Evaluation of the “Prostate test” in Checking the Administration of Oestrogens in the Calf1

Abstract

Summary The effect of various oestrogens was studied in male calves. The following oestrogens were used: diethylstilboestrol (in dosages of 20, 50 and 80 mg.), hexoestrol (80 and 160 mg.), dienoestrol (80 mg.) and oestradiol monobenzoate (80 mg.). The histological reaction of the prostate was studied in short term experiments (1–21 days after administration of the oestrogen) and long term experiments (8, 9, and 12 weeks after administration of the oestrogen). The oestrogens were injected both intramuscularly and subcutaneously. In the prostates of all treated animals, hyperplastic and metaplastic changes were observed. These changes were not influenced by the simultaneous application of a weak androgen (Laurabolin). Besides the parenteral administration the influence of orally administered diethylstilboestrol on the prostate was studied. Again the prostate reacted with hyperplasia and metaplasia. Zusammenfassung Auswertung des “Prostata‐Tests” beim Nachweis der Oestrogenapplikation an Kälbern Bei Stierkälbern wurde die Wirkung verschiedener Oestrogene untersucht. Folgende Oestrogene wurden geprüft: Diäthylstilböstrol (Dosis: 20, 50, 80 mg), Hexöstrol (80, 160 mg), Dienöstrol (80 mg) und Oestradiolmonobenzoat (80 mg). Die histologischen Reaktionen der Prostata wurden in kurzdauernden (1–21 Tage) sowie langdauernden (8, 9 und 12 Wochen) Experimenten studiert. Die Oestrogene wurden sowohl intramuskulär als auch subkutan appliziert. Bei allen Tieren traten hyperplastische und metaplastische Veränderungen auf. Diese Veränderungen wurden durch die gleichzeitige Verabreichung eines schwachen Androgens (Laurabolin) nicht beeinflußt. Außer der parenteralen Verabreichung wurde auch der Einfluß der oralen Applikation von Diäthylstilböstrol auf die Prostata geprüft. Auch in diesen Versuchen reagierte die Prostata mit einer Hyperplasie und Metaplasie. Résumé Evaluation du <test de la Prostate> après administration d'oestrogènes chez le veau On étudie les effets de différents oestrogènes sur des veaux mâles. On emploie les oestrogènes suivants: diéthylstilboestrol (en doses de 20, 50 et 80 mg), hexoestrol (80 et 160 mg), diènoestrol (80 mg) et le monobenzoate d'oestradiol (80 mg). On suit la réaction histologique de la prostate dans des expériences à court terme (1–21 jours après administration de l'oestrogène) et à long terme (8, 9 et 12 semaines après administration de l'oestrogène). On injecte les oestrogènes par voie intramusculaire et sous‐cutanée. On observe des modifications hyperplastiques et métaplasiques dans la prostate de tous les animaux traités. Ces modifications ne sont pas influencées par l'application simultanée d'un androgène faible (Laurabolin). Après étude des effets de l'administration parentérale, on étudie l'influence du diethylstilboestrol administré per os sur la prostate. La prostate réagit également par und hyperplasie et une métaplasie. Resumen Evaluación de la <Prueba Prostatica> en la búsqueda de la administración de estrógenos a terneros Estudióse el efecto de varios estrógenos en terneros. Se us...

Oestrogen‐induced Changes in the Genital Tract of the Male Calf

“…After both parenteral and oral administration of oestrogens hyperplastic and metaplastic changes of the prostate were found. These changes have already been described in earlier papers (RUITENBERG et al, 1969(RUITENBERG et al, , 1970KROES, 1970).…”

Progesterone receptor gene expression in the accessory sex glands of veal calves

“…The present study examined a novel detection strategy based on PR expression in the male accessory sex glands in response to 17β-oestradiol administration to reveal growth promoter abuse. In this study, besides inducing typical epithelial hyperplastic and metaplastic changes observed in the prostate, urethra and bulbourethral gland (Ruitenberg and others 1970, Kroes and others 1971, Hendry and others 1985) and testis atrophy (Noorafshan and others 2005), 17β-oestradiol induced significant transcriptional upregulation of PR cDNA in the prostate (7.5- and 16-fold increases, respectively) and particularly in the bulbourethral gland (39- and 429-fold increases, respectively) in the animals from groups A and B. These results demonstrate that 17β-oestradiol specifically induced transcriptional regulation of PR cDNA in the prostate and bulbourethral glands of animals in groups A and B, while no changes in PR transcription were observed in these tissue types in animals from group C, which shows that PR is specifically upregulated by 17β-oestradiol in the prostate and the bulbourethral glands in a dose-dependent way.…”

Histological Changes in the Genital Tract of the Female Calf after the Administration of Diethylstilbestrol and Hexestrol

“…In earlier publications (RUITENBERG, KROES, BERKVENS, 1969, 1970 the histological examination of the prostate was described as a method for the detection of estrogen administered to male calves since the administration of estrogens to calves is forbidden by law in the Netherlands.…”