Evaluation of the oncolytic property of recombinant Newcastle disease virus strain R2B in 4T1 and B16-F10 cells in-vitro

Narayan, Raj; Pathak, D. C.; D’Silva, Ajai Lawrence; Batheja, Rahul; Kumar, Manoj; Vakharia, Vikram N.; Chellappa, Madhan Mohan; Dey, Sohini

doi:10.1016/j.rvsc.2021.07.028

Cited by 5 publications

(5 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our study, we observed a significant loss of MMP, time-dependent degradation of Bcl-2 protein, time-dependent increase in Bid cleavage, mitochondrial translocation of Bax, and release of mitochondrial Cyt C following NDV infection. These findings are consistent with previous studies [26,67,68] and clearly demonstrate that NDV infection induces mitochondrial dysfunction, ultimately resulting in mitochondria-dependent apoptosis. Importantly, we discovered that inhibiting either CTSB or CTSD activity effectively mitigates NDVinduced mitochondria-dependent apoptosis, providing further support for the role of LMPinduced translocated cathepsins in mitochondrial dysfunction and subsequent activation of cell apoptosis.…”

Section: Plos Pathogenssupporting

confidence: 93%

The HN protein of Newcastle disease virus induces cell apoptosis through the induction of lysosomal membrane permeabilization

Chen,

Zhu,

Liao

et al. 2024

PLoS Pathog

View full text Add to dashboard Cite

Lysosomes are acidic organelles that mediate the degradation and recycling of cellular waste materials. Damage to lysosomes can cause lysosomal membrane permeabilization (LMP) and trigger different types of cell death, including apoptosis. Newcastle disease virus (NDV) can naturally infect most birds. Additionally, it serves as a promising oncolytic virus known for its effective infection of tumor cells and induction of intensive apoptotic responses. However, the involvement of lysosomes in NDV-induced apoptosis remains poorly understood. Here, we demonstrate that NDV infection profoundly triggers LMP, leading to the translocation of cathepsin B and D and subsequent mitochondria-dependent apoptosis in various tumor and avian cells. Notably, the released cathepsin B and D exacerbate NDV-induced LMP by inducing the generation of reactive oxygen species. Additionally, we uncover that the viral Hemagglutinin neuraminidase (HN) protein induces the deglycosylation and degradation of lysosome-associated membrane protein 1 (LAMP1) and LAMP2 dependent on its sialidase activity, which finally contributes to NDV-induced LMP and cellular apoptosis. Overall, our findings elucidate the role of LMP in NDV-induced cell apoptosis and provide novel insights into the function of HN during NDV-induced LMP, which provide innovative approaches for the development of NDV-based oncolytic agents.

show abstract

Section: Plos Pathogenssupporting

confidence: 93%

The HN protein of Newcastle disease virus induces cell apoptosis through the induction of lysosomal membrane permeabilization

Chen,

Zhu,

Liao

et al. 2024

PLoS Pathog

View full text Add to dashboard Cite

show abstract

“…A classic antitumor mechanism employed by OVs is replication within cancer cells, followed by direct lysis of these cells; this mechanism is termed direct virus-induced oncolysis (Figure 2) [40]. Consequently, transgenes are expressed and trigger host immune responses, thereby activating antitumor immunity [69] It has been reported that an engineered Newcastle disease virus leads to the loss of mitochondrial membrane permeability in tumor cells, followed by ICD [70]. The M1 virus induces endoplasmic reticulum stress-mediated apoptosis, followed by cytolysis of tumor cells [71].…”

Section: Direct Tumor Lysismentioning

confidence: 99%

“…This mechanism induces tumor cell death locally by direct tumor lysis, but it also triggers a systemic response by activating the immune system (Figure 2D) and activates the endothelial cells involved in the vascularization of the tumor, reducing tumor angiogenesis [73]. It has been reported that an engineered Newcastle disease virus leads to the loss of mitochondrial membrane permeability in tumor cells, followed by ICD [70]. The M1 virus induces endoplasmic reticulum stress-mediated apoptosis, followed by cytolysis of tumor cells [71].…”

