Evaluation of the on-site immunoassay drug-screening device Triage-TOX in routine forensic autopsy

Tominaga, Mariko; Michiue, Tomomi; Maeda, Hitoshi

doi:10.1016/j.legalmed.2015.10.007

Cited by 13 publications

(7 citation statements)

References 13 publications

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“…Other point-of-care detection kits, such as Monitect-9 R or Triage-Tox R are also subject to the same limitations. 8 There is a definite need for alternative methods of detecting ingested drugs from various human specimens, candidates for which include high performance liquid chromatography (HPLC), gas chromatography/mass spectroscopy, and liquid chromatography/mass spectroscopy (LC/MS). [9][10][11] Liquid chromatography/mass spectroscopy sorts chemicals using HPLC and analyzes them using mass spectroscopy.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, Triage DOA R can analyze only urine samples and cannot be used for gastric lavage or blood samples. Other point‐of‐care detection kits, such as Monitect‐9 R or Triage‐Tox R are also subject to the same limitations . There is a definite need for alternative methods of detecting ingested drugs from various human specimens, candidates for which include high performance liquid chromatography (HPLC), gas chromatography/mass spectroscopy, and liquid chromatography/mass spectroscopy (LC/MS) …”

Section: Introductionmentioning

confidence: 99%

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Diagnostic accuracy for drug detection using liquid chromatography/mass spectroscopy in overdose patients

Yagihashi

Tarui

Miyagi

et al. 2020

Acute Medicine & Surgery

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Information about the causative drugs is essential for appropriate treatment for drug overdose, but patients sometimes cannot provide information about overdosed drugs owing to disturbed consciousness or an unwillingness to cooperate with treatment. The purpose of this study was to decide whether liquid chromatography/mass spectroscopy (LC/MS) is useful as a detection method for overdosed drugs. Methods: Overdose patients (n = 279) treated in our facility were retrospectively studied. Specimens from gastric lavage, blood serum, and urine were tested using LC/MS. The matching rates between drugs overdosed and those detected by LC/MS were evaluated; LC/MS and Triage DOA R were also compared. Data are shown as means. Results: Patients overdosed on 3.2 kinds of drugs and were transferred to our hospital 4.6 h after. Overall 3.5 kinds of drugs were detected by LC/MS, and 2.4, 1.9, and 2.2 kinds were from the stomach, blood, and urine, respectively. Matching rate among the ingested drugs (kinds of drugs matched/ones ingested) was the highest in the gastric samples (0.56), and the lowest in the urine samples (0.46) (P < 0.01). In addition, the matching rates among the detected drugs (kinds of drugs matched/ones detected) were as high as 0.74 and 0.78 in the gastric and blood samples, respectively. Comparing the sensitivity and specificity of detection of benzodiazepines and tricyclic antidepressants between LC/MS and Triage DOA R , we found that these two methods were comparable. Conclusion: Liquid chromatography/mass spectroscopy was proven to be an effective method to detect overdosed drugs, especially when there was not enough information about the drugs ingested.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Diagnostic accuracy for drug detection using liquid chromatography/mass spectroscopy in overdose patients

Yagihashi

Tarui

Miyagi

et al. 2020

Acute Medicine & Surgery

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mentioning

confidence: 99%

“…Triage TOX Drug Screen (Alere Inc., San Diego, CA, USA), a novel, rapid toxicology screening test based on a competitive fluorescence immunoassay, has recently been introduced [ 10 ]. This test provides preliminary qualitative results through one-step processing following specimen application to an automatic analyzer, thus ensuring objectivity by instrumental colorimetric calibration and the subsequent printing of positive or negative results, independent of operator [ 10 ]. This method can detect 11 drugs and/or their major metabolites, including acetaminophen (APAP), amphetamine (AMP), methamphetamine (mAMP), barbiturates (BAR), benzodiazepine (BZO), cocaine (COC), methadone (MTD), opiates (OPI), phencyclidine (PCP), tetrahydrocannabinol (THC), and tricyclic antidepressants (TCA), at concentrations higher than the urine threshold.…”

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confidence: 99%

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Evaluation of the Triage TOX Drug Screen Assay for Detection of 11 Drugs of Abuse and Therapeutic Drugs

2017

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The demand for rapid and broad clinical toxicology screens is on the rise. Recently, a new rapid toxicology screening test, the Triage TOX Drug Screen (Alere Inc., USA), which can simultaneously detect 11 drugs of abuse and therapeutic drugs with an instrument-read cartridge, was developed. In the present study, we evaluated the efficacy of this new on-site immunoassay using 105 urine specimens; the results were compared with those obtained by using ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-TMS). Precision was evaluated according to the CLSI EP12-A2 for analyte concentrations near the cutoff, including C50 and±30% of C50, for each drug using standard materials. The C50 specimens yielded 35–65% positive results and the ±30% concentration range of all evaluated drugs encompassed the C5–C95 interval. The overall percent agreement of the Triage TOX Drug Screen was 92.4–100% compared with UPLC-TMS; however, the Triage TOX Drug Screen results showed some discordant cases including acetaminophen, amphetamine, benzodiazepine, opiates, and tricyclic antidepressants. The overall performance of the Triage TOX Drug Screen assay was comparable to that of UPLC-TMS for screening of drug intoxication in hospitals. This assay could constitute a useful screening method for drugs of abuse and therapeutic drugs in urine.

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Development of an immunoassay for fluvoxamine detection using a recombinant single-chain variable fragment antibody

et al. 2017

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Evaluation of the on-site immunoassay drug-screening device Triage-TOX in routine forensic autopsy

Cited by 13 publications

References 13 publications

Diagnostic accuracy for drug detection using liquid chromatography/mass spectroscopy in overdose patients

Diagnostic accuracy for drug detection using liquid chromatography/mass spectroscopy in overdose patients

Evaluation of the Triage TOX Drug Screen Assay for Detection of 11 Drugs of Abuse and Therapeutic Drugs

Development of an immunoassay for fluvoxamine detection using a recombinant single-chain variable fragment antibody

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