Evaluation of the safety and antioxidant activities of Crocus sativus and Propolis ethanolic extracts

Ramadan, A.; Soliman, Gamal A.; Mahmoud, Sawsan S.; Nofal, Salwa M.; Abdel-Rahman, Rehab F.

doi:10.1016/j.jscs.2010.10.012

Cited by 81 publications

(60 citation statements)

References 38 publications

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“…Oral administration of PP and its main constituent was shown to be protective against acute liver damage induced by various hepatotoxicants such as carbon tetrachloride, alcohol, D-galactosamine and acetaminophen (Ramadan et al 2015;Seo et al 2003).…”

Section: R a F Tmentioning

confidence: 99%

“…The anti-inflammatory and oxyradical scavenging properties of PP attribute to its wide range of biological activities (Nakamura et al 2013). Oral administration of PP and its main constituent was shown to be protective against acute liver damage induced by various hepatotoxicants such as carbon tetrachloride, alcohol, D-galactosamine and acetaminophen (Ramadan et al 2015;Seo et al 2003).This hepato-protection may be attributed to its several pharmacological actions due to its ability to inhibit lipid peroxidation (Nakamura et al 2013). However, it is still unclear whether PP exerts protective effects against hepatic encephalopathy in rats.…”

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confidence: 99%

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Hepato- and neuro-protective influences of biopropolis on thioacetamide-induced acute hepatic encephalopathy in rats

Mostafa

Salama

Abdel-Rahman

et al. 2017

Can. J. Physiol. Pharmacol.

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Hepatic encephalopathy (HE) is a neuropsychiatric syndrome ultimately occurs as a complication of acute or chronic liver failure; accompanied by hyperammonemia. This study aimed to evaluate the potential of biopropolis as a hepato and neuroprotective agent using thioacetamide (TAA)-induced acute HE in rats as a model. Sixty Wistar rats were divided into five groups: Group 1 (normal control) received only saline and paraffin oil.Group 2 (hepatotoxic control) received TAA (300 mg/kg, once). Groups 3, 4 and 5 received TAA followed by vitamin E (100 mg/kg) and biopropolis (100 and 200 mg/kg), respectively, daily for 30 days. Evidences of hepatic encephalopathy were clearly detected in TAA-hepatotoxic group including significant elevation in the serum level of ammonia, liver functions, increased oxidative stress in liver and brain, apoptotic DNA fragmentation and overexpression of iNOS gene in brain tissue. The findings for groups administered biopropolis, highlighted its efficacy as a hepato and neuro-protectant through improving the liver functions, oxidative status and DNA fragmentation as well as suppressing the brain expression of iNOS gene. In conclusion, bioproplois, at a dose of 200 mg/kg/day protected against TAA-induced HE through its antioxidant and antiapoptotic influence; therefore, it can be used as a protective natural product.Keywords: Biopropolis; hepatic encephalopathy; thioacetamide; iNOS; vitamin E. IntroductionHepatic encephalopathy (HE) is a neuropsychiatric syndrome resulting from acute or chronic liver failure (Lizardi-Cervera et al. 2003). HE affects a huge number of patients worldwide leading to a mortality rate of 50 to 90% (Raghavan and Marik 2006). HE causes a wide range of clinical manifestations, including psychomotor dysfunctions, sensory abnormalities, poor concentration, increased reaction time and impaired memory. Patients commonly develop stupor, coma and death in severe cases.The mechanism of HE has not been fully explained. However, hyper-ammonemia has been suggested to play the main role in HE pathogenesis and to be responsible for the direct and indirect alterations in cerebral metabolism and hence encephalopathy (Frederick 2011;Mustafa et al. 2013).Thioacetamide (TAA) is used to induce an experimental rat model mimicking acute hepatic failure and HE. This model has been validated to be a satisfactory model of HE (Avraham et al. 2006). TAA undergoes extensive metabolism to highly reactive metabolites that binds to liver macromolecules resulting in hepatic necrosis, hyperammonemia (Swapna et al. 2006) and extensive oxidative stress (Sathyasaikumar et al. 2007). Studies demonstrated that centrilobular necrosis is apparent after single dose of TAA, while cirrhosis, liver cell adenomas and liver cancer usually develop after chronic administration (Waters et al. 2005).Oxidative stress has long been involved in the pathogenesis of acute and chronic liver damage in many conditions such as toxin exposures, bile duct obstruction, excess alcoholism, liver ischemia, and viral inf...

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Section: R a F Tmentioning

confidence: 99%

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confidence: 99%

Hepato- and neuro-protective influences of biopropolis on thioacetamide-induced acute hepatic encephalopathy in rats

Mostafa

Salama

Abdel-Rahman

et al. 2017

Can. J. Physiol. Pharmacol.

