Evaluation of the precision-cut liver and lung slice systems for the study of induction of CYP1, epoxide hydrolase and glutathione S-transferase activities

Modulation of rat pulmonary carcinogen-metabolising enzyme systems by the isothiocyanates erucin and sulforaphane

“…It is particularly suited to the lung, a heterogeneous tissue composed of many different cell types that differ markedly in their carcinogen-metabolising enzyme profile [17]. Studies emanating from our laboratory have established that precision-cut slices are an appropriate system for use in evaluating the potential of xenobiotics to modulate cytochrome P450 and phase II activities in the lung [18].…”

Differential response of human and rat epoxide hydrolase to polycyclic aromatic hydrocarbon exposure: Studies using precision-cut tissue slices

“…By contrast, studies performed using primary human primary hepatocyte cultures demonstrated that a number of xenobiotics could elicit a modest increase in epoxide hydrolase mRNA levels, although this was not translated to the protein level; PAHs were not investigated in this study [11]. In more recent studies emanating from our laboratory employing precision-cut rat liver and lung slices, it was demonstrated that the prototypical PAH benzo[a]pyrene was a modest inducer of epoxide hydrolase activity, and protein and mRNA expression [12].…”

“…The optimal conditions of exposure, e.g. concentration range and incubation time, for both liver and lung slices have already been established [12], and were adopted in the current studies.…”

“…For the quantitative reverse transcription-polymerase chain reaction, the primer and TAMRA/FAM dual labelled probe [12] were designed using the Primer Express software (Applied Biosystems, California, USA) and purchased from MWG, Ebersberg, Germany. Each primer and probe set was designed to amplify sequences within a single exon, so that genomic DNA could be used as a standard.…”

Primary Liver Cells Cultured on Carbon Nanotube Substrates for Liver Tissue Engineering and Drug Discovery Applications

“…Liver toxicity (hepatotoxicity) is responsible for a high percentage of late-stage attrition in drug development and on-market withdrawals. [1][2] A number of liver models have been proposed, ranging from computational [3][4] , through liver slices 5 , to cultures of primary or secondary liver cells. 6 Cultured primary hepatocytes are currently seen as the gold-standard for drug testing, but it is acknowledged that they rapidly alter their phenotype in culture, meaning that screening assays must be carefully validated, and complicating extrapolation to in vivo.…”