Evaluation of the Oxidative Effect of Long-Term Repetitive Hyperbaric Oxygen Exposures on Different Brain Regions of Rats

Şimşek, Kemal; Özler, Mehmet; Yıldırım, Ali Osman; Sadir, Serdar; Demirbaş, Şeref; Öztosun, Muzaffer; Korkmaz, Ahmet; Ay, Hakan; Öter, Şükrü; Yıldız, Şenol

doi:10.1100/2012/849183

Cited by 19 publications

(15 citation statements)

References 33 publications

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“…Speit and Bonzheim [2003] implicated heme oxygenase-1 in HBO 2 -induced protection of A549 cells against oxygen toxicity induced by a second HBO 2 treatment 24 h later. Recently it was reported that daily exposure to 90 min HBO 2 @ 2.8 ATA for as many as 40 days failed to significantly elevated levels of MDA, protein carbonyl and glutathionine peroxidase in the cerebral cortex, inner white matter and cerebellum [Simsek et al, 2012]. These findings, albeit at a lower atmospheric pressure than that applied in the present study, as well as our results are consistent in demonstrating a lack of oxygen toxicity.…”

Section: Discussionsupporting

confidence: 93%

The acute antinociceptive effect of hyperbaric oxygen is not accompanied by an increase in markers of oxidative stress

2014

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Aims Exposure to hyperbaric oxygen (HBO2) causes an antinociceptive response in mice. However, breathing oxygen (O2) at an elevated pressure can potentially cause oxygen toxicity. The aim of this study was to identify the determinants of HBO2 antinociception and the toxicity profile of HBO2. Main methods Male NIH Swiss mice were assessed for acute antinociceptive responsiveness under room air or 100% O2 at 1.0 or 3.5 atmospheres absolute (ATA), using the acetic acid-induced abdominal constriction test. For the oxygen toxicity test, mice were exposed to 3.5 ATA oxygen for 11 min, 60 min, 60 min daily for 2 days (120 min) or 60 min daily for 4 days (240 min), then assessed by analyzing the levels of two oxidative stress markers, MDA (malondialdehyde) and protein carbonyl in brain, spinal cord and lung. Key Findings Only the combination of 100% O2 and 3.5 ATA caused significant antinociception. The antinociceptive effect of 100% O2 was pressure-dependent up to 3.5 ATA. In the oxygen toxicity test, mice exposed to HBO2 for different time intervals had levels of brain, spinal cord and lung MDA and protein carbonyl that were comparable to that of control animals exposed to room air. Significance Treatment with 100% O2 evokes a pressure-dependent antinociceptive effect. Since there was no significant increase in levels of the oxidative stress markers in the tested tissues, it is concluded HBO2 at 3.5 ATA produces antinociception in the absence of oxidative stress in mice.

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Section: Discussionsupporting

confidence: 93%

The acute antinociceptive effect of hyperbaric oxygen is not accompanied by an increase in markers of oxidative stress

2014

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“…Another experimental set revealed an important finding that the enhanced levels of oxidation products declined to their baseline values at one hour later following a single HBO exposure (13,14). Finally, in our most recent studies, different from the abovementioned one-session HBO exposure procedures, after exposure to daily HBO sessions for up to 8 weeks, clear rises in lipid and protein oxidation products along with the antioxidant enzyme superoxide dismutase (SOD) were detected in the rats' lung (15), but not in their brain tissue (16).…”

Section: Introductionmentioning

confidence: 60%

“…The good news was that increased activities of antioxidant enzymes accompanied the rise of these oxidation products. On the other hand, the brain tissue specimens of rats did not reflect any significant change for oxidant and antioxidant indices in the same experimental set (16). With regard to hyperoxia-induced oxidative injury, the lung tissue is the first target, since it is the entering site of oxygen; then, oxygen passes into the blood and the oxygen-carrier cells, erythrocytes, represent an ideal secondary target for detecting hyperoxic oxidative interactions (9).…”

Section: Discussionmentioning

confidence: 97%

“…With regard to hyperoxia-induced oxidative injury, the lung tissue is the first target, since it is the entering site of oxygen; then, oxygen passes into the blood and the oxygen-carrier cells, erythrocytes, represent an ideal secondary target for detecting hyperoxic oxidative interactions (9). Therefore, in the present work, we focused on the erythrocytes in order to investigate possible similarities and/or differences with the abovementioned lung and brain studies (15,16). As a result, both MDA and PCC levels were detected to increase significantly at earlier time-points than previously seen in the lung (15).…”

