Evaluation of the premature chromosome condensation scoring protocol after proton and X-ray irradiation of human peripheral blood lymphocytes at high doses range

Rawojć, K.; Miszczyk, Justyna; Możdżeń, A.; Swakoń, Jan; Sowa‐Staszczak, Anna

doi:10.1080/09553002.2018.1490038

Cited by 8 publications

(21 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Besides, the CD and DAP values of patients from group D rose markedly in contrast to the values of group A, B, and C (P < 0.05). is was in line with the research results of Rawojć et al [16]. rough the above results, there were obvious differences in the radiation dose of patients from each group.…”

Section: Discussionsupporting

confidence: 92%

Image Mosaic Algorithm-Based Analysis of Effects of Low-Dose X-Rays on Blood Immune Cells

Miao

Guo

et al. 2021

Scientific Programming

View full text Add to dashboard Cite

This work aimed to explore the effects of image mosaic algorithm- (IMA-) based low-dose X-rays on deoxyribonucleic acid (DNA) damage of peripheral blood lymphocytes in cardiovascular patients. 84 patients with interventional cardiovascular surgeries were selected as the research objects and divided into coronary intervention surgery (A) group, coronary angiography (B) group, radiofrequency ablation (C) group, and permanent cardiac pacemaker implantation (D) group. They were treated with IMA-based low-dose X-rays, DNA damage indicators of lymphocytes were detected, and the relationship was analyzed between those indicators and radiation dose. Results showed that the cumulative dose (CD) and dose area product (DAP) of group A were the lowest, and those of group D were obviously higher than those of the other three groups ( P < 0.01 ). Besides, the results also showed that the values of CD and DAP of patients from group A and group D were 0.38 ± 0.08 (Gy) and 0.38 ± 0.08 (Gy/cm2) and 1.37 ± 0.21 (Gy) and 1.37 ± 0.21(Gy/cm2), respectively. The comet tail DNA content (TDNA) and tail moment (TM) of each group after surgery increased dramatically compared with before surgery ( P < 0.01 ), and the TDNA and TM of group D were greatly higher than those of the other three groups ( P < 0.01 ). Therefore, TDNA and TM were markedly positively correlated with DAP and CD ( P < 0.01 ). IMA-based X-ray radiotherapy caused reliable DNA damage to peripheral blood lymphocytes of cardiovascular patients, and the greater the radiation dose, the more serious the DNA damage.

show abstract

Section: Discussionsupporting

confidence: 92%

Image Mosaic Algorithm-Based Analysis of Effects of Low-Dose X-Rays on Blood Immune Cells

Miao

Guo

et al. 2021

Scientific Programming

View full text Add to dashboard Cite

show abstract

“…The frequency of micronuclei (MNi) and excess PCC fragments measured with the CBMN and PCC techniques are internationally accepted biomarkers of individual dose assessment in the field of biological dosimetry and radiation protection [7][8][9][10][11]. They are used to evaluate in vivo radiation exposure of occupational, medical and accidentally exposed individuals, usually in their HPBLs [7][8][9][10][11]. The advantage of these tests consists of providing simultaneous information on chromosomal damage and the rate of lymphocyte proliferation [7,8].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the nuclear medicine personnel occupational exposure to radioiodine

Miszczyk

Rawojć

Panek

et al. 2019

European Journal of Radiology

View full text Add to dashboard Cite

DENM) who are occupationally exposed to 131 I. Materials and Methods: The exposure was monitored by whole-body and finger ring dosimeters. The thyroid iodine intake was measured by a whole-body spectrometer equipped with two semiconductor gamma radiation detectors. A cytokinesis-block micronucleus assay and the premature chromosome condensation technique were used to assess the aberration score. Cytogenetic analyses were carried out on a group of 29 workers and were compared to 32 controls (healthy donors), matched for gender and age. Results: On average, the exposed group showed a significantly higher frequency of genetic damage and a higher proliferation index compared to the control group. Smoking status, age and duration of exposure influenced the observed effects in both groups. No differences in measured biomarkers were observed after stratification of the exposed group into two subgroups based on the measured 131 I activity below and above 6 Bq. Conclusion:The findings suggest that radiation protection principles based on whole-body and finger ring dosimetry, supported by activity measurements with a whole-body spectrometer, may be insufficient to monitor the absorbed dose estimation of the nuclear medicine staff who are occupationally exposed to 131 I. Furthermore, their future health risks are influenced by confounders. Direct assessments comparing physical and biological dose estimations on the larger group are needed to accurately monitor occupational radiation exposure.

