Evaluation of the rodent micronucleus assay in the screening of IARC carcinogens (Groups 1, 2A and 2B)

Morita, Takeshi; Asano, Naoki; Awogi, Takumi; Sasaki, Yu; Sato, Seiichi; Shimada, Hiroyasu; Sutou, Shizuyo; Suzuki, Takayoshi; Wakata, Akihiro; Sofuni, Toshio; Hayashi, Makoto

doi:10.1016/s1383-5718(96)00070-8

Cited by 256 publications

(151 citation statements)

References 89 publications

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“…The in vivo rat micronuclei test and chromosome aberrations assay are two of the most frequently used and sensitive tests for investigating the genotoxic profile of chemicals, these tests having been recommended for routine analysis because they produce results that are considered highly relevant in the human context (Morita et al, 1997;Preston et al, 1987). The Copaifera duckei oleoresin analyzed by us was very rich in diterpene acids and possessed moderate amounts of sesquiterpene hydrocarbons, confirming the report by that there are differences in the chemical composition of the oleoresin produced by different Copaifera species.…”

Section: Discussionmentioning

confidence: 99%

In vivo evaluation of the mutagenic potential and phytochemical characterization of oleoresin from Copaifera duckei Dwyer

et al. 2005

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We characterized the chemical constituents of Copaifera duckei oleoresin and used dermal application to Wistar rats to evaluated its possible mutagenic and cytotoxic activities on peripheral blood reticulocytes and bone marrow cells. Chemical characterization of the oleoresin revealed the presence of sesquiterpene hydrocarbons, an unidentified neutral diterpene and diterpene acids. To evaluate mutagenicity evaluation the rats were treated with 10, 25 and 50% of the LD 50 dose of the oleoresin for three consecutive days and peripheral blood collected after 0, 24, 48 and 72 h for micronucleus analysis. The rats were humanly sacrificed 24 hours after the last treatment and chromosome preparations made using standard techniques. At the three concentrations and the three time intervals tested we found that there were no statistically significant differences in either the mean number of micronucleated reticulocytes (MNRETs) or the number of chromosomal aberrations as to the negative control. However, at 25 and 50% of the LD 50 dose of the oleoresin there was a significant decrease in the mitotic index (MI) as compared to the negative control. Under our experimental conditions, C. duckei V11 oleoresin produced no mutagenic effects on bone marrow cells or in peripheral reticulocytes as assessed by chromosome aberrations and the micronucleus test respectively, but showed cytotoxic activity at high doses.

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Section: Discussionmentioning

confidence: 99%

In vivo evaluation of the mutagenic potential and phytochemical characterization of oleoresin from Copaifera duckei Dwyer

et al. 2005

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“…These modifications offer many advantages to the conventional bone marrow assay and are widely used to evaluate chemical clastogenicity (Morita et al, 1997).…”

mentioning

confidence: 99%

Anticlastogenic Effect of Eryngium foetidum L. Assessed by Erythrocyte Micronucleus Assay

Promkum¹,

Butryee²,

Tuntipopipat³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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“…The mouse micronucleus assay has been used to evaluate aneuploidy and clastogenic chromosome aberrations (Morita et al, 1997), but experiments using erythrocytes from mammalian species other than mice (e.g. humans, laboratory rats and wild rodents) have met with less success due to the ability of the spleen to remove micronucleated (MN) erythrocytes from the blood (Simula and Priestly, 1992;Holden et al, 1997).…”

Section: Resultsmentioning

confidence: 99%

Clastogenicity of Piper cubeba (Piperaceae) seed extract in an in vivo mammalian cell system

et al. 2007

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The plant Piper cubeba is widely distributed in tropical and subtropical regions and is used medically for various purposes but has not yet been evaluated for genotoxicity. We used male and female Swiss mice and Wistar rats and the comet assay and micronucleus test to investigate the mutagenic potential of a crude extract of P. cubeba seeds. The rodents were administered 0.5 g kg -1 , 1.0 g kg -1 and 1.5 g kg -1 of the extract by gavage. For the Swiss mice, peripheral blood was collected 24 h after treatment for the comet assay, and at 48 and 72 h for the micronucleus test. For the Wistar rats, peripheral blood and hepatic cells were collected for the comet assay and bone marrow cells were collected for the micronucleus test 24 h after treatment. At 1.5 g kg -1 , the highest dose tested, the extract induced a statistically significant increase in both the mean number of micronucleated polychromatic erythrocytes and the level of DNA damage in the rodent cell types analyzed. Under our experimental conditions, the P. cubeba seed extract was genotoxic in vivo when administered orally to mice and rats.

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Evaluation of the rodent micronucleus assay in the screening of IARC carcinogens (Groups 1, 2A and 2B)

Cited by 256 publications

References 89 publications

In vivo evaluation of the mutagenic potential and phytochemical characterization of oleoresin from Copaifera duckei Dwyer

In vivo evaluation of the mutagenic potential and phytochemical characterization of oleoresin from Copaifera duckei Dwyer

Anticlastogenic Effect of Eryngium foetidum L. Assessed by Erythrocyte Micronucleus Assay

Clastogenicity of Piper cubeba (Piperaceae) seed extract in an in vivo mammalian cell system

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