Evaluation of the performance of protein separation in figure-8 centrifugal counter-current chromatography

Yang, Yi; Gu, Donghong; Aisa, Haji Akber; Ito, Yoichiro

doi:10.1016/j.jchromb.2011.10.019

Cited by 8 publications

(5 citation statements)

References 22 publications

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“…K values of hydrophilic compounds which were partitioned to the lower aqueous phase are very small in most of the common two-phase solvent systems [27]. Some polar two-phase solvent systems were used for the separation of water-soluble compounds such as carboxylic acids [28], sugars [29], nucleic acids [30], peptides [31][32][33][34][35] and proteins [36,37]. But these solvent systems are not suitable for phenolic compounds in the present study.…”

Section: Introductionmentioning

confidence: 91%

Bioassay-guided separation and purification of water-soluble antioxidants from Carthamus tinctorius L. by combination of chromatographic techniques

Yang

Hou

et al. 2013

Separation and Purification Technology

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Section: Introductionmentioning

confidence: 91%

Bioassay-guided separation and purification of water-soluble antioxidants from Carthamus tinctorius L. by combination of chromatographic techniques

Yang

Hou

et al. 2013

Separation and Purification Technology

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“…8). Compared with the Figure-8 column, the performance of centrifugal CCC was slightly improved by the ellipsoid column [18] . The above result suggests that higher efficiency of the protein separation can be obtained with a lower flow rate.…”

Section: Resultsmentioning

confidence: 99%

“…Recently, we found that the highest resolution (Rs) was obtained when the figure-8 column was mounted parallel to the radially acting centrifugal force field. The performance of the system was examined in various test samples including amino acids, dipeptides and proteins using moderately hydrophobic solvent system, high polarity solvent system and aqueous two-phase system [17, 18] . This high partition efficiency of this system may be explained by hydrodynamic motion and interaction of the two phases in the column at various angles.…”

Section: Introductionmentioning

confidence: 99%

Novel Design for Centrifugal Counter-Current Chromatography: VI. Ellipsoid Column

Yang

Xin

et al. 2014

Journal of Liquid Chromatography & Related Technologies

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A novel ellipsoid column was designed for centrifugal counter-current chromatography. Performance of the ellipsoid column with a capacity of 3.4 mL was examined with three different solvent systems composed of 1-butanol-acetic acid-water (4:1:5, v/v) (BAW), hexane-ethyl acetate-methanol-0.1 M HCl (1:1:1:1, v/v) (HEMH), and 12.5% (w/w) PEG1000 and 12.5% (w/w) dibasic potassium phosphate in water (PEG-DPP) each with suitable test samples. In dipeptide separation with BAW system, both stationary phase retention (Sf) and peak resolution (Rs) of the ellipsoid column were much higher at 0° column angle (column axis parallel to the centrifugal force) than at 90° column angle (column axis perpendicular to the centrifugal force), where elution with the lower phase at a low flow rate produced the best separation yielding Rs at 2.02 with 27.8% Sf at a flow rate of 0.07 ml/min. In the DNP-amino acid separation with HEMW system, the best results were obtained at a flow rate of 0.05 ml/min with 31.6% Sf yielding high Rs values at 2.16 between DNP-DL-glu and DNP-β-ala peaks and 1.81 between DNP-β-ala and DNP-L-ala peaks. In protein separation with PEG-DPP system, lysozyme and myolobin were resolved at Rs of 1.08 at a flow rate of 0.03 ml/min with 38.9% Sf. Most of those Rs values exceed those obtained from the figure-8 column under similar experimental conditions previously reported.

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“…The designs have been applied to the separation of two dipeptides with BuAaWat and three DNP-amino acids with HEMWat (0.01 M HCl). Protein separations have also been attempted with a figure-8 configuration disk. − …”

Section: Instrumentationmentioning

confidence: 99%

Countercurrent Separation of Natural Products: An Update

et al. 2015

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This work assesses the current instrumentation, method development, and applications in countercurrent chromatography (CCC) and centrifugal partition chromatography (CPC), collectively referred to as countercurrent separation (CCS). The article provides a critical review of the CCS literature from 2007 since our last review (J. Nat. Prod.2008, 71, 1489–1508), with a special emphasis on the applications of CCS in natural products research. The current state of CCS is reviewed in regard to three continuing topics (instrumentation, solvent system development, theory) and three new topics (optimization of parameters, workflow, bioactivity applications). The goals of this review are to deliver the necessary background with references for an up-to-date perspective of CCS, to point out its potential for the natural product scientist, and thereby to induce new applications in natural product chemistry, metabolome, and drug discovery research involving organisms from terrestrial and marine sources.

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Evaluation of the performance of protein separation in figure-8 centrifugal counter-current chromatography

Cited by 8 publications

References 22 publications

Bioassay-guided separation and purification of water-soluble antioxidants from Carthamus tinctorius L. by combination of chromatographic techniques

Bioassay-guided separation and purification of water-soluble antioxidants from Carthamus tinctorius L. by combination of chromatographic techniques

Novel Design for Centrifugal Counter-Current Chromatography: VI. Ellipsoid Column

Countercurrent Separation of Natural Products: An Update

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