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“…There is no report on the phytochemistry or any other scientific study on C. mathewii. C. sativus, a sister species has been investigated for various bioactivities like antioxidant [9,10] , antityrosinase [11] , free radical scavenging and ferric reducing [12] , antibacterial [13] and inhibitory activity on amyloid-β aggregation [9] .…”

Section: Research Papermentioning

confidence: 99%

Green Synthesis and Characterization of Silver Nanoparticles from Crocus Mathewii; A Disremembered Turkish Flowering Plant

Yildiztekin¹,

Nadeem²,

Yildiztekin³

et al. 2017

pharmaceutical-sciences

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Mahmut, et al.: Characterization of Silver Nanoparticles from Crocus mathewiiThis study reports the first ever chemical study on Crocus mathewii, a Turkish endemic and forgotten flowering plant. Silver nanoparticles were green synthesized from one mmol×l -1 silver nitrate extract of C. mathewii. The water-suspended silver nanoparticles showed a peak at 423 nm in UV/Vis spectrophotometer that suggested smaller particle size due to its less surface plasmon resonance. Transmission electron microscopy analysis also proved excellent polydispersity of quasi-spherical particle size distribution with average particles of 7.5 nm (2.5-25 nm). The presence of silver in the nanoparticles was analyzed by X-ray diffraction, while Fourier transform infrared spectroscopy analysis showed the presence of various -OH and -NH containing organics. The results proved that the crude extract of C. mathewii can be used for green synthesis of silver nanoparticles in future for medicinal uses.

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“…For example, the water:methanol (50:50, v/v) extract of C. sativus have been reported to possess better antioxidant activities; even higher than that of tomatoes and carrots (Papandreou et al 2006). On the contrary, Ramadan et al (2012) has reported weak antioxidant activity of the ethanol extract of the same plant, although they concluded the extract as non-toxic. Sariri et al (2011) studied the antioxidant and antityrosinase activities of extract obtained from the methanol extract of C. sativus flowers.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant, anticholinesterase and tyrosinase inhibition activities, and fatty acids of Crocus mathewii – A forgotten endemic angiosperm of Turkey

Yildiztekin

Nadeem

Erol

et al. 2016

Pharmaceutical Biology

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Context We report the first ever chemical/biochemical study on Crocus mathewii Kerndorff (Iridaceae) -a Turkish endemic angiosperm. This plant has never been explored for its phytochemistry and bioactivities. Objective This study explores C. mathewii corm and aerial parts for the chemical and biological properties of hexane, ethyl acetate, methanol and water fractions of the extracts. Material and methods Plant material (20 g) was extracted by methanol (250 mL Â 5, 3 days each) and fractioned into hexane, ethyl acetate, methanol and water. All fractions were subjected to b-carotene-linoleic acid, DPPH Á , ABTS Á+ , CUPRAC, metal chelating and tyrosinase inhibition activities. Hexane fractions were submitted to GC-MS analysis. Results Ethyl acetate fractions showed excellent IC 50 values in DPPH Á (aerial 36.21 ± 0.76 and corm 33.87 ± 0.02 mg/L) and ABTS Á+ (aerial 33.01 ± 0.79 and bulb 27.87 ± 0.33 mg/L); higher than the IC 50 of the standard a-tocopherol (DPPH 116.25 ± 1.97; ABTS 52.64 ± 0.37 mg/L), higher than BHA in DPPH (57.31 ± 0.25 mg/L), but slightly lower in ABTS (19.86 ± 2.73 mg/L). Methanol extract of aerial parts also showed higher activity than a-tocopherol in DPPH (85.56 ± 11.51 mg/L) but slightly less (72.90 ± 3.66 mg/L) than both the standards in ABTS. Linoleic (aerial 53.9%, corm 43.9%) and palmitic (aerial 22.2%, corm 18%) were found as the major fatty acids. Discussion and conclusion Some fractions of C. mathewii showed higher antioxidant activities than the standards. There is a need to explore more about this plant. ARTICLE HISTORY

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Evaluation of the safety and antioxidant activities of Crocus sativus and Propolis ethanolic extracts

Cited by 81 publications

References 38 publications

Hepato- and neuro-protective influences of biopropolis on thioacetamide-induced acute hepatic encephalopathy in rats

Hepato- and neuro-protective influences of biopropolis on thioacetamide-induced acute hepatic encephalopathy in rats

Green Synthesis and Characterization of Silver Nanoparticles from Crocus Mathewii; A Disremembered Turkish Flowering Plant

Antioxidant, anticholinesterase and tyrosinase inhibition activities, and fatty acids of Crocus mathewii – A forgotten endemic angiosperm of Turkey

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