Evaluation of the Roche Septi-Chek AFB system for recovery of mycobacteria

D'Amato, R F; Isenberg, Henry D.; Hochstein, Lisa; Mastellone, Anthony J.; Alperstein, Phyllis

doi:10.1128/jcm.29.12.2906-2908.1991

Cited by 30 publications

(14 citation statements)

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“…Published contamination rates range from 0.3 to 8.9% for the Septi-Chek AFB system (1,5,6,11,13,20,23) and 0.4 to 41% for LJ (5,6,11,13,15,20,23,24). In our study the contamination rates were 7.6 and 6.5% for the Septi-Chek AFB system and LJ, respectively.…”

Section: Discussionmentioning

confidence: 41%

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Comparison of fluorescent BACTEC 9000 MB system, Septi-Chek AFB system, and Lowenstein-Jensen medium for detection of mycobacteria

1996

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The newly developed fluorescent BACTEC 9000 MB system for automated culture of mycobacteria was compared with the Septi-Chek AFB system and Lowenstein-Jensen medium (LJ). A total of 2,005 clinical specimens were included in the study. Mycobacteria were isolated from 202 (10.1%) specimens, including 155 Mycobacterium tuberculosis complex isolates and 47 Mycobacteria other than M. tuberculosis isolates. Of 131 isolates detected by the BACTEC system, the Septi-Chek AFB system, or both, 120 (91.6%) were detected by the BACTEC system and 105 (80.2%) were detected by the Septi-Chek AFB system (P < 0.02). The recovery rate in the BACTEC system compared with that in the Septi-Chek AFB system was significantly higher for M. tuberculosis complex isolates (P < 0.005) and for isolates from acid-fast smear-negative specimens (P < 0.01). Of 148 isolates detected by the BACTEC system, LJ, or both, 142 (95.9%) were detected by the BACTEC system and 118 (79.9%) were detected by LJ (P < 0.001). The recovery rate in the BACTEC system compared with that on LJ was significantly higher for M. tuberculosis complex isolates (P < 0.001). The BACTEC system detected more mycobacteria from both smear-positive and smear-negative specimens than LJ. The mean times to detection of mycobacteria were 17.6 days for the BACTEC system, 26.0 days for the Septi-Chek AFB system, and 29.4 days for LJ. The BACTEC fluorescent 9000 MB system is a rapid, sensitive, and efficient method for the isolation of mycobacteria.

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Section: Discussionmentioning

confidence: 41%

“…The radiometric BACTEC 460 TB system (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md.) has been reported to significantly decrease the time required for detection (5,10,15,16,21,22). The use of radioisotopically labeled substrates has, however, inhibited universal application of this system.…”

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confidence: 99%

Comparison of fluorescent BACTEC 9000 MB system, Septi-Chek AFB system, and Lowenstein-Jensen medium for detection of mycobacteria

1996

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“…Another serious limitation of culture techniques is sensitivity. Although they are considered to be the ''gold standard'' against which all other methods are measured, their sensitivity leaves much room for improvement (5,9,11). Examination of direct smears of clinical specimens for acid-fast bacilli is rapid but lacks sensitivity and specificity.…”

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confidence: 99%

Rapid diagnosis of pulmonary tuberculosis by using Roche AMPLICOR Mycobacterium tuberculosis PCR test

et al. 1995

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A rapid PCR-based test for the diagnosis of pulmonary tuberculosis, the Roche AMPLICOR Mycobacterium tuberculosis test (AMPLICOR MTB), was evaluated. Results from AMPLICOR MTB were compared with culture results and the final clinical diagnosis for each patient. A total of 985 specimens from 372 patients were tested. When AMPLICOR MTB results were compared with resolved results, i.e., a specimen grew M. tuberculosis or was obtained from a patient with a clinical diagnosis of tuberculosis, the sensitivity, specificity, positive predictive value, and negative predictive value for the AMPLICOR MTB test were 66.7, 99.6, 91.7, and 97.7%, respectively. These results were comparable to those obtained from culture. Test results were available approximately 6.5 h after specimen receipt in the laboratory. Our data demonstrate that AMPLICOR MTB will provide rapid, valuable information for the diagnosis and control of tuberculosis.

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“…Sensitivity, selective power and early detection of growth are major parameters in the evaluation of culture media. The features of MGIT emerging from this study reveal a sensitivity very similar to that of the most widely applied solid medium, Löwenstein–Jensen, and to that of the thoroughly validated biphasic method, Septi‐Chek [12–16]. The lower rate of false‐negative results presented by MGIT in comparison with Septi‐Chek and Löwenstein–Jensen is in fact counterbalanced by a higher number of contaminations, a clue to its lower selective power.…”

Section: Discussionmentioning

confidence: 65%

Multicenter evaluation of mycobacteria growth indicator tube (MGIT) compared with the BACTEC radiometric method, BBL biphasic growth medium and Löwenstein—Jensen medium

Tortoli

Mandler

Tronci

et al. 1997

Clinical Microbiology and Infection

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OBJECTIVE: To evaluate the new BBL mycobacteria growth indicator tube (MGIT) in comparison with other media. METHODS: MGIT was evaluated in 10 Italian centers on 433 clinical samples, mainly of respiratory origin and mainly smear positive, in comparison with Löwenstein---Jensen and with one or more other methods represented, according to participating centers, by the BACTEC radiometric method or by the biphasic BBL Septi-Chek AFB system. While MGIT and Löwenstein---Jensen were used for all the samples, 285 of them were also inoculated in BACTEC vials and 274 in biphasic bottles. Of these samples, 132 were investigated with all the four methods. RESULTS: Although less rapid and sensitive than the radiometric method, the results of MGIT were equal when compared with the other two media with respect to overall isolation yield; furthermore, it allowed the detection of growth in significantly shorter times. CONCLUSIONS: The results of this study indicate the value of MGIT for the detection of mycobacteria and, thanks to its extreme simplicity of use, its suitability for small and large laboratories. Its combined use with a solid medium can substantially improve the diagnosis of mycobacterial infection.

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Evaluation of the Roche Septi-Chek AFB system for recovery of mycobacteria

Cited by 30 publications

References 1 publication

Comparison of fluorescent BACTEC 9000 MB system, Septi-Chek AFB system, and Lowenstein-Jensen medium for detection of mycobacteria

Comparison of fluorescent BACTEC 9000 MB system, Septi-Chek AFB system, and Lowenstein-Jensen medium for detection of mycobacteria

Rapid diagnosis of pulmonary tuberculosis by using Roche AMPLICOR Mycobacterium tuberculosis PCR test

Multicenter evaluation of mycobacteria growth indicator tube (MGIT) compared with the BACTEC radiometric method, BBL biphasic growth medium and Löwenstein—Jensen medium

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