Evaluation of the Safety and Protection Efficacy of spiC and nmpC or rfaL Deletion Mutants of Salmonella Enteritidis as Live Vaccine Candidates for Poultry Non-Typhoidal Salmonellosis

Li, Qiuchun; Yue, Zhenggang; Ren, Jingwei; Qiao, Zhuang; Yin, Chao; Xian, Honghong; Yu, Yuan; Geng, Shizhong; Jiao, Xinan

doi:10.3390/vaccines7040202

Cited by 12 publications

(6 citation statements)

References 37 publications

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“…Moreover, the mutant strain was recovered in lower numbers throughout the sampling period, compared to the wild-type strain. The result is in accordance with the previous reports on the deletion of the spiC gene in the S. Pullorum or S. Enteritidis ( Li et al., 2019 ; Wang et al., 2021 ). In addition to being attenuated, the live attenuated Salmonella vaccine should have no side effects in the poultry flocks ( Yin et al., 2015 ).…”

Section: Discussionsupporting

confidence: 93%

Safety and protective efficacy of Salmonella Pullorum spiC and rfaH deletion rough mutant as a live attenuated DIVA vaccine candidate

et al. 2022

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Salmonella enterica serovar Pullorum ( S. Pullorum) causes pullorum disease ( PD ), which is an acute systemic disease, in chickens, and leads to serious economic losses in many developing countries because of its high morbidity and mortality rate in young chicks. The live-attenuated vaccine is considered to be an effective measure to control the Salmonella infection. In addition, the DIVA (differentiation of infected and vaccinated animals) feature without the interference of serological monitoring of Salmonella infection is an important consideration in the development of the Salmonella vaccine. In this study, we evaluated the immunogenicity and protective efficacy of a S. Pullorum rough mutant S06004 ΔspiCΔrfaH as a live attenuated DIVA vaccine candidate in chickens. The S06004 ΔspiCΔrfaH exhibited a significant rough lipopolysaccharides ( LPS ) phenotype which was agglutinated with the acriflavine, not with the O 9 mono antibody. Compared to the wild-type, 50% lethal dose (LD 50 ) of the rough mutant increased 100-fold confirmed its attenuation. The mutant strain also showed a decreased bacterial colonization in the spleen and liver. The immunization with the mutant strain had no effect on the body weight and no tissue lesions were observed in the liver and spleen. The high level of the S. Pullorum-specific IgG titers in the serum indicated that significant humoral immune responses were induced in the immunization group. The cellular immune responses were also elicited from the analysis of lymphocyte proliferation and expression of cytokines in the spleen. In addition, the S06004 ΔspiCΔrfaH immunized group exhibited a negative response for the serological test, while the wild-type S06004 infection group was strongly positive for the serological test showing a DIVA capability. The survival rates in the vaccinated chickens were 87% after intramuscular challenge with wild-type S. Pullorum, while the survival rates were 20% in the control groups. Overall, these results have demonstrated that the rough mutant S06004 ΔspiCΔrfaH strain can be developed as an efficient live attenuated DIVA vaccine candidate to control the systemic S. Pullorum infection without the interference of salmonellosis monitoring program in poultry.

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Section: Discussionsupporting

confidence: 93%

Safety and protective efficacy of Salmonella Pullorum spiC and rfaH deletion rough mutant as a live attenuated DIVA vaccine candidate

et al. 2022

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“…The overall eradication of S. Enteritidis in host tissues can prevent horizontal and vertical transmission of bacteria, which is a crucial index for evaluating the protective efficiency of vaccines ( Tang et al., 2019 ). Live attenuated Salmonella vaccines have been confirmed to show a strong ability to eliminate pathogens in the liver, spleen, and ileum but not in the cecum ( Li et al., 2019 ). Reported subunit vaccines such as FliC or SPI-1 effectors also had limitations in clearing Salmonella in the cecum ( Desin et al., 2011 ; Wisner et al., 2011 ; Okamura et al., 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Preclinical evaluation of OMVs as potential vaccine candidates against Salmonella enterica serovar Enteritidis infection

Jiang

Chu²,

Wang³

et al. 2022

Front. Cell. Infect. Microbiol.

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Salmonella enterica serovar Enteritidis is the most prevalent serotype that causes human infections worldwide. Consumption of S. Enteritidis-contaminated animal foods is a major source of human infections; however, eradicating bacteria from animals remains difficult. Therefore, it is necessary to develop new measures to prevent and control salmonellosis. Here, we used the outer-membrane vesicles (OMVs) of S. Enteritidis and assessed their protective efficacy and immune response in mice. Deletion of tolR in S. Enteritidis increased the production and size of OMVs compared to those in the wild type (WT) and ΔrfaQ strains. Intramuscular immunization with OMVs conferred greater protection than intraperitoneal and intranasal immunization. Moreover, OMVs extracted from both WT and ΔtolR strains provided an 83.3% protective rate in mice challenged with S. Enteritidis, which was higher than that provided by OMVs extracted from the ΔrfaQ strain. However, compared with OMVs from the ΔtolR strain, OMVs from WT and ΔrfaQ strains rapidly eradicated S. Enteritidis colonizing the liver, spleen, ileum, and cecum of BALB/c mice after immunization. Immunization with OMVs from each of the three strains induced humoral immune responses and showed no side effects on the growth of mice. Our study revealed that OMVs from various S. Enteritidis strains could be developed for use as subunit vaccine candidates against nontyphoidal Salmonella infections in mammals.

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“…Triple mutant targeting porin, carbohydrate metabolism, and type III secretion system were used by Li et al [288]. The construct combined deletion from SPI-2 (∆spiC) with OmpD porin (∆nmpC) or rfaL involved in mannitol utilization (∆rfaL) in a chicken model of S. Enteritidis infection.…”

Section: Live Pathogensmentioning

confidence: 99%

Secretory System Components as Potential Prophylactic Targets for Bacterial Pathogens

Świętnicki

2021

Biomolecules

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Bacterial secretory systems are essential for virulence in human pathogens. The systems have become a target of alternative antibacterial strategies based on small molecules and antibodies. Strategies to use components of the systems to design prophylactics have been less publicized despite vaccines being the preferred solution to dealing with bacterial infections. In the current review, strategies to design vaccines against selected pathogens are presented and connected to the biology of the system. The examples are given for Y. pestis, S. enterica, B. anthracis, S. flexneri, and other human pathogens, and discussed in terms of effectiveness and long-term protection.

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Evaluation of the Safety and Protection Efficacy of spiC and nmpC or rfaL Deletion Mutants of Salmonella Enteritidis as Live Vaccine Candidates for Poultry Non-Typhoidal Salmonellosis

Cited by 12 publications

References 37 publications

Safety and protective efficacy of Salmonella Pullorum spiC and rfaH deletion rough mutant as a live attenuated DIVA vaccine candidate

Safety and protective efficacy of Salmonella Pullorum spiC and rfaH deletion rough mutant as a live attenuated DIVA vaccine candidate

Preclinical evaluation of OMVs as potential vaccine candidates against Salmonella enterica serovar Enteritidis infection

Secretory System Components as Potential Prophylactic Targets for Bacterial Pathogens

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