Evaluation of the presence of the bap gene in Staphylococcus aureus isolates recovered from human and animals species

Vautor, Eric; Abadie, G.; Pont, Allan; Thiery, Régis

doi:10.1016/j.vetmic.2007.08.018

Cited by 67 publications

(61 citation statements)

References 13 publications

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“…Our results emphasize the importance of this ica-independent biofilm formation pathway in the MRSA clinical strains tested, since only strong biofilm producer MRSA strains were positive for the fnbB gene. Concerning Bap, our findings seem to be consistent with other researchers, which have not reported this gene in any S. aureus isolate of human origin (Cucarella et al, 2001(Cucarella et al, , 2004Vautor et al, 2008). The clfA and sasC genes were widely distributed among strains without association with biofilm production, while bbp was absent in all tested strains.…”

Section: Discussionsupporting

confidence: 92%

Biofilm formation of Brazilian meticillin-resistant Staphylococcus aureus strains: prevalence of biofilm determinants and clonal profiles

Batistão

Campos

Camilo

et al. 2016

Journal of Medical Microbiology

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Biofilms plays an important role in medical-device-related infections. This study aimed to determine the factors that influence adherence and biofilm production, as well as the relationship between strong biofilm production and genetic determinants in clinical isolates of meticillin-resistant Staphylococcus aureus (MRSA). Fifteen strains carrying different chromosomal cassettes recovered from hospitalized patients were selected; five SCCmecII, five SCCmecIII and five SCCmecIV. The SCCmec type, agr group and the presence of the virulence genes (bbp, clfA, icaA, icaD, fnbB, bap, sasC and IS256) were assessed by PCR. PFGE and multilocus sequence typing (MLST) techniques were also performed. The initial adhesion and biofilm formation were examined by quantitative assays. The surface tension and hydrophobicity of the strains were measured by the contact angle technique to evaluate the association between these parameters and adhesion ability. SCCmecIII and IV strains were less hydrophilic, with a high value for the electron acceptor parameter and higher adhesion in comparison with SCCmecII strains. Only SCCmecIII strains could be characterized as strong biofilm producers. The PFGE showed five major pulsotypes (A-E); however, biofilm production was related to the dissemination of one specific PFGE clone (C) belonging to MLST ST239 (Brazilian epidemic clonal complex). The genes agrI, fnbB and IS256 in SCCmecIII strains were considered as genetic determinants associated with strong biofilm-formation by an icaindependent biofilm pathway. This study contributes to the understanding of biofilm production as an aggravating factor potentially involved in the persistence and severity of infections caused by multidrug-resistant MRSA belonging to this genotype.

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Section: Discussionsupporting

confidence: 92%

Biofilm formation of Brazilian meticillin-resistant Staphylococcus aureus strains: prevalence of biofilm determinants and clonal profiles

Batistão

Campos

Camilo

et al. 2016

Journal of Medical Microbiology

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“…Our positive control was staphylococcal strain 219R, isolated from a cow, which generated a band of the correct size with these primers, and when sequenced showed 98 % homology to the Staphylococcus xylosus bap gene. We note the low incidence of the bap gene in another animal strain collection (Vautor et al, 2008).…”

Section: Distribution Of Mobile Genetic Elements (Mges)mentioning

confidence: 79%

Staphylococcus aureus host specificity: comparative genomics of human versus animal isolates by multi-strain microarray

