Evaluation of the potential role of diethylstilbestrol on the induction of endometriosis in a rat model – An alternative approach

Krishnamoorthy, Sathiya-Priya; Kalimuthu, Vignesh; Manimegalai, Swathi Chandran; Arulanandu, Angel; Ramesh, Thiyagarajan; Kadalmani, Balamuthu

doi:10.1016/j.bbrc.2022.05.092

Cited by 7 publications

(7 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The estrus cylicity was continuously monitored in both the control and endo group animals to confirm the regularity and alterations in the estrus cycle and the body weight was also monitored. The endo group displayed alterations in the estrus cycle showing irregular pattern of the cycle and the body weight which is evidenced in the previous study 13 . Further to confirm the induction, the experimental animals were analyzed for the levels of CA-125 which is an effective biomarker in the diagnosis of endometriosis.…”

Section: Higher Levels Of Ca-125 Confirms the Endometriosis Inductionsupporting

confidence: 75%

“…Endometriosis was induced in the rat model which was described in previous study. 13 Briefly, the animals showing regular estrus cycles were chosen for the study and grouped into Control (N=3) and Endo (N=3). Endo group animals received DES (Diethylstilbestrol, Sigma Aldrich, USA) for 20 days at a concentration of 1mg/kg/bw via subcutaneous route of injection.…”

Section: Endometriosis Inductionmentioning

confidence: 99%

“…12 Our previous study demonstrated the successful development of endometriosis by diethylstilbestrol (DES) instead of the traditional surgical induction in the animal model. 13 Further in this study we aimed to prove endometriosis induction of diethylstilbestrol in animal model ensues via immuneinflammatory pathway.…”

Section: Introductionmentioning

confidence: 97%

See 2 more Smart Citations

Evidence Supporting the Diethylstilbestrol Induction of Endometriosis Via Immune-Inflammatory Pathway in Rat Model

Krishnamoorthy

Kadalmani

2022

Int J Life Sci Pharm Res

Self Cite

View full text Add to dashboard Cite

Endometriosis is a benign reproductive disorder distinguished by chronic inflammation and anomalous progress of stromal cells and glands outside the uterus. Though the etiology of endometriosis is still a matter of debate, the immuneinflammatory mediators are thought to play a major role. Macrophage infiltration and proinflammatory intermediaries in the peritoneal environment peritoneal milieu impact ovarian, uterine function and pelvic morphology, resulting in endometriosis symptoms and signs. The endometriosis model system was developed using diethylstilbestrol (DES) which is examined to develop the disease via the immune-inflammatory pathway. The immune-inflammatory markers (NFKB, MAPK, TNF-α, IL-6 and STAT- 3) were investigated in this study. The results demonstrated that the DES induced endometriosis animal model had a higher level of CA-125, a biomarker of endometriosis. Endometriosis-induced groups had increased levels of NFKB, MAPK, TNF-α, IL-6 and STAT-3 which clearly depicts the immune-inflammatory role in the induction. Finally, the results conclude that DES induced endometriosis via the immune-inflammatory pathway.

show abstract

Section: Higher Levels Of Ca-125 Confirms the Endometriosis Inductionsupporting

confidence: 75%

Section: Endometriosis Inductionmentioning

confidence: 99%

See 1 more Smart Citation

Evidence Supporting the Diethylstilbestrol Induction of Endometriosis Via Immune-Inflammatory Pathway in Rat Model

Krishnamoorthy

Kadalmani

2022

Int J Life Sci Pharm Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…The risk of developing EMs in adult life was significantly increased by exposure to DES in utero (76). A case report noted that all daughters and subsequent granddaughters who were prenatally exposed to DES presented with EMs, suggesting a role of DES in the pathogenesis of this disease (77). Animal studies have shown that DES induces erratic estrous cycles and abnormal hormone levels, demonstrating its efficacy in inducing EMs and suggesting its potential as an alternative approach in rat models (78).…”

Section: Other Edcsmentioning

confidence: 99%

“…A case report noted that all daughters and subsequent granddaughters who were prenatally exposed to DES presented with EMs, suggesting a role of DES in the pathogenesis of this disease ( 77 ). Animal studies have shown that DES induces erratic estrous cycles and abnormal hormone levels, demonstrating its efficacy in inducing EMs and suggesting its potential as an alternative approach in rat models ( 78 ). Integrating metabolic and cytokine profiling, a comprehensive approach has provided support for the association between EMs and certain persistent organic pollutants (POPs) like trans-nonachlor, polychlorinated biphenyls (PCBs) 114 and dioxin-like toxic equivalents from PCBs ( 79 ).…”

Section: Introductionmentioning

confidence: 99%

The adverse role of endocrine disrupting chemicals in the reproductive system

Pan,

Liu,

et al. 2024

Front. Endocrinol.

View full text Add to dashboard Cite

Reproductive system diseases pose prominent threats to human physical and mental well-being. Besides being influenced by genetic material regulation and changes in lifestyle, the occurrence of these diseases is closely connected to exposure to harmful substances in the environment. Endocrine disrupting chemicals (EDCs), characterized by hormone-like effects, have a wide range of influences on the reproductive system. EDCs are ubiquitous in the natural environment and are present in a wide range of industrial and everyday products. Currently, thousands of chemicals have been reported to exhibit endocrine effects, and this number is likely to increase as the testing for potential EDCs has not been consistently required, and obtaining data has been limited, partly due to the long latency of many diseases. The ability to avoid exposure to EDCs, especially those of artificially synthesized origin, is increasingly challenging. While EDCs can be divided into persistent and non-persistent depending on their degree of degradation, due to the recent uptick in research studies in this area, we have chosen to focus on the research pertaining to the detrimental effects on reproductive health of exposure to several EDCs that are widely encountered in daily life over the past six years, specifically bisphenol A (BPA), phthalates (PAEs), polychlorinated biphenyls (PCBs), parabens, pesticides, heavy metals, and so on. By focusing on the impact of EDCs on the hypothalamic-pituitary-gonadal (HPG) axis, which leads to the occurrence and development of reproductive system diseases, this review aims to provide new insights into the molecular mechanisms of EDCs’ damage to human health and to encourage further in-depth research to clarify the potentially harmful effects of EDC exposure through various other mechanisms. Ultimately, it offers a scientific basis to enhance EDCs risk management, an endeavor of significant scientific and societal importance for safeguarding reproductive health.

show abstract

Association of Atrazine-Induced Overexpression of Aldo–Keto-Reductase 1C2 (AKR1C2) with Hypoandrogenism and Infertility: An Experimental Study in Male Wistar Rat

Arulanandu,

Kalimuthu,

Manimegalai

et al. 2024

Reprod. Sci.

View full text Add to dashboard Cite

Evaluation of the potential role of diethylstilbestrol on the induction of endometriosis in a rat model – An alternative approach

Cited by 7 publications

References 32 publications

Evidence Supporting the Diethylstilbestrol Induction of Endometriosis Via Immune-Inflammatory Pathway in Rat Model

Evidence Supporting the Diethylstilbestrol Induction of Endometriosis Via Immune-Inflammatory Pathway in Rat Model

The adverse role of endocrine disrupting chemicals in the reproductive system

Association of Atrazine-Induced Overexpression of Aldo–Keto-Reductase 1C2 (AKR1C2) with Hypoandrogenism and Infertility: An Experimental Study in Male Wistar Rat

Contact Info

Product

Resources

About