Section: Direct Tumor Lysismentioning

confidence: 99%

Oncolytic Virotherapy: A New Paradigm in Cancer Immunotherapy

Volovat,

Scripcariu,

Vasilache

et al. 2024

IJMS

View full text Add to dashboard Cite

Oncolytic viruses (OVs) are emerging as potential treatment options for cancer. Natural and genetically engineered viruses exhibit various antitumor mechanisms. OVs act by direct cytolysis, the potentiation of the immune system through antigen release, and the activation of inflammatory responses or indirectly by interference with different types of elements in the tumor microenvironment, modification of energy metabolism in tumor cells, and antiangiogenic action. The action of OVs is pleiotropic, and they show varied interactions with the host and tumor cells. An important impediment in oncolytic virotherapy is the journey of the virus into the tumor cells and the possibility of its binding to different biological and nonbiological vectors. OVs have been demonstrated to eliminate cancer cells that are resistant to standard treatments in many clinical trials for various cancers (melanoma, lung, and hepatic); however, there are several elements of resistance to the action of viruses per se. Therefore, it is necessary to evaluate the combination of OVs with other standard treatment modalities, such as chemotherapy, immunotherapy, targeted therapies, and cellular therapies, to increase the response rate. This review provides a comprehensive update on OVs, their use in oncolytic virotherapy, and the future prospects of this therapy alongside the standard therapies currently used in cancer treatment.

show abstract

“…When an oncolytic virus infects and replicates in tumor cells, it affects the synthesis of nucleic acid and protein in cells and damages organelles such as lysosomes, endoplasmic reticulum, and mitochondria, leading to alterations in cell function and, finally, killing tumor cells (Figure 1). For example, the recombinant Newcastle disease virus R2B-GFP virus causes the loss of mitochondrial membrane permeability in 4T1 and B16-F10 cells, resulting in cell death (Ramamurthy et al, 2021). The M1 virus kills cancer cells by inducing endoplasmic reticulum stress-mediated apoptosis (Lin et al, 2014).…”

Section: Direct Oncolysismentioning

confidence: 99%

Oncolytic Viruses: Immunotherapy Drugs for Gastrointestinal Malignant Tumors

Oduro

Guo

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Oncolytic virus therapy has advanced rapidly in recent years. Natural or transgenic viruses can target tumor cells and inhibit tumor growth and metastasis in various ways without interfering with normal cell and tissue function. Oncolytic viruses have a high level of specificity and are relatively safe. Malignant tumors in the digestive system continue to have a high incidence and mortality rate. Although existing treatment methods have achieved some curative effects, they still require further improvement due to side effects and a lack of specificity. Many studies have shown that oncolytic viruses can kill various tumor cells, including malignant tumors in the digestive system. This review discusses how oncolytic virus therapy improves malignant tumors in the digestive system from the point-of-view of basic and clinical studies. Also, the oncolytic virus anti-tumor mechanisms underpinning the therapeutic potential of oncolytic viruses are expounded. In all, we argue that oncolytic viruses might eventually provide therapeutic solutions to malignant tumors in the digestive system.

show abstract

Evaluation of the oncolytic property of recombinant Newcastle disease virus strain R2B in 4T1 and B16-F10 cells in-vitro

Cited by 5 publications

References 26 publications

The HN protein of Newcastle disease virus induces cell apoptosis through the induction of lysosomal membrane permeabilization

The HN protein of Newcastle disease virus induces cell apoptosis through the induction of lysosomal membrane permeabilization

Oncolytic Virotherapy: A New Paradigm in Cancer Immunotherapy

Oncolytic Viruses: Immunotherapy Drugs for Gastrointestinal Malignant Tumors

Contact Info

Product

Resources

About