Evaluation of the Poly(lactic‐co‐glycolic acid)/Pluronic F127 for Injection Laryngoplasty in Rabbits

Lee, Jin Ho; Kim, Dong Wook; Kim, Eun Na; Park, Seok Won; Kim, Hee Bok; Oh, Se Heang; Kwon, Seong Keun

doi:10.1177/0194599814549527

Cited by 11 publications

(12 citation statements)

References 22 publications

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“…High-speed camera examination revealed regular and symmetric collision of VF mucosa without distorted movement, confirming its suitability for injection and offering potential additional advantages of incorporating growth factors or delivering drugs in the vocal folds. [134]…”

Section: Biomaterials In Development/researchmentioning

confidence: 99%

Tissue engineering-based therapeutic strategies for vocal fold repair and regeneration

Stiadle

Lau

et al. 2016

Biomaterials

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Vocal folds are soft laryngeal connective tissues with distinct layered structures and complex multicomponent matrix compositions that endow phonatory and respiratory functions. This delicate tissue is easily damaged by various environmental factors and pathological conditions, altering vocal biomechanics and causing debilitating vocal disorders that detrimentally affect the daily lives of suffering individuals. Modern techniques and advanced knowledge of regenerative medicine have led to a deeper understanding of the microstructure, microphysiology, and micropathophysiology of vocal fold tissues. State-of-the-art materials ranging from extracecullar-matrix (ECM)-derived biomaterials to synthetic polymer scaffolds have been proposed for the prevention and treatment of voice disorders including vocal fold scarring and fibrosis. This review intends to provide a thorough overview of current achievements in the field of vocal fold tissue engineering, including the fabrication of injectable biomaterials to mimic in vitro cell microenvironments, novel designs of bioreactors that capture in vivo tissue biomechanics, and establishment of various animal models to characterize the in vivo biocompatibility of these materials. The combination of polymeric scaffolds, cell transplantation, biomechanical stimulation, and delivery of antifibrotic growth factors will lead to successful restoration of functional vocal folds and improved vocal recovery in animal models, facilitating the application of these materials and related methodologies in clinical practice.

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Section: Biomaterials In Development/researchmentioning

confidence: 99%

Tissue engineering-based therapeutic strategies for vocal fold repair and regeneration

Stiadle

Lau

et al. 2016

Biomaterials

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“…Their study did not note any material migration over time in the PCL group. The same group also utilized PLGA in a similar rabbit model of IL . PLGA carries a distinct advantage in that the biodegradation rate is controllable based on the polymer ratios.…”

Section: Discussionmentioning

confidence: 99%

“…The same group also utilized PLGA in a similar rabbit model of IL. 7 PLGA carries a distinct advantage in that the biodegradation rate is controllable based on the polymer ratios. They were able to demonstrate minimal inflammatory reaction after IL and some resorption of material by 8 weeks.…”

Section: Discussionmentioning

confidence: 99%

“…Given these shortcomings, additional work to optimize injection material is warranted. Recent efforts to move toward a more ideal injectable have focused on either biological enhancement of traditional materials or use of long‐lasting synthetic biopolymers to enhance injection longevity . We hypothesized that we could create long‐lasting biomaterial using tissue‐engineering principles by utilizing a bioscaffold, a cell source, and growth factors (GFs) to create a novel, self‐sustaining biomaterial .…”

Section: Introductionmentioning

confidence: 99%

“…Recent efforts to move toward a more ideal injectable have focused on either biological enhancement of traditional materials or use of long-lasting synthetic biopolymers to enhance injection longevity. [5][6][7][8] We hypothesized that we could create long-lasting biomaterial using tissueengineering principles by utilizing a bioscaffold, a cell source, and growth factors (GFs) to create a novel, selfsustaining biomaterial. 9 Our aim was to create a material with cells that had a chondrocytic phenotype to allow for long-lasting medialization of the vocal cord via a transoral approach.…”

