Evaluation of the role of autogenous bone-marrow–derived mesenchymal stem cell transplantation for the repair of mandibular bone defects in rabbits

Saad, Khaled; Abu-Shahba, Ahmed G.; El-Drieny, Ezzat Abd-Elaziz; Khedr, Mohamed Saad

doi:10.1016/j.jcms.2015.04.013

Cited by 21 publications

(13 citation statements)

References 31 publications

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“…These bone trabeculae were limitedly increased in the 8 th week. This delaying in bone healing in control side was confirmed by other studies [30,31] .…”

Section: Discussionsupporting

confidence: 84%

In Vivo Biocompatibility of β Tri-calicum Phosphate Versus White Portland Cement in Mandibular Bone Surgical Defect in Dogs

Mohammed

Saleh

Taiema

et al. 2019

Egyptian Dental Journal

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Back ground: Synthetic bone substitutes have been advanced, and researches are developed to more effective biomaterials with promising ability to reconstruct lost bone structure. Tricalcium phosphate biomaterials and white Portland cement are among them. The aim of the current study is to assess the effect of β-tricalcium phosphate versus white Portland cement in surgically mandibular bone defect dogs. Material and methods:Six adult dogs were used. The anaesthesia was conducted by iv injection of thiopental sodium 20mg/kg.b.wt. and maintained using (atropine 0.04 mg/kg b.wt., xylazine 1mg/kg.b.wt. and diazepam 1mg/kg.b.wt.in saline iv drop by drop infusion ), three critical size defects were designed at the buccal side of the mandible. The defects were 5 cm apart from each other. One defects filled with sterile white Portland cement, the middle defect filled with β-tricalcium phosphate and the later one was left empty. The dogs were euthanized at the end of 1 and 8 week postoperatively respectively. The specimens were prepared for light microscope using H&E and Masson Tri-chrome staining. Results: The experimental sides of all periods revealed increasing the amount of new bone trabeculae extended from original bone towards the center of the defect with both white Portland cement & β-tricalcium phosphate. While, the control sides displayed few new bone trabeculae at central area and the lateral wall of the bony defects. The amount of new bone trabeculae confirmed the histological results. Conclusion: White Portland cement is a biocompatible osteoconductive economically affordable graft material and it id more efficient than β-tricalcium phosphate. (3476) Altaib Abdel Razik Mohammed, et al.

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“…These bone trabeculae were limitedly increased in the 8 th week. This delaying in bone healing in control side was confirmed by other studies [30,31] .…”

Section: Discussionsupporting

confidence: 84%

In Vivo Biocompatibility of β Tri-calicum Phosphate Versus White Portland Cement in Mandibular Bone Surgical Defect in Dogs

Mohammed

Saleh

Taiema

et al. 2019

Egyptian Dental Journal

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“…Remarkably, our approach showed no significant change when hMSCs were applied. Stem cells, which have a clearly lower cell cycle activity, are much more difficult to transfect …”

Section: Discussionmentioning

confidence: 99%

“…Stem cells, which have a clearly lower cell cycle activity, are much more difficult to transfect. 23,35,36 All trials were performed in vitro, so the influence of the extracellular matrix (ECM) on the hMSCs could not be determined. Stem cells are much more reactive in their physiological environment in the presence of an ECM and intercellular communication, which are evidently necessary for development and differentiation.…”

Section: Discussionmentioning

confidence: 99%

Functional analysis of bioactivated and antiinfective PDLLA – coated surfaces

Haidari

Boskov

Schillinger

et al. 2017

J Biomedical Materials Res

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Common scaffold surfaces such as titanium can have side effects; for example, infections, cytotoxicity, impaired osseointegration, or low regeneration rates for bone tissue. These effects lead to poor implant integration or even implant loss. Therefore, bioactive implants are promising instruments in tissue regeneration. Osteoinductive elements-such as growth factors and anti-infectives-support wound healing and bone growth and thereby enable faster osseointegration, even in elderly patients. In this study, titanium surfaces were coated with a poly-(d,l-lactide) (PDLLA) layer containing different concentrations of copolymer-protected gene vectors (COPROGs) to locally provide bone morphogenetic protein-2 (BMP-2) or activated anti-infective agents, such as chlorhexidine gluconate, triclosan, and metronidazole, to prevent peri-implantitis. The coated titanium implants were then loaded with osteoblasts, NIH 3T3 fibroblasts, and human mesenchymal stem cells in 96-well plates. When shielded by COPROGs as a protective layer and resuspended in PDLLA, BMP-2-encoding pDNA at relatively low doses (5.63 µg/implant) induced the local expression of BMP-2. A linear dose dependence, which is common for recombinant growth factors, was not found, probably due to the retention property of the PDLLA surface. PDLLA, in general, successfully retains additional elements, such as osteoconductive growth factors (BMP-2) and anti-infective agents, which was demonstrated using metronidazole, and thus prevents the systemic application of excessive doses. These bioactive implant surfaces that provide the local release of therapeutic gene vectors or anti-infective agents allow the controlled stimulation of the implant and scaffold osseointegration. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1672-1683, 2017.

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“…One study adapted a rabbit model for mandibular defects reconstruction [202]. Autogenous BMMSCs were loaded onto β-TCP micro-granules and implanted into mandibular critical-sized defects (10×15 mm) of rabbits.…”

Section: Applications Of Injectable Scaffolds In Dental and Craniomentioning

confidence: 99%

Section: Figmentioning

confidence: 99%

Injectable scaffolds: Preparation and application in dental and craniofacial regeneration

Chang

Ahuja

et al. 2017

Materials Science and Engineering: R: Reports

212

170

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Injectable scaffolds are appealing for tissue regeneration because they offer many advantages over pre-formed scaffolds. This article provides a comprehensive review of the injectable scaffolds currently being investigated for dental and craniofacial tissue regeneration. First, we provide an overview of injectable scaffolding materials, including natural, synthetic, and composite biomaterials. Next, we discuss a variety of characteristic parameters and gelation mechanisms of the injectable scaffolds. The advanced injectable scaffolding systems developed in recent years are then illustrated. Furthermore, we summarize the applications of the injectable scaffolds for the regeneration of dental and craniofacial tissues that include pulp, dentin, periodontal ligament, temporomandibular joint, and alveolar bone. Finally, our perspectives on the injectable scaffolds for dental and craniofacial tissue regeneration are offered as signposts for the future advancement of this field.

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Evaluation of the role of autogenous bone-marrow–derived mesenchymal stem cell transplantation for the repair of mandibular bone defects in rabbits

Cited by 21 publications

References 31 publications

In Vivo Biocompatibility of β Tri-calicum Phosphate Versus White Portland Cement in Mandibular Bone Surgical Defect in Dogs

In Vivo Biocompatibility of β Tri-calicum Phosphate Versus White Portland Cement in Mandibular Bone Surgical Defect in Dogs

Functional analysis of bioactivated and antiinfective PDLLA – coated surfaces

Injectable scaffolds: Preparation and application in dental and craniofacial regeneration

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