2008

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Staphylococcus aureus is a commensal and pathogen of several mammalian species, particularly humans and cattle. We aimed to (i) identify S. aureus genes associated with host specificity, (ii) determine the relatedness of human and animal isolates, and (iii) identify whether human and animal isolates typically exchanged mobile genetic elements encoding virulence and resistance genes. Using a well-validated seven-strain S. aureus microarray, we compared 56 UK S. aureus isolates that caused infection in cows, horses, goats, sheep and a camel with 161 human S. aureus isolates from healthy carriers and community acquired infections in the UK. We had previously shown that human isolates are clustered into ten dominant and a few minor lineages, each with unique combinations of surface proteins predicted to bind to human proteins. We found that the animal-associated S. aureus clustered into ten lineages, with 61 % assigned to four lineages, ST151, ST771, ST130 and ST873, that were unique to animals. The majority of bovine mastitis was caused by isolates of lineage ST151, ST771 and ST97, but a few human lineages also caused mastitis. S. aureus isolated from horses were more likely to cluster into humanassociated lineages, with 54 % of horse-associated S. aureus assigned to the human clusters CC1, CC8 and CC22; along with the presence of some multi-drug resistant strains, this suggests a human origin. This is the most comprehensive genetic comparison of human versus animal S. aureus isolates conducted, and because we used a whole-genome approach we could estimate the key genes with the greatest variability that are associated with host specificity. Several genes conserved in all human isolates were variable or missing in one or more animal lineages, including the well-characterized lineage specific genes fnbA, fnbB and coa. Interestingly, genes carried on mobile genetic elements (MGEs) such as chp, scn and sak were less common in animal S. aureus isolates, and bap was not found. There was a lot of MGE variation within lineages, and some evidence that exchange of MGEs such as bacteriophage and pathogenicity islands between animal and human lineages is feasible, but there was less evidence of antibiotic resistance gene transfer on the staphylococcal cassette chromosomes (SCC) or plasmids. Surprisingly, animal lineages are closely related to human lineages and only a handful of genes or gene combinations may be responsible for host specificity.

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“…Vautor et al, (2008) has reported that the bap protein is a member of proteins playing a role in biofilm formation in many bacteria and they share common structural features as they have a high molecular weight and contain a core domain of tandem…”

Section: Introductionmentioning

confidence: 99%

Genotypic and Phenotypic Characterization of Clinical Isolates of Staphylococcus Aureus for Biofilm Formation Ability

Goyal¹

2014

Adv. Anim. Vet. Sci.

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The objective of the study was to characterize Staphylococcus aureus (S. aureus) isolated from human and animal clinical cases for their biofilm formation ability by genotypic and phenotypic methods. A total of 130 S. aureus strains isolated from human wound (n = 20), animal wound (n = 70) and animal mastitis (n = 40) cases were subjected to screening for 3 different biofilm associated genes (bap, icaA and icaD) and for phenotypic assessment for biofilm formation using Congo red agar, modified Congo red agar and microtitre plate assay. PCR assays were standardized for the detection of bap, icaA and icaD genes. The results indicated that icaA gene was present in 51.15% of the isolates and bap gene was present in 8.46% isolates. None of the isolates were positive for icaD gene. Human isolates (65%) had higher occurrence of icaA gene in comparison to animal isolates (49.09%). Dog wound isolates had higher occurrence of bap gene. Of the 3 methods used for phenotypic expression of biofilm by S. aureus isolates modified Congo red agar method showed 86.92% isolates to be positive, whereas by Congo red agar method only 63.07% S. aureus were found to be biofilm producer. Microtitre plate assay showed 75.38% S. aureus isolates to be biofilm producers. A good correlation was observed between genotypic and phenotypic biofilm formation ability of the isolates. Bap gene contained isolates showed higher biofilm producing ability compare to icaA gene harbored isolates.All copyrights reserved to Nexus® academic publishers

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Evaluation of the presence of the bap gene in Staphylococcus aureus isolates recovered from human and animals species

Cited by 67 publications

References 13 publications

Biofilm formation of Brazilian meticillin-resistant Staphylococcus aureus strains: prevalence of biofilm determinants and clonal profiles

Biofilm formation of Brazilian meticillin-resistant Staphylococcus aureus strains: prevalence of biofilm determinants and clonal profiles

Staphylococcus aureus host specificity: comparative genomics of human versus animal isolates by multi-strain microarray

Genotypic and Phenotypic Characterization of Clinical Isolates of Staphylococcus Aureus for Biofilm Formation Ability

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