Section: Discussionmentioning

confidence: 99%

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Response of Rat Erythrocyte Oxidative Stress Markers to Repetitive Hyperbaric Oxygen Exposures Up to 40 Daily Sessions / Odgovor Markera Oksidativnog Stresa U Eritrocitima Pacova Na Višestruko Izlaganje Hiperbaričnom Kiseoniku Do 40 Seansi Dnevno

Şimşek¹,

Yıldırım²,

Demirbaş³

et al. 2013

Journal of Medical Biochemistry

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SummaryBackground: Studies with single-session hyperbaric oxygen exposures have shown that HBO-induced oxidative stress is proportional to exposure pressure and duration. Since the efficacy of hyperbaric oxygen mainly depends on repetitive exposures, this study aimed to investigate the oxidative effect of hyperbaric oxygen administered for 5 to 40 sessions. Methods: Sixty rats were divided into one control and 6 study groups. Study groups were exposed to 5, 10, 15, 20, 30, and 40 daily consecutive 2.8 atm/90 min hyperbaric oxygen sessions. Animals were sacrificed 24 h after the last hyperbaric oxygen administration. Malondial dehyde and carbonylated protein levels as well as superoxide dismutase activities were determined in isolated rat erythrocytes. Results: Carbonylated protein levels increased significantly after just 5 hyperbaric oxygen exposures; reached a peak level with 10 exposures; were still significantly higher than controls after 15 sessions; and de creased to normal limits after 20 exposures. Malondial dehyde le vels were found to be significantly increased in the 10 to 30, but not in the 5 and 40-session groups. Superoxide dismutase activity showed elevated levels only in the 5 and 10 times hyperbarical oxygenexposed groups. Conclusions: The suppressed oxidative stress level after 40 exposures suggests an effective endogenous antioxidant defense in repetitive HBO administrations.

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“…Nevertheless, ROS have also been suggested to mediate useful actions in relation to cellular functions [6][7][8]. Although there are some experimental studies showing that HBO promotes antioxidant activity by providing more oxygen to the body and thus decreasing the production of ROS and lipid peroxidation, these results have not been validated [9]. Inflammatory response is a broad pathophysiological process.…”

Section: Introductionmentioning

confidence: 99%

Hyperbaric Oxygen Treatment is Associated with Lipid Inflammatory Response Assessed Uding Serum Platelet Activating Factor

Eren¹,

Yıldırım²,

Giray³

et al. 2019

Polish Hyperbaric Research

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Hyperbaric oxygen (HBO) treatment is generally a relatively safe therapy for various conditions. However, there are some adverse side effects. For example HBO tratment has been reported to increase the production of free oxygen radicals(FRs). Furthermore, to our knowledge, no previous clinical research has been carried out to study the involvement of platelet-activating factor(PAF)as the lipid oxidative stressor in patients undergoing HBO treatment. A total of 45 patients included in this study were first given clinical assessment and laboratory measurements before starting HBO treatment and were named group baseline. After the HBO treatment, the same clinical and laboratory measurements from the same patients were repeated and this was named group sesion >20.As expected, long-term HBO treatment had no effect on oxLDL (oxidized low-density lipoprotein), a lipid oxidative stress(OS) marker. However, the mean PAF values in the second group showed a statistically significant increase compared to their pretreatment values, (P <0. 002).As this is a preliminary study, there is a need for more detailed investigations that demonstrate the association of HBO treatment with the lipid inflammatory response. Therefore, there is need for further clinical study for OS markers such as oxLDL in HBO treatment. Clinical prospective studies are required to confirm our laboratory findings.

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Evaluation of the Oxidative Effect of Long-Term Repetitive Hyperbaric Oxygen Exposures on Different Brain Regions of Rats

Cited by 19 publications

References 33 publications

The acute antinociceptive effect of hyperbaric oxygen is not accompanied by an increase in markers of oxidative stress

The acute antinociceptive effect of hyperbaric oxygen is not accompanied by an increase in markers of oxidative stress

Response of Rat Erythrocyte Oxidative Stress Markers to Repetitive Hyperbaric Oxygen Exposures Up to 40 Daily Sessions / Odgovor Markera Oksidativnog Stresa U Eritrocitima Pacova Na Višestruko Izlaganje Hiperbaričnom Kiseoniku Do 40 Seansi Dnevno

Hyperbaric Oxygen Treatment is Associated with Lipid Inflammatory Response Assessed Uding Serum Platelet Activating Factor

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