Evaluation of the Potency of the Pertussis Vaccine in Experimental Infection Model with Bordetella pertussis: Study of the Case of the Pertussis Vaccine Used in the Expanded Vaccination Program in Algeria

Abstract

In Algeria, vaccination against pertussis is carried out using the whole-cell pertussis vaccine combined with the diphtheria and tetanus toxoids (DTwp). The quality control of vaccines locally produced or imported is carried out before the batch release. The aim of our work was to evaluate the potency of pertussis vaccines. In the present study, five consecutive trials of potency were conducted on samples of the same batch of (DTwp) using the mouse protection test (MPT) against experimental infection of Bordetella pertussis strain 18323, based on the Kendrick test. The virulence of B. pertussis strain 18–323 was verified by the mortality of mice, with an average LD50 of 338.92, as well as the dose of the lethal test containing a mean number of LD50 of 324.43. The (MPT) test recorded a relative potency of 8.02 IU/human dose, with 95% CL of (3.56–18.05) IU/human dose. The development of the (MPT) at the laboratory of quality control of vaccines and sera at the Pasteur Institute of Algeria was effective in evaluating the potency of whole-cell pertussis vaccines. Interestingly, our study indicates that this potency is necessary for the vaccine quality assurance. Further validation is needed to strengthen the application and routine use of the test.

Evaluating Agile Neural Educational System for Effective Resource Management

“…This should be strictly monitored. As indicated by Tahar Djebbar et al (2022), system evaluation is essential for enhancing efficiency. The system model in Figure 3 is the proposed solution to the problem identified.…”

“…Understanding a complete system and evaluating it accurately is essential for enhancing the efficiency of that system (Tahar Djebbar et al, 2022). Interrelated components of a system must work together and be evaluated as a whole system.…”

“…In a climate evaluation model, (Eyring et al, 2019) comprehensive and rapid evaluation of the model to enhance efficiency in climate assessment proves more successful through the Coupled Model Intercomparison Project (CMIP), which helps in sharing, comparing and analyzing global climate issues. In a paper by Tahar Djebbar et al (2022), the potency of a vaccine is evaluated through an experimental model.…”

“…In some counties, the learner progresses until age 17 for the combined junior High and Senior High School. Figure 2 shows the universal structure of basic education System Evaluation Understanding a complete system and evaluating it accurately is essential for enhancing the efficiency of that system (Tahar Djebbar et al, 2022). Interrelated components of a system must work together and be evaluated as a whole system.…”

Evaluating Agile Neural Educational System for Effective Resource Management.

“…This should be strictly monitored. As indicated by (Tahar djebbar et al, 2022), system evaluation is essential for enhancing efficiency. The system model in Fig 3 is the proposed solution to the problem identified.…”