Evaluation of the Rapid Polymyxin NP test for detection of colistin susceptibility in Enterobacteriaceae isolated from Thai patients

Yainoy, Sakda; Hiranphan, Monchanok; Phuadraksa, Thanawat; Eiamphungporn, Warawan; Tiengrim, Surapee; Thamlikitkul, Visanu

doi:10.1016/j.diagmicrobio.2018.05.009

Cited by 14 publications

(6 citation statements)

References 17 publications

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“…The inhouse Rapid Polymyxin NP test is prepared with stock solutions of polymyxins and a Rapid Polymyxin NP solution, which consists of cation-adjusted Mueller-Hinton broth powder, phenol red indicator and d(+)-glucose (Nordmann et al, 2016a). This test has demonstrated an excellent detection of colistin resistance in several studies, including detecting colistin resistance directly from blood cultures with a sensitivity of 100% (Jayol et al, 2016;Malli et al, 2019;Yainoy et al, 2018). However, in a study using ComASP as the reference, the Rapid Polymyxin NP test had a lower specificity than BD Phoenix, Vitek 2 and E-test with K. pneumoniae isolates (Table 1) (Malli et al, 2018).…”

Section: Rapid Polymyxin Npmentioning

confidence: 99%

“…The Rapid Polymyxin NP is a colorimetric test that is based on glucose metabolism to detect the growth of Enterobacterales at a given concentration of a polymyxin (colistin or polymyxin B) (Nordmann et al, 2016a). Resistance to polymyxins is demonstrated by a colour change (orange to yellow) of a pH indicator, that is, phenol red, as a result of acid formation associated with the metabolism of glucose (Nordmann et al, 2016a; Poirel et al, 2018; Yainoy et al, 2018). Rapid Polymyxin NP test is commercially available (ELITechGroup) and can also be performed in‐house with the preparation of two solutions (Jayol, Kieffer, et al, 2018; Nordmann et al, 2016a).…”

Section: Phenotypic Testsmentioning

confidence: 99%

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Current and emerging polymyxin resistance diagnostics: A systematic review of established and novel detection methods

Leshaba

Mbelle

Sekyere

2021

J of Applied Microbiology

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Tweet: "Colistin resistance is a global threat to bacterial infections' management. Being a last-resort antibiotic for treating of MDR Gram-negative infections, it is important to establish an efficient diagnostic method for routine use in clinical microbiology laboratories. Herein, current & emerging diagnostics for detecting colistin resistance are described to inform clinical diagnostic options." Author summary/highlights/importance  Polymyxin resistance is rapidly increasing, threatening public and veterinary healthcare.  As one of the last-line antibiotics, polymyxin must be conserved by containing the spread of polymyxin resistance.  Detecting colistin resistance relies on determining colistin MIC values by standard broth microdilution, which is labour-intensive with longer turnaround time (TAT).

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Section: Rapid Polymyxin Npmentioning

confidence: 99%

Section: Phenotypic Testsmentioning

confidence: 99%

Current and emerging polymyxin resistance diagnostics: A systematic review of established and novel detection methods

Leshaba

Mbelle

Sekyere

2021

J of Applied Microbiology

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“…Resistance to polymyxins is demonstrated by a colour change (orange to yellow) of a pH indicator, i.e. phenol red, as a result of acid formation associated with the metabolism of glucose 11, 39, 40 . Rapid polymyxin NP test is commercially available (ELITechGroup, Puteaux, France) and can also be performed in-house with the preparation of two solutions 11, 41 .…”

Section: Phenotypic Testsmentioning

confidence: 99%

“…The in-house rapid polymyxin NP test is prepared with stock solutions of polymyxins and a rapid polymyxin NP solution, which consists of cation-adjusted Mueller-Hinton broth powder, phenol red indicator and D(+)-glucose 11 . This test has demonstrated an excellent detection of colistin resistance in several studies, including detecting colistin resistance directly from blood cultures with a sensitivity of 100% 40, 42, 43 . However, in a study using ComASP as the reference, the Rapid Polymyxin NP test had a lower specificity than BD Phoenix, Vitek 2 and E-test with K. pneumoniae isolates (Table 1) 36 .…”

Section: Phenotypic Testsmentioning

confidence: 99%

Current and emerging polymyxin resistance diagnostics: a systematic review of established and novel detection methods

Leshaba¹,

Mbelle²,

Sekyere³

2020

Preprint

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Background. The escalation of colistin resistance, due to transferable mcr-genes, threatens public and animal health as there are limited therapeutic options. Given that colistin is a last-line antibiotic, there is a need to contain the spread of its resistance to conserve its efficacy. Herein, current and emerging colistin resistance diagnostics are described to inform faster clinical diagnostic choices. Methods. A literature search in Pubmed, between 2016 and 2020, was performed. English articles evaluating colistin resistance methods/diagnostics were included. Results. Screening resulted in the inclusion of 89 journal articles. Current colistin resistance diagnostics are either phenotypic or molecular. Broth microdilution (BMD) is currently the only golden standard for determining colistin MIC values. However, other methods have been developed to overcome the challenges associated with the BMD. Phenotypic methods comprise agar-based methods i.e. ChromAgar Col-APSE, SuperPolymyxin, ChromID Colistin R etc., MIC-determiners i.e. Microscan, BD Phoenix, Sensitest colistin, Sensititre, Micronaut etc., MCR-detectors i.e. lateral flow immunoassay (LFI), chelator based assays i.e. EDTA- and DPA-based tests as well as biochemical colorimetric tests i.e. Rapid Polymyxin NP test, Rapid ResaPolymyxin NP test etc. Molecular methods only characterize mobile colistin resistance; they include PCR, LAMP, whole-genome sequencing (WGS) etc. Conclusion. Considering the efficiency (sensitivity, specificity, turnaround time) of the Rapid ResaPolymyxin NP test, it is recommended for the initial screening of colistin-resistant isolates. This can be followed by chelator-based tests or the LFI for the rapid screening of mcr-genes. However molecular assays such as LAMP, PCR may be considered for use in well-equipped clinical laboratories.

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“…Numerous analytical approaches based on microbiological, [4][5][6] capillary electrophoretic methods, 7 and spectroscopic (spectrophotometric 8 and uorimetric methods 9 ) assays were reported. Moreover, published chromatographic techniques such as liquid chromatography (LC) [10][11][12][13][14][15][16] and high-performance liquid chromatography (HPLC) [17][18][19][20][21] were carefully collected for analysis of CS.…”

Section: Introductionmentioning

confidence: 99%

New validated spectrofluorimetric protocol for colistin assay through condensation with 2,2-dihydroxyindan-1,3-dione: application to content uniformity testing

et al. 2022

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Evaluation of the Rapid Polymyxin NP test for detection of colistin susceptibility in Enterobacteriaceae isolated from Thai patients

Cited by 14 publications

References 17 publications

Current and emerging polymyxin resistance diagnostics: A systematic review of established and novel detection methods

Current and emerging polymyxin resistance diagnostics: A systematic review of established and novel detection methods

Current and emerging polymyxin resistance diagnostics: a systematic review of established and novel detection methods

New validated spectrofluorimetric protocol for colistin assay through condensation with 2,2-dihydroxyindan-1,3-dione: application to content uniformity testing

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