Evaluation of the protective immunogenicity of the N, P, M, NV and G proteins of infectious hematopoietic necrosis virus in rainbow trout Oncorhynchus mykiss using DNA vaccines

Transmission potential of infectious hematopoietic necrosis virus in APEX-IHN®-vaccinated Atlantic salmon

“…Previous APEX-IHN ® efficacy studies suggested similar (50%) seroconversion rates up to 12 mo, but by 17 mo postvaccination, no neutralizing antibody titers were detected (Salonius et al 2007). While it is unclear whether such reduced antibody titers would equate to reduced vaccine efficacy, declines in vaccine efficacy post-vaccination have been demonstrated for similar IHNV and related aquatic rhabdoviral DNA vaccines (Corbeil et al 1999, Lorenzen et al 2000, McLauchlan et al 2003, Kurath et al 2006. The typical production cycle of farmed Atlantic salmon in BC is 18 mo, and although IHNV outbreaks at farms predominantly affected fish within the first 12 mo, outbreaks in older harvest size fish suggests their susceptibility to disease.…”

“…Consequently, there was a need for effective vaccines to prevent and control IHN disease to ensure industry sustainability. Research studies have demonstrated that a single inoculation of an IHNV DNA vaccine encoding the G protein provides protection against IHN disease in rainbow trout , numerous species of Pacific salmon (LaPatra et al 1989, Corbeil et al 1999, Garver et al 2005b, and Atlantic salmon (Traxler et al 1999). Moreover, the protective immunity afforded by the DNA vaccine is long-lived with limited plasmid persistence and biodistribution (Garver et al 2005a, Kurath et al 2006), making it a favorable candidate for commercial applications.…”

Responses of cloned rainbow trout Oncorhynchus mykiss to an attenuated strain of infectious hematopoietic necrosis virus

“…The fusion protein induced an immuno-protective response in fish. While it is tempting to speculate that a portion of the neutralizing activity detected in these sera might be due to reaction with the 90 kDa protein or M1, recent results contributed by Corbeil et al (1999) and work by Engelking & Leong (1989) suggest that this is not the case. However, Lee et al (1996) found that MAb AB7, which identifies the unique 90 kDa stress protein derived from CHSE 214 cells, was by itself able to neutralize a Korean isolate (PRT) of IHNV in vitro.…”

The Protective Effect of Recombinant Glycoprotein Vaccine Against HIRRV Infection.

“…It has also been reported that an antigenic determinant of the glycoprotein gene of IHNV expressed as fusion protein with trpE protein of Escheri chia coli induced protective immunity (Gilmore et al, 1988). Recent studies on DNA vaccines for fish rhab dovirus glycoprotein also shown that the glycoprotein is sufficient to induce protective immunity against the cor responding virus and even other rhabdoviruses in the same genus (Corbeil et al, 1999;Kim et al, 2000).…”

“…However, a recent development in molecular biology has made it possible to produce a large amount of recombinant viral protein as a sub-unit vaccine that can be used for vacci nation by immersion or injection of individual fish (Munn, 1994;Lorenzen, 1999). In addition, viral genes cloned into an expression vector and introduced into fish tissue as DNA vaccines have been proved to be effective in in door experiments and small-scale field experiments (Corbeil et al, 1999;Kim et al, 2000).…”