Evaluation of the RapID yeast plus system for the identification of yeast

Moghaddas, Jamshid; Truant, Allan L.; Jordan, Carol A.; Buckley, Helen R.

doi:10.1016/s0732-8893(99)00083-8

Cited by 6 publications

(3 citation statements)

References 6 publications

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“…The data obtained in this study were similar to those from previous reports (2,6,9,12,15,(17)(18)20) showing that the VITEK 2 and RapID Yeast Plus systems were comparable to other commercial methods (e.g., API 20C AUX and ID 32C) in their abilities to correctly identify yeast species. The VITEK 2 colorimetric YST card was recently developed to replace the older fluorimetric ID-YST card, yet a recent study conducted by Loïez et al (15) on 172 clinical yeast isolates showed that 161 (93.6%) and 144 (83.7%) isolates were correctly identified with the ID-YST and YST cards, respectively.…”

supporting

confidence: 90%

Evaluation of VITEK 2 and RapID Yeast Plus Systems for Yeast Species Identification: Experience at a Large Clinical Microbiology Laboratory

et al. 2007

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A total of 750 clinical yeast isolates were evaluated by two identification systems, VITEK 2 and RapID Yeast Plus, using sequence analysis of the rRNA gene internal transcribed spacer regions as the reference method. The VITEK 2 and RapID systems correctly identified 737 (98.2%) and 716 (95.5%) isolates, respectively.Although Candida species, including the pathogenic Candida albicans, remain the yeast species most commonly encountered in a clinical microbiology laboratory, a variety of other yeasts are recovered from patients with well-documented infections. Accurate identification of these species is clinically important, as certain yeast species are associated with specific diseases (11,22). In addition, yeast species can differ greatly in their relative virulence levels (22) as well as their susceptibilities to antifungal agents (19). While there is an increasing move toward molecular biology-based diagnostic approaches (1), identification of clinical yeast isolates is still typically performed by biochemical, morphological, and physiological tests (8). These phenotypic systems often produce results that may not be accurate, so their performance must be reassessed to enable users to have a reliable system for yeast identification. In this study, we compared the VITEK 2 (bioMérieux VITEK, Marcy l'Etoile, France) and RapID Yeast Plus (Remel Inc., Lenexa, KS) systems, with sequence analysis of the rRNA gene internal transcribed spacer (ITS) regions used as the reference method, for the identification of medically important yeasts typically found in a large clinical microbiology laboratory.A total of 750 yeast isolates, representing 24 species of six genera, were studied. Isolates were obtained from clinical samples (oral, vaginal, anorectal, urine, stool, blood, central Candida krusei ATCC 6258 were used as quality control strains. Isolates were grown on Sabouraud dextrose agar (Kima, Padua, Italy) for 48 h at 30°C prior to being tested. Each isolate was simultaneously tested with the VITEK 2 system (version 4.02), using the new colorimetric YST card (BioMérieux), and with the RapID Yeast Plus system (version 1.95), according to the instructions of their respective manufacturers, and the results were compared. In cases of discrepant results, both methods were repeated and the results for the second runs were accepted as the final results. In cases of identification with low discrimination (see below), additional tests (e.g., microscopic morphology on cornmeal-Tween 80 agar or growth at 42 to 45°C) were carried out (3). ITS sequence analysis was performed on all 750 isolates and used as the reference system. Thus, purified genomic DNA was obtained from each yeast isolate, using an EZ1 DNA tissue kit (QIAGEN, Milan, Italy) and a BioRobot EZ1 workstation (QIAGEN) in accordance with the manufacturer's instructions. This included a preliminary step in which yeast colonies were resuspended in 190 l of buffer G2, 10 l of lyticase (25 units/l) was added to each cell sample, and the resulting mixture was incubated at 3...

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confidence: 90%

Evaluation of VITEK 2 and RapID Yeast Plus Systems for Yeast Species Identification: Experience at a Large Clinical Microbiology Laboratory

et al. 2007

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“…Commercial identification kits are faster, simpler to perform, and do not require special equipment. On the other hand, they rely on only a few tests, limiting their application in identifying environmental strains, although their usefulness for clinical isolates has been reported (8,12,20).…”

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confidence: 99%

“…This conventional methodology requires the evaluation of some 60 to 90 tests, resulting in a complex, laborious, and time-consuming process. In recent years, rapid kit identification methods have been developed to overcome the complexity of traditional methods (8,20,28). One of these methods, the API 20C AUX system (bioMèrieux, Lyon, France), has been widely used and consists of 19 assimilation tests.…”

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confidence: 99%

Yeast Species Associated with Orange Juice: Evaluation of Different Identification Methods

Arias

Burns

Friedrich

et al. 2002

Appl Environ Microbiol

133

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Five different methods were used to identify yeast isolates from a variety of citrus juice sources. A total of 99 strains, including reference strains, were identified using a partial sequence of the 26S rRNA gene, restriction pattern analysis of the internal transcribed spacer region (5.8S-ITS), classical methodology, the RapID Yeast Plus system, and API 20C AUX. Twenty-three different species were identified representing 11 different genera. Distribution of the species was considerably different depending on the type of sample. Fourteen different species were identified from pasteurized single-strength orange juice that had been contaminated after pasteurization (PSOJ), while only six species were isolated from fresh-squeezed, unpasteurized orange juice (FSOJ). Among PSOJ isolates, Candida intermedia and Candida parapsilosis were the predominant species. Hanseniaspora occidentalis and Hanseniaspora uvarum represented up to 73% of total FSOJ isolates. Partial sequence of the 26S rRNA gene yielded the best results in terms of correct identification, followed by classical techniques and 5.8S-ITS analysis. The commercial identification kits RapID Yeast Plus system and API 20C AUX were able to correctly identify only 35 and 13% of the isolates, respectively. Six new 5.8S-ITS profiles were described, corresponding to Clavispora lusitaniae, Geotrichum citri-aurantii, H. occidentalis, H. vineae, Pichia fermentans, and Saccharomycopsis crataegensis. With the addition of these new profiles to the existing database, the use of 5.8S-ITS sequence became the best tool for rapid and accurate identification of yeast isolates from orange juice.

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Commercial Methods for Identification and Susceptibility Testing of Fungi

Moser

Wicker

2016

Manual of Commercial Methods in Clinical Microbiology

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Evaluation of the RapID yeast plus system for the identification of yeast

Cited by 6 publications

References 6 publications

Evaluation of VITEK 2 and RapID Yeast Plus Systems for Yeast Species Identification: Experience at a Large Clinical Microbiology Laboratory

Evaluation of VITEK 2 and RapID Yeast Plus Systems for Yeast Species Identification: Experience at a Large Clinical Microbiology Laboratory

Yeast Species Associated with Orange Juice: Evaluation of Different Identification Methods

Commercial Methods for Identification and Susceptibility Testing of Fungi

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