section: Discussionmentioning

confidence: 99%

Incidence of genes encoding vanA/vanB vancomycin resistance in rectal swabs of patients with diagnosed cancer, on the day of admission to hospital, in a non-epidemic period

Szymankiewicz¹,

Wróblewska²,

Nowikiewicz³

2020

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“…In addition, the RT-PCR detection results demonstrated that the expression of LasI mRNA, which is a QS system-related gene, can be reduced by the eucalyptus volatile oil. The QS system regulates the secretion, virulence factors (e.g., elastin, exotoxin A, and pyocyanin), and bio lm formation of PA (21). Therefore, the eucalyptus volatile oil has a signi cant effect not only on bacteriostatic but also on the virulence-related system.…”

Section: Discussionmentioning

confidence: 99%

Mechanism of Eucalyptus Volatile Oil Application for Preventing and Treating Pseudomonas Aeruginosa Infection in Vitro

Yang¹,

Huang²,

Huang³

et al. 2020

Preprint

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Background The mechanism of eucalyptus volatile oil application on the prevention and treatment of burn infections caused by Pseudomonas aeruginosa (PA) was need to examined.Methods The effect of eucalyptus volatile oil on bacterial biofilm was investigated using a PA biofilm model. The expression of LasI mRNA in PA was detected by RT-PCR. Results The minimum inhibitory concentration test showed that the volatile oil of Eucalyptus urophylla in 20% concentration or more could exert an antibacterial effect. However, neither high nor low concentration of the eucalyptus volatile oil had a zone of inhibition. In scanning electron microscopy, the volatile oil group exhibited a significant delay compared with the control group. The volatile oil group also had a significantly lower LasI mRNA expression than the control group. Conclusion As a Chinese medicine, the volatile oil of E. urophylla can affect PA proliferation and biofilm formation by interfering with the expression of LasI, thereby successfully preventing and treating burn infection caused by PA.

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“…and bio lm formation of P.a. are regulated by the QS system [17], so we can consider that the Eucalyptus urophylla volatile oil has a signi cant effect not only on bacteriostatic, but also on the virulence-related system. In summary, the Eucalyptus urophylla volatile oil has high application value in the treatment of clinical infections.…”

Section: Anti-bacterial Effect Of Eucalyptus Volatile Oilmentioning

confidence: 99%

Study on the Mechanism of Application of Eucalyptus Volatile oil in Prevention and Treatment of Burn Wound Infections in Vitro

Yang¹,

Huang²,

Gao³

et al. 2020

Preprint

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Objective To investigate working mechanism of Eucalyptus volatile oil on the prevention and treatment of burn wound infections.Methods P.a biofilm model was used to investigate the effect of Eucalyptus volatile oil on bacterial biofilm. The expression of LasI mRNA in P.a was detected by RT-PCR.Results MIC test showed that the volatile oil of Eucalyptus Urophylla in a concentration of 20% or more could exert anti-bacterial effect. However, no zone of inhibition could be observed in the neither high nor low concentration of the Eucalyptus volatile oil. Scanning electron microscopy results showed a significant delay in volatile oil groups when compared with the control group. The expression of LasI mRNA in the volatile oil group was significantly lower than that in the control group. ConclusionAs Chinese medicine, the volatile oil of Eucalyptus Urophylla can affect the proliferation of P.a and biofilm formation by interfering with the expression of LasI, thus achieving the purpose of preventing and treating infection of burn patients.

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Evaluation of the novel artus C. difficile QS-RGQ, VanR QS-RGQ and MRSA/SA QS-RGQ assays for the laboratory diagnosis of Clostridium difficile infection (CDI), and for vancomycin-resistant enterococci (VRE) and methicillin-resistant Staphylococcus aureus (MRSA) screening

Cited by 5 publications

References 37 publications

Incidence of genes encoding vanA/vanB vancomycin resistance in rectal swabs of patients with diagnosed cancer, on the day of admission to hospital, in a non-epidemic period

Incidence of genes encoding vanA/vanB vancomycin resistance in rectal swabs of patients with diagnosed cancer, on the day of admission to hospital, in a non-epidemic period

Mechanism of Eucalyptus Volatile Oil Application for Preventing and Treating Pseudomonas Aeruginosa Infection in Vitro

Study on the Mechanism of Application of Eucalyptus Volatile oil in Prevention and Treatment of Burn Wound Infections in Vitro

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