Evaluation of the role of HIS-1 and HSP-70 genes in pathology and response to the drugs by antisense oligonucleotides in Leishmania major

Abstract

Propose Leishmaniasis is a protozoan disease that caused by Leishmania spp. with wide spectrum of manifestations. Researchers are currently unable to control and treat different types of leishmaniasis and efficient vaccine and drug are not available. This study is aimed to assess the pathological aspects and drugs susceptibility of L. major which received antisense oligonucleotides (ASO) against HSP-70 and HIS-1 gene. Methods In current experimental study, we describe the anti-leishmanial effect of ASO targeting the HSP-70 and HIS-1 genes that genes play roles in stage differentiation and antileishmanial drugs susceptibility using the L. major promastigote, amastigote and cutaneous leishmaniasis (CL) in mice, respectively. Results Our results revealed that the expression of the HSP-70 and HIS-1 genes was significantly decreased in group receiving ASO. The amastigote count in macrophage in group receiving HSP-70 ASO was significantly lower than other groups. The results of in vivo experiments indicated that in the group receiving HIS-1 ASO the size of CL lesion and the susceptibility to the glucantime were significantly lower and higher than other group, respectively. Conclusion The results of current study showed that ASO as a new technique in genetic manipulation can help the control and prevent of many diseases.