Evaluation of the Ovicidal Activity and Fasciolicidal Activity of the Extract of Ethyl Acetate from Artemisia Ludoviciana Nutt. Spp. Mexicana and of Artemisinin Against Adult Parasites of Fasciola Hepatica

Abstract

The objective of this work was to evaluate the effect of the ethyl acetate extract of A. ludoviciana and artemisinin against adult parasites and eggs of F. hepatica. Extract from A. ludoviciana were obtained by using ethyl acetate. To carry out the ovicidal assay, cell culture boxes were used, and 90 to 110 F. hepatica eggs were placed in each well. The eggs were exposed in triplicate to concentrations of 100, 200, 300, 400 and 500 mg/L of the extract, and they were incubated for 14 and 16 days at a temperature of 28°C and 80% humidity. Adult flukes were exposed to concentrations of 125, 250, 375 and 500 mg/L ethyl acetate extract of A. ludoviciana. Additionally, triclabendazole was used as a reference drug at concentrations of 10 and 50 mg, and at the same time the response of artemisinin at concentrations of 10 and 20 mg was assessed. The results of the percentage of ovicidal action of the eggs exposed to triclabendazole showed 3.23% and 16.13% at 14 days of incubation, 3.89% and 17.03% at 16 days, artemisinin showed 57.75% and 65.95% at 14 days, 59.10% and 68.23% at 16 days. Finally ethyl acetate extract of A. ludoviciana showed 83.55% at 14 days and 93.65% at 16 days (P < 0.05). The fasciolicidal activities of triclabendazole and artemisinin showed 100% of efficacy at 24 hours of exposure. ethyl acetate extract of A. ludoviciana showed 100% of efficacy at 48 and 72 hours of exposure (P < 0.05).