show abstract

“…Cultures were set up according to standard procedures [11,12]. Briefly, whole blood (0.5 ml) was added per 4.5 ml of the RPMI 1640 culture medium (PAA Laboratories GmbH Pasching, Austria) that was supplemented with 20% fetal calf serum (Gibco, Carlsbad, United States), antibiotics, and a small amount of bromodeoxyuridine.…”

Section: Culturing Slide Preparationmentioning

confidence: 99%

“…In the case of PCC culture, in exact 30 min before ending the culturing process calyculin A (50 nM) was added to the culture medium. Then, the cells were fixed as well as stained with Giemsa for PCC and a fluorescence plus Giemsa solution ((Merck Millipore, Darmstadt, Germany) for chromosome preparations following a standard procedure described elsewhere [11]. A conventional dose-effect curve obtained from in vitro irradiation experiments using X-rays (250 kV, 10 mA) was used to estimate the exposure dose of victims.…”

Section: Culturing Slide Preparationmentioning

confidence: 99%

See 1 more Smart Citation

Application of Premature Chromosome Condensation and Dicentric Analysis in Retrospective Biological Dosimetry of Radiation Accident

et al. 2020

Self Cite

View full text Add to dashboard Cite

Cytogenetic dosimetry based on dicentric analysis and premature chromosome condensation plays an important role in the triage and medical management of affected people in radiological accidents. In our studies, blood samples from persons that were suspected of accidental exposure to ionizing radiation during interventional radiology procedure (plastic surgery of the renal arteries) were investigated for the purpose of the retrospective biological dosimetry of absorbed dose. In this case, the obligatory reported dose value, kerma area product, was high: 106.04 Gy cm 2 . The peak skin dose was reported by X-ray machine system as 17 Gy. Peak skin dose for an iso-centric interventional fluoroscope is measured at defined reference point, located 15 cm from the isocenter toward the X-ray tube. Due to the lack of possibility to connect kerma area product with an effect (kerma area product could be connected with the maximum local dose in the X-ray field) the patient's blood and 2 most-at-risk people (medical doctors) were investigated. In addition, 5 dosimeters for the whole body (Hp(10)) and 1 ring dosimeter measuring the skin exposure (Hp(0.07)) of medical workers participating in the radiological procedure were investigated. The measurements of individual doses by the thermoluminescent dosimeters were carried out by the Laboratory of Individual and Environmental Dosimetry (Polish acronym LADIS). To estimate the potentially absorbed dose lymphocytes from blood were analyzed for the presence of chromosomal aberrations (dicentrics and rings), which are the "gold standard" assay for biodosimetry. Additionally, the absorbed dose was monitored by the analysis of excess fragments with premature chromosome condensation technique, which is recommended for high doses and partial body exposure. Finally, estimated doses for obtained blood samples were compared to the dose-response curves for dicentrics and premature chromosome condensation excess fragments, detected in our laboratory after induction in human lymphocytes by in vitro X-ray irradiation.

show abstract

Evaluation of the premature chromosome condensation scoring protocol after proton and X-ray irradiation of human peripheral blood lymphocytes at high doses range

Cited by 8 publications

References 30 publications

Image Mosaic Algorithm-Based Analysis of Effects of Low-Dose X-Rays on Blood Immune Cells

Image Mosaic Algorithm-Based Analysis of Effects of Low-Dose X-Rays on Blood Immune Cells

Assessment of the nuclear medicine personnel occupational exposure to radioiodine

Application of Premature Chromosome Condensation and Dicentric Analysis in Retrospective Biological Dosimetry of Radiation Accident

Contact Info

Product

Resources

About