Section: Introductionmentioning

confidence: 99%

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Preliminary results of tissue‐engineered injection laryngoplasty material in a rabbit model

et al. 2017

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Objectives/Hypothesis: Design and test a novel biomaterial for injection laryngoplasty aimed to increase the duration of effectiveness of micronized acellular dermis.Study Design: Animal model. Methods: Injection laryngoplasty was performed in three groups (n 5 5) of New Zealand White rabbits. Acellular dermis was either used alone as a control (group 1), was combined with undifferentiated stem cells (group 2), or with predifferentiated chondrocytic cells (group 3). Groups 2 and 3 were supplemented with growth factors. Animals were sacrificed 4 and 12 weeks after laryngoplasty and histologic analysis was completed. The major outcome measure was volume of tissue remaining.Results: After 4 weeks, the mean volume of tissue remaining was 341 6 89 mm 3 , 295 6 102 mm 3 , and 133 6 15 mm 3 , for groups 1 to 3, respectively. At the 12-week time point, volumes were 62 6 62 mm 3 , 235 6 35 mm 3 , and 107 6 99 mm 3 . After 12 weeks, there was a significantly higher volume in group 2 compared to group 1 or 3 (P 5 .01, P 5 .04). Volumes between week 4 and week 12 were significantly lower in group 1 (P 5 .02), but not significantly different for groups 2 and 3 (P 5 .38, P 5 .74). Histologic evaluation revealed a robust lymphocytic infiltration in all cases as well as morphologic and immunophenotypic features suggestive of chondrogenic differentiation in a single animal.Conclusions: Micronized acellular dermis combined with stem cells and growth factors showed significantly less resorption 12 weeks after injection laryngoplasty compared to micronized acellular dermis alone. Groups using novel tissue-engineered biomaterial showed a lower resorption rate over time compared with acellular dermis alone.

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Recombinant Resilin‐Based Bioelastomers for Regenerative Medicine Applications

Mahara

Tong

et al. 2015

Adv Healthcare Materials

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The superior elasticity, excellent resilience at high-frequency, and hydrophilic capacity of natural resilin have motivated investigations of recombinant resilin-based biomaterials as a new class of bio-elastomers in the engineering of mechanically active tissues. Accordingly, we report here the comprehensive characterization of modular resilin-like polypeptide (RLP) hydrogels and introduce their suitability as a novel biomaterial for in vivo applications. Oscillatory rheology confirmed that a full suite of the RLPs can be rapidly crosslinked upon addition of the tris(hydroxymethyl phosphine) (THP) cross-linker, achieving similar in situ shear storage moduli (20k±3.5Pa) across various material compositions. Uniaxial stress relaxation tensile testing of hydrated RLP hydrogels under cyclic loading and unloading showed negligible stress reduction and hysteresis, superior reversible extensibility, and high resilience with Young’s moduli of 30±7.4kPa. RLP hydrogels containing MMP-sensitive domains are susceptible to enzymatic degradation by MMP-1. Cell culture studies revealed that RLP-based hydrogels supported the attachment and spreading (2D) of human mesenchymal stem cells (hMSCs) and did not activate cultured macrophages. Subcutaneous transplantation of RLP hydrogels in a rat model, which to our knowledge is the first such reported in vivo analysis of RLP-based hydrogels, illustrated that these materials do not elicit a significant inflammatory response, suggesting their potential as materials for tissue engineering applications with targets of mechanically demanding tissues such as vocal fold and cardiovascular tissues.

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Evaluation of the Poly(lactic‐co‐glycolic acid)/Pluronic F127 for Injection Laryngoplasty in Rabbits

Abstract: As a vocal fold injection material, PLGA/Pluronic F127 showed a good bio-compatibility without significant inflammatory response. Further experiment will follow to elucidate its role for drug or gene delivery into the vocal fold.

Cited by 11 publications

References 22 publications

Tissue engineering-based therapeutic strategies for vocal fold repair and regeneration

Tissue engineering-based therapeutic strategies for vocal fold repair and regeneration

Preliminary results of tissue‐engineered injection laryngoplasty material in a rabbit model

Recombinant Resilin‐Based Bioelastomers for Regenerative Medicine Applications

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