Evaluation of the quality consistency of powdered poppy capsule extractive by an averagely linear‐quantified fingerprint method in combination with antioxidant activities and two compounds analyses

Abstract

A novel averagely linear-quantified fingerprint method was proposed and successfully applied to monitor the quality consistency of alkaloids in powdered poppy capsule extractive. Averagely linear-quantified fingerprint method provided accurate qualitative and quantitative similarities for chromatographic fingerprints of Chinese herbal medicines. The stability and operability of the averagely linear-quantified fingerprint method were verified by the parameter r. The average linear qualitative similarity SL (improved based on conventional qualitative "Similarity") was used as a qualitative criterion in the averagely linear-quantified fingerprint method, and the average linear quantitative similarity PL was introduced as a quantitative one. PL was able to identify the difference in the content of all the chemical components. In addition, PL was found to be highly correlated to the contents of two alkaloid compounds (morphine and codeine). A simple flow injection analysis was developed for the determination of antioxidant capacity in Chinese Herbal Medicines, which was based on the scavenging of 2,2-diphenyl-1-picrylhydrazyl radical by antioxidants. The fingerprint-efficacy relationship linking chromatographic fingerprints and antioxidant activities was investigated utilizing orthogonal projection to latent structures method, which provided important pharmacodynamic information for Chinese herbal medicines quality control. In summary, quantitative fingerprinting based on averagely linear-quantified fingerprint method can be applied for monitoring the quality consistency of Chinese herbal medicines, and the constructed orthogonal projection to latent structures model is particularly suitable for investigating the fingerprint-efficacy relationship.

Recent advances of novel technologies for quality consistency assessment of natural herbal medicines and preparations

“…Therefore, by employing 18 luciferase reporter cell lines and two enzyme assays based on the mechanism of PHY906, a mechanism-based quality control platform Recently, researchers have proposed a new average linear quantitative fingerprinting method, combined with liquid phase analysis of antioxidant activity, successfully applied to the quality consistency evaluation of two herbal preparations. The relationship between the efficacy relationship of fingerprints and antioxidant activity was studied by orthogonal projection to latent structures method, which provided important pharmacodynamic information for quality control of traditional Chinese medicine [137,138].…”

Interpol review of controlled substances 2016–2019

“…Opium/Opium Poppy/Poppy Seeds/Papaver Somniferum (see also Papaver below, and Opiates in Subsection 1.C ): 2016 opium profiling based on the relative content of five principal and 14 minor opium alkaloids using UPLC-Q-TOF to trace clandestine opium production and trafficking [ 797 ]; 2017 Forensic application of EST-derived STR markers in opium poppy to distinguish cultivars [ 798 ]; morphine content in the poppy straw of edible poppy (Papaver somniferum L.) [ 799 ]; novel fingerprint method applied to monitor the quality consistency of alkaloids in powdered poppy capsule extractive [ 800 ]; determination of the content of morphine, codeine, and papaverine in ornamental P. somniferum cultivars by HPLC and CE [ 801 ]; identification of illicit opium cultivation using SERS with microfluidics for the detection of papaverine at low concentrations [ 802 ]; isolation and characterization of a novel O-methyltransferase (OMT) involved in the biosynthesis of alkaloids in the California poppy [ 803 ]; SFE of papaverine and noscapine from poppy capsules [ 804 ]; 2018 study to examine relationships between capsule yield, some other yield and quality traits by means of correlation, path and principal components analysis in fifteen opium poppy (Papaver somniferum L.) cultivars registered in Turkey [ 805 ]; LC-MS/MS method to determine the content of six opium alkaloids (morphine, codeine, thebaine, noscapine, papaverine and narceine) in poppy seeds and bakery products with a limit of quantification (LOQ) of 0.1 mg/kg [ 806 ]; quantification of Morphine, codeine, and thebaine in home-brewed Poppy Seed tea extracts by LC-MS/MS [ 807 ]; examination of biosynthesis pathways and morphine content in opium poppy cultivars [ 808 ]; use of deep learning target detection to identify the location of poppy parcels and map their spatial distribution [ 809 ]; GC-MS method for the characterization of thebaol, a component of opium poppy [ 810 ]; bulk analysis of aged oil and juglet used for poppy distribution by GC-EI-MS and pyGC-EI-MS and analysis of the alkaloid extracts by HPLC-ESI-MS using both triple quadrupole and FTICR mass spectrometer [ 811 ]; 2019 review [ 812 ];…”

UHPLC-ESI-Q-TOF-MS/MS analysis, antioxidant activity combined fingerprints for quality consistency evaluation of compound liquorice tablets

“…5D) of the calibration model, 38 peaks (2-5, 8, 10, 12, 14, 17, 19, 20, 23-26, 28, 30, 31, 34, 36, 41, 43, 44, 46, 48-54, 56, 57, 61-64, and 70) out of 70 ngerprints in the fusion chromatogram were positively correlated, while the remaining peaks were negatively correlated with SER, indicating that the majority of the chemical components in the CLT samples possessed antioxidant abilities. Furthermore, the peaks 20,25,26,28,31,36,41,51, and 54 in the fusion ngerprints were identied as MPE, LQA, LQT, CON, SMB, ISS, LQG, ISG, and GLA, respectively, by comparing the retention time and the online UV spectra with reference standards.…”

“…In this study, both the averaged linear qualitative and averaged quantitative similarities of the ngerprints of the CLT samples were assessed by the averaged linear quantied ngerprint method (ALQFM), which was developed and successfully applied to address the issue of performing a qualitative and quantitative comparison of the reference standards and PPCE samples. 25 CLT is an important TCM prepared from ve medicinal herbs, including licorice root (extract) concrete (LRC), powdered poppy capsule extractive (PPCE), camphor, oleum anisi stellati, and sodium benzoic (SMB), which have been widely used for anti-inammatory, antitussive, expectorant, and antiasthmatic actions. 26 The compositions of CLT mainly include avonoids, saponins, alkaloids, and other compounds, as reported in previous reports, [27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44] and these chemical components have a common pharmacological activity, that is, their antioxidant activity.…”