Evaluation of the Performance Characteristics of an In-House One Step TaqMan Real Time-Polymerase Chain Reaction Assay for Detection and Quantification of Hepatitis C Virus

Kermani, Fahimeh Ranjbar; Kafiabad, S Amini; Hosseini, Kamran Mousavi; Maghsoudlu, Mahtab; Sharifi, Zohreh; Samiee, Shahram

doi:10.5812/jjm.42884

Cited by 2 publications

(1 citation statement)

References 19 publications

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“…Метод основан на включении в бактериальный метаболизм органики, содержащей радиоактивный углерод-14. Впервые радиометрия была применена для определения бактерий Levin и используется в клинической микробиологии, однако, в некоторых случаях она применима и для контроля качества продуктов и питьевой воды [26]. Previte использовал этот метод для определения присутствия Salmonella aureus, Salmonella typhimurium, Clostridium botulinum и спор гнилостного анаэроба 3679 на говядине [36].…”

Section: результаты и их обсуждениеunclassified

Molecular Genetic Methods in Microbiological Control of Food Products

Деревенщикова¹,

Derevshchikova

Сыромятников³

et al. 2019

Food Processing: Techniques and Technology

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There are a number of technologies and business applications that identify nucleic acids of various microorganisms. Technologies based on DNA analysis are the most promising direction in the molecular-genetic identification of the microbiota in food substrates. The present paper is a review of various aspects of microorganism identification in food substrates, their advantages and disadvantages. It features modern regulatory, scientific, and methodological sources, as well as patented solutions. The authors pay considerable attention to the classical methods and describe the use of polymerase chain reaction (PCR) in microbiota analysis. Then, they trace the development of next-generation sequencing (NGS) of DNA and how it can be used to identify pathogens in food substrates. So far, NGS proves to be the most advantageous method that identifies prokaryotic and eukaryotic microorganisms, as well as pathogens.

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Section: результаты и их обсуждениеunclassified

Molecular Genetic Methods in Microbiological Control of Food Products

Деревенщикова¹,

Derevshchikova

Сыромятников³

et al. 2019

Food Processing: Techniques and Technology

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Look-Back Study of Transfusion-Transmitted Hepatitis C Virus Infection in Iran

et al. 2020

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Background: With the identification of blood donors with a subsequent positive result in HCV screening and the possibility of HCV transmission via their previous negative donations, the role of look-back investigation has been crucial in enhancing blood safety. A retrospective study was conducted to identify the fate of blood components from donors subsequently found to be confirmed HCV positive cases with previous negative donations. Objectives: This look-back study aimed to determine the transfusion-induced hepatitis C virus infection through previous HCV-negative donations of subsequent HCV positive blood donors in Iran. Methods: In this study, all serologically confirmed HCV positive blood donors across the country from December 2015 to June 2017 were included. A look-back process was conducted by tracing back previous HCV negative donations of subsequent HCV-positive blood donors to specific recipients, according to the IBTO instructions. The HCV RNA testing was carried out using an in-house one-step TaqMan real-time RT-PCR assay. Results: During the study period, 280 serologically confirmed HCV-positive blood donors were included, with 267 as first-time, nine as repeated, and three as regular donors. Of the participants, the first-time donors were excluded, the repeated donors were not eligible, and only the regular donors were selected for the look-back study. The HCV RNA was detected in one regular donor, but HCV infection was not detected in his previous donation recipient. In addition, more than two HCV risk factors were reported by each regular blood donor. Conclusions: According to the results, evidence of HCV transmission via previous blood transfusions and the risk of HCV infection was not observed among recipients. Due to the reporting of some HCV risk factors by the subjects, paying proper attention to the donor selection process is highly recommended.

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Evaluation of the Performance Characteristics of an In-House One Step TaqMan Real Time-Polymerase Chain Reaction Assay for Detection and Quantification of Hepatitis C Virus

Cited by 2 publications

References 19 publications

Molecular Genetic Methods in Microbiological Control of Food Products

Molecular Genetic Methods in Microbiological Control of Food Products

Look-Back Study of Transfusion-Transmitted Hepatitis C Virus Infection in Iran

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