Evaluation of the potential of kartogenin encapsulated poly(L-lactic acid-co-caprolactone)/collagen nanofibers for tracheal cartilage regeneration

Abstract

Tracheal stenosis is one of major challenging issues in clinical medicine because of the poor intrinsic ability of tracheal cartilage for repair. Tissue engineering provides an alternative method for the treatment of tracheal defects by generating replacement tracheal structures. In this study, we fabricated coaxial electrospun fibers using poly(L-lactic acid-co-caprolactone) and collagen solution as shell fluid and kartogenin solution as core fluid. Scanning electron microscope and transmission electron microscope images demonstrated that nanofibers had uniform and smooth structure. The kartogenin released from the scaffolds in a sustained and stable manner for about 2 months. The bioactivity of released kartogenin was evaluated by its effect on maintain the synthesis of type II collagen and glycosaminoglycans by chondrocytes. The proliferation and morphology analyses of mesenchymal stems cells derived from bone marrow of rabbits indicated the good biocompatibility of the fabricated nanofibrous scaffold. Meanwhile, the chondrogenic differentiation of bone marrow mesenchymal stem cells cultured on core-shell nanofibrous scaffold was evaluated by real-time polymerase chain reaction. The results suggested that the core-shell nanofibrous scaffold with kartogenin could promote the chondrogenic differentiation ability of bone marrow mesenchymal stem cells. Overall, the core-shell nanofibrous scaffold could be an effective delivery system for kartogenin and served as a promising tissue engineered scaffold for tracheal cartilage regeneration.

Combining Stem Cells and Biomaterial Scaffolds for Constructing Tissues and Cell Delivery

“…Electrospinning poses challenges in terms of maintaining the bioactivity of the protein, but it can be achieved under appropriate conditions [52,53]. Collagen is often added to synthetic polymer scaffolds to enhance their bioactivity and enable stem cells to bind to these scaffolds [54,55]. Both collagen and gelatin microspheres can be seeded with stem cells as a novel approach to tissue engineering.…”

Transplantation of a 3D-printed tracheal graft combined with iPS cell-derived MSCs and chondrocytes

“…Successful tracheal reconstruction using tissue-engineered artificial trachea requires biocompatible 3D constructs comparable with natural trachea, coverage with ciliated respiratory mucosa, and adequate cartilage remodeling [21][22][23][24] . Many studies have documented the induction of tracheal mucosa and cartilage using a variety of scaffolds.…”

Tissue engineering applications in otolaryngology—The state of translation

“…Heatmap representing the proportion of publications by year that utilized specific translational research methodologies from construct characterization to human trial to investigate laryngeal tissue engineering 85‐87,91,92,94,96,97,198‐306 …”