Evaluation of the RapID CB Plus System for Identification of Coryneform Bacteria and<i>Listeria</i>spp

Funke, Guido; Peters, K.; Aravena-Román, Max

doi:10.1128/jcm.36.9.2439-2442.1998

Cited by 40 publications

(7 citation statements)

References 9 publications

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“…RapID CB Plus system is composed of 4 carbohydrate utilization tests and 15 enzymatic tests and identifies 23 Corynebacterium species (Funke et al . ). Evaluation of these two systems revealed that 97·6 and 95% of strains, respectively, were identified correctly (Freney et al .…”

Section: Manual Biochemical Identification Systemsmentioning

confidence: 97%

“…6 standard for API Coryne system), hence, it is often impossible to identify coryneform bacteria directly from primary growth plates (Funke et al . ).…”

Section: Manual Biochemical Identification Systemsmentioning

confidence: 97%

“…Funke et al . () noticed that reading of the RapID CB Plus enzymatic reactions is sometimes more difficult compared to reading of the API Coryne system reactions due to the occurrence of pale shades. Reactions for fermentation of carbohydrates are sometimes difficult to read in both systems.…”

Section: Manual Biochemical Identification Systemsmentioning

confidence: 99%

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Contemporary microbiology and identification ofCorynebacteriaspp. causing infections in human

Zasada

Mosiej

2018

Lett Appl Microbiol

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Significance and Impact of the Study: The number of opportunistic infections caused by Corynebacteria is increasing due to increase in number of immunocompromised patients. New Corynebacterium species and new human infections, caused by this group of bacteria, has been described recently. However, identification of Corynebacteria is still a challenge despite application of sophisticated laboratory methods. In the study we present possibilities and limitations of various commercial systems for identification of Corynebacteria. AbstractThe Corynebacterium is a genus of bacteria of growing clinical importance. Progress in medicine results in growing population of immunocompromised patients and growing number of infections caused by opportunistic pathogens. A new infections caused by new Corynebacterium species and species previously regarded as commensal micro-organisms have been described. Parallel with changes in Corynebacteria infections, the microbiological laboratory diagnostic possibilities are changing. But identification of this group of bacteria to the species level remains difficult. In the paper, we present various manual, semiautomated and automated assays used in clinical laboratories for Corynebacterium identification, such as API Coryne, RapID CB Plus, BBL Crystal Gram Positive ID System, MICRONAUT-RPO, VITEK 2, BD Phoenix System, Sherlock Microbial ID System, MicroSeq Microbial Identification System, Biolog Microbial Identification Systems, MALDI-TOF MS systems, polymerase chain reaction (PCR)-based and sequencing-based assays. The presented assays are based on various properties, like biochemical tests, specific DNA sequences, composition of cellular fatty acids, protein profiles and have specific limitations.

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Section: Manual Biochemical Identification Systemsmentioning

confidence: 97%

“…6 standard for API Coryne system), hence, it is often impossible to identify coryneform bacteria directly from primary growth plates (Funke et al . ).…”

Section: Manual Biochemical Identification Systemsmentioning

confidence: 97%

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Contemporary microbiology and identification ofCorynebacteriaspp. causing infections in human

Zasada

Mosiej

2018

Lett Appl Microbiol

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“…In the API-Coryne system (bioMérieux) all three yield the code 2100004; thus, additional tests (CAMP, DNase, APIZYM reactions) are required. The VITEK 2 ANC card (bio-Mérieux) most likely also requires additional tests [18]. The RapID CB Plus system (Remel, Inc., Lenexa, KS, USA) correctly identified T. otitidis and C. auris but not C. afermentans to the species level [17].…”

Section: Laboratory Diagnosismentioning

confidence: 99%

Turicella otitidis and Corynebacterium auris: 20 years on

Graevenitz¹,

Funke

2013

Infection

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Turicella otitidis and Corynebacterium auris, described as new species 20 years ago, have been isolated mainly from the external ear canal and middle ear fluid. While their taxonomic position has been clearly established, their diagnosis in the routine laboratory is difficult. The question of their pathogenic potential in otitis is still open but might be elucidated better if corynebacteria are speciated more often.

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“…Kligler's or triple sugar iron agar should also be inoculated. In lieu of single tests, commercial identification systems such as API Coryne v.2 [22] or RapID CB Plus (Remel Inc., Norcross, GA, USA) [23] may be used; both contain R. dentocariosa in their data bases. Directions for use must be followed strictly.…”

Section: D E N T I F I C a T I O N O F R D E N T O C A R I O S A mentioning

confidence: 99%