Evaluation of the overnight hold of whole blood at room temperature, before component processing: platelets (PLTs) from PLT‐rich plasma

Abstract

In vitro storage conditions produce immediate differences after preparation, which disappear throughout storage. PCs from overnight-held WB have similar in vitro variables as from freshly processed WB. These findings warrant confirmation in clinical trials, but underscore the possibility of use of these PCs as being equivalent to those obtained from freshly processed WB.

The Effect of Holding Times of Whole Blood and Its Components During Processing on In Vitro and In Vivo Quality

“…They saw a slight but significant increase in platelet activation markers (such as CD62P expression, lysosomal integral membrane protein, annexin A5, factor V-Va, bound von Willebrand factor [vWF], and bound fibrinogen) during the holding period, but similar to the findings of Van der Meer et al [31], this dropped to baseline levels once the platelet concentrates were prepared. At onset of storage, no relevant differences were demonstrated.…”

“…No effect (shown for PRP-derived platelets only) Platelet concentration 0-30% higher [4,19,30,32] pH, lactate pH lower, lactate higher, but disappears during storage [6,13,29,31,34,36] HSR HSR initially lower, but disappears during storage [13,31,34,36] Other PLT quality measures…”

“…Minor effects that disappear during storage [13,31,34,36] Leukoreduction Less efficient with older types of filters, but residual white cells within specifications. For currently marketed filters no difference [34], Table 1 Risk of bacterial outgrowth…”

“…Neither platelet counts nor in vitro quality (ATP content, extent of shape change, thrombin response; CD62P expression was comparable initially but marginally lower in favor of the 24-hour hold group on day 8) differed. In a BEST Collaborative study comparing 3-vs 24-hour½ hold, with blood processed according to the PRP method, the platelet concentrates contained 30% more platelets when whole blood was held overnight [31]. No difference in CD62P expression was seen; initially, a lower HSR was observed for 3-hour-held whole blood, but during storage, this difference disappeared.…”

Wissenschaftliche Erläuterungen zur Stellungnahme „Bewertung von Apherese- und Pool-Thrombozytenkonzentraten“ des AK Blut vom 31.03.2015

“…Die aktuelle Datenlage bietet keinen Anhalt dafür, dass eine Raumtemperaturlagerung der Vollblutspenden bis zur PTK-Herstellung einen nachteiligen Einfluss auf die PTK-Qualität hat, solange das Intervall geringer als 24 h ist [14][15][16][17][18][19][20][21] [57,[59][60][61][62].…”