Evaluation of the Performance of a Multiplex PCR Method for the Diagnosis of Viral Respiratory Tract Infections

Arslan, Ayse Hande; Çiçek, Candan; Saz, Eylem Ulaş; Gülen, Figen; Karakuş, Haydar

doi:10.5222/tmcd.2016.159

Cited by 2 publications

(1 citation statement)

References 7 publications

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“…In this study, total of 3074 patient samples tested was detected 48% positivity. Arslan et al [ 18 ] were detected 47.4% positivity rate and Ecemiş et al [ 19 ] were detected 34.4% positivity. In the pediatric group patients with acute respiratory infection, the rate of detection of respiratory viruses varies between 30 and 96% [ 20 , 21 ].…”

Section: Discussionmentioning

confidence: 99%

Distribution of respiratory viruses: Evaluation of multiplex PCR results of 3074 patients

Zeytinli¹

2021

North Clin Istanbul

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OBJECTIVE Early and accurate diagnosis of acute respiratory infections is important because these diseases negatively affect public health and can lead to loss of workforce and an increase in health expenditures. In this study, we aimed to determine the respiration panel multiplex polymerase chain reaction (PCR) test results and seasonal distribution in our region. METHODS Three thousand and seventy-four patients samples multiplex PCR (Anatolia, Bosphore ® Respiratory Pathogens Panel Kit v1) test results, which were sent to our laboratory, from 13 hospitals in our region between January 2018 and December 2018, were evaluated retrospectively. RESULTS A total of 3074 patients samples, 1465 (48%) were positive and 1609 (52%) were negative test results. The most common factors were rhinovirus 30.2%, influenza A 23.1%, and respiratory syncytial virus (RSV) A/B 19.1%, respectively. When the distribution of these three most common viruses by months is examined, the most frequent months were determined as June for rhinovirus, November for influenza A, and February for RSV A/B. In the period between October and February, there was a significant increase in the positivity level of viral factors. CONCLUSION The use of molecular methods in the diagnosis of respiratory infections will prevent unnecessary use of antibiotics and ensure correct and rapid treatment.

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Section: Discussionmentioning

confidence: 99%

Distribution of respiratory viruses: Evaluation of multiplex PCR results of 3074 patients

Zeytinli¹

2021

North Clin Istanbul

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Solunum Yolu Örnekleri̇nde İnsan Bocavi̇rus'ü Saptanmasi

TANRIVERDİ ÇAYCI,

ATEŞ,

GÜR VURAL

et al. 2023

Journal of Biotechnology and Strategic Health Research

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Objective: The aim of this study was to retrospectively examine the patients who presented with the complaints of respiratory tract infection and were found to have Human Bocavirus in the samples studied with the respiratory tract pathogens panel. Methods: We retrospectively analyzed patients of all age groups who were diagnosed with Human Bocavirus by PCR in the respiratory tract pathogens panel between January 2019 and November 2022. Results: Human Bocavirus was detected in 36 (3.64%) of 989 patients whose samples were studied with the respiratory tract pathogens panel. Fifteen of the patients (n:36) were under three years old. While Human Bocavirus was detected alone in 18 (50%) of the patients (n: 36), it was detected together with another factor in 18 (50%) patients. Conclusion: Human Bocavirus can be detected alone and/or as co-, infection in children with acute respiratory tract infection. The cause-effect relationship between Human Bocavirus and respiratory tract infection is not clearly known.

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Evaluation of the Performance of a Multiplex PCR Method for the Diagnosis of Viral Respiratory Tract Infections

Abstract: ÖZAmaç: Çalışmada, akut solunum yolu enfeksiyonu olan hastalarda solunum virüslerinin saptanmasında konvansiyonel yöntemlerle ["shell vial" hücre kültürü yöntemi, direkt

Cited by 2 publications

References 7 publications

Distribution of respiratory viruses: Evaluation of multiplex PCR results of 3074 patients

Distribution of respiratory viruses: Evaluation of multiplex PCR results of 3074 patients

Solunum Yolu Örnekleri̇nde İnsan Bocavi̇rus'ü Saptanmasi

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