Evaluation of the Potential In Vitro Antiproliferative Effects of Millimeter Waves at Some Therapeutic Frequencies on RPMI 7932 Human Skin Malignant Melanoma Cells

Beneduci, Amerigo

doi:10.1007/s12013-009-9053-8

Cited by 27 publications

(20 citation statements)

References 28 publications

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“…However, these results have not been reproduced, and other studies have showed that neither the cell doubling time nor the cell cycle is significantly modified by MMW exposure [Beneduci, 2009;Zhadobov et al, 2009]. At the molecular level, 60 GHz radiation below 1 mW/cm 2 can induce structural modifications in artificial biomembranes [Zhadobov et al, 2006].…”

Section: Mmw At Intermediate Pd (5-15 Mw/cmmentioning

confidence: 89%

Whole‐genome expression analysis in primary human keratinocyte cell cultures exposed to 60 GHz radiation

Quément

Nicolaz

Desmots

et al. 2011

Bioelectromagnetics

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The main purpose of this study is to investigate potential responses of skin cells to millimeter wave (MMW) radiation increasingly used in the wireless technologies. Primary human skin cells were exposed for 1, 6, or 24 h to 60.4 GHz with an average incident power density of 1.8 mW/cm(2) and an average specific absorption rate of 42.4 W/kg. A large-scale analysis was performed to determine whether these exposures could affect the gene expression. Gene expression microarrays containing over 41,000 unique human transcript probe sets were used, and data obtained for sham and exposed cells were compared. No significant difference in gene expression was observed when gene expression values were subjected to a stringent statistical analysis such as the Benjamini-Hochberg procedure. However, when a t-test was employed to analyze microarray data, 130 transcripts were found to be potentially modulated after exposure. To further quantitatively analyze these preselected transcripts, real-time PCR was performed on 24 genes with the best combination of high fold change and low P-value. Five of them, namely CRIP2, PLXND1, PTX3, SERPINF1, and TRPV2, were confirmed as differentially expressed after 6 h of exposure. To the best of our knowledge, this is the first large-scale study reporting on potential gene expression modification associated with MMW radiation used in wireless communication applications.

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Section: Mmw At Intermediate Pd (5-15 Mw/cmmentioning

confidence: 89%

Whole‐genome expression analysis in primary human keratinocyte cell cultures exposed to 60 GHz radiation

Quément

Nicolaz

Desmots

et al. 2011

Bioelectromagnetics

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“…As noted before, studies aimed at the evaluation of the potential biological effects of mmwaves [12,[52][53][54][55], are largely performed on in vitro cell cultures exposed under far-field radiation conditions from the bottom of the culture plate [36,56,57]. It is interesting therefore to estimate the deviation of the open-ended waveguide reflectivity data from those corresponding to far-field exposure.…”

Section: Petri Dish Reflectivity Under Far-field Exposurementioning

confidence: 99%

Open-Ended Waveguide Measurement and Numerical Simulation of the Reflectivity of Petri Dish Supported Skin Cell Monolayers in the mm-wave Range

Beneduci

Chidichimo

2012

J Infrared Milli Terahz Waves

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Open-ended waveguide reflectometry is a promising tool for permittivity and other material properties calculation at mm-waves (30-300 GHz). Measurement of the reflection coefficient does not require sample manipulation, allowing in vivo and in vitro non destructive studies on cells. Here we used this technique for measuring the power reflection coefficient (reflectivity) of water and Petri dish supported human skin melanoma and keratinocyte cell cultures, in the 53-72 GHz frequency range. The dependence of the reflectivity on polystyrene or glass thickness of the Petri base plate and on the cell layer thickness was analyzed. Permittivity data were then easily retrieved by using a plane wave-dominant mode approach for formulating the reflectivity at the aperture of the flange-mounted open-ended rectangular waveguide probe. Limits and validity of such an approximate approach were analyzed and compared with full-wave near field formulations for which magnitude and phase of the reflection coefficient must be measured and solved using complicated systems of integral equations and extensive numerical calculation. Finally, Petri dish reflectivity measured by the open-ended waveguide method was compared with that numerically simulated under far-field exposure conditions used in a large number of in vitro studies. Such an analysis showed that, under certain conditions, open-ended reflectivity values approach the far field ones.

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“…The power density of this therapy used in medicine varies from 1 to 10 mw/cm 2 , and is applied in experimental and clinical oncology (3,4). While it is well established that at high power densities, millimeter wave radiation induces thermal increase on the exposed biosystems whose mechanisms could be non-thermal and of resonant nature or at least frequency-specific (5). Previously studies demonstrated millimeter wave radiation in treatment for various types of malignant tumors.…”

Section: Introductionmentioning

confidence: 99%

Millimeter wave radiation induces apoptosis via affecting the ratio of Bax/Bcl-2 in SW1353 human chondrosarcoma cells

Ye²,

Cai³

et al. 2011

Oncol Rep

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Abstract. The efficacy and safety of millimeter wave radiation has been proven for various types of malignant tumors. However, the mechanisms underlying effects of millimeter wave radiation on apoptosis are still unclear. The present study was undertaken to examine the effects of millimeter wave radiation on cell apoptosis and mitochondrial membrane potential, and to determine the molecular mechanism of millimeter wave radiation-induced apoptosis by investigating the expression of Bcl-2 family proteins (Bcl-2, Bax), caspase-9 and caspase-3 in SW1353 cells. We found that millimeter wave radiation suppressed the viability of SW1353 cells, demonstrating that millimeter wave radiation induced cell apoptosis and reduced cell viability in a time-dependent manner. Furthermore, we observed that treatment of cells with millimeter wave radiation significantly induced loss of mitochondrial membrane potential, upregulated proapoptotic Bax, caspase-9 and caspase-3, but did not significantly change levels of antiapoptotic Bcl-2. These data suggested that millimeter wave radiation may induce apoptosis via affecting the ratio of Bax/Bcl-2 in SW1353 cells.

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Evaluation of the Potential In Vitro Antiproliferative Effects of Millimeter Waves at Some Therapeutic Frequencies on RPMI 7932 Human Skin Malignant Melanoma Cells

Cited by 27 publications

References 28 publications

Whole‐genome expression analysis in primary human keratinocyte cell cultures exposed to 60 GHz radiation

Whole‐genome expression analysis in primary human keratinocyte cell cultures exposed to 60 GHz radiation

Open-Ended Waveguide Measurement and Numerical Simulation of the Reflectivity of Petri Dish Supported Skin Cell Monolayers in the mm-wave Range

Millimeter wave radiation induces apoptosis via affecting the ratio of Bax/Bcl-2 in SW1353 human chondrosarcoma cells

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