Evaluation of the safety, immunogenicity and efficacy of a new live-attenuated lumpy skin disease vaccine in India

Kumar, Naveen; Barua, Sanjay; Kumar, Ram; Khandelwal, Nitin; Kumar, Amit; Verma, Ashu; Singh, Lokender; Godara, Bhagraj; Chander, Yogesh; Kumar, Garvit; Riyesh, Thachamvally; Sharma, Deepak Kumar; Pathak, Archana; Kumar, Sanjay; Dedar, R. K.; Mehta, Vishal; Gaur, Mitesh; Bhardwaj, Bhupendra; Vyas, Vithilesh; Chaudhary, Sarjeet; Yadav, Vijaypal; Bhati, Adrish; Kaul, Rakesh; Bashir, Arif; Andrabi, Anjum; Yousuf, Raja Wasim; Koul, Abhimanyu; Kachhawaha, Subhash; Gurav, Amol; Gautam, Siddharth; Tiwari, Hari Audh; Munjal, Vijay Kumar; Gupta, M. K.; Kumar, Rajender; Gulati, Baldev R.; Misri, Jyoti; Kumar, Ashok; Mohanty, Ashok Kumar; Nandi, Sukdeb; Pal, Yash; Dutt, Triveni; Tripathi, Bhupendra Nath

doi:10.1080/21505594.2023.2190647

Cited by 29 publications

(33 citation statements)

References 58 publications

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“…This is also supported by Kumar who also observed that animals with highest antibody titres did not show signi cant cellular responses and vice-versa. CMI responses evaluated by DTH reaction following virus inoculation and quanti cation of IFN-gamma with evidence to suggest the role of CD4 + and CD8 + cells should be taken up to obtain conclusive results on the e cacy of a vaccine [12].…”

Section: Discussionmentioning

confidence: 99%

Emergency vaccination of cattle with Goatpox vaccine against LSD in India: Evaluation of efficacy and potency

Balam,

et al. 2024

Preprint

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Lumpy skin disease (LSD) is a re-emerging transboundary viral disease of cattle and buffaloes with severe economic impact and is listed as a notifiable disease by the World Organization for Animal Health. Mass vaccination of susceptible animals is the foremost approach in tackling this infectious disease. Although the efficacy and immunogenicity of homologous LSD vaccine (Lumpi-ProVacInd) is known to be excellent, cost of vaccine production along with the neethling responses observed in the vaccinated animals and limited availability for the field use during the current outbreak are the major limitations. Live attenuated Goatpox vaccine of Uttarkashi strain is authorized by the government for control of LSD as an emergency measure in India during 2022. The present study deals with an objective to determine the optimum dose of Goatpox vaccine against LSD infection in cattle. Vaccination trial was conducted in randomly selected heifers placed into four groups (A, B, C and D) of eight animals each. Group A served as control group, while groups B, C and D were vaccinated with 1mL, 2mL and 3mL of 1 X 103.0 TCID50/dose of Goatpox vaccine respectively. Group D vaccinated with 3 times the dose used in goats produced the best humoral immunity and the responses persisted till the end of the trail i.e., 35 days post vaccination (p > 0.05).

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Section: Discussionmentioning

confidence: 99%

Emergency vaccination of cattle with Goatpox vaccine against LSD in India: Evaluation of efficacy and potency

Balam,

et al. 2024

Preprint

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“…A field study was conducted with the inactivated LSD vaccine and showed that 70% of the animals developed neutralizing antibodies when VNT was used [ 62 ]. Other studies have reported seroconversions of 85.15% of animals vaccinated with LSD live attenuated vaccine on day 30 pv [ 95 ]. Regarding CBPP, there is a need to have tests that can provide sensitive and specific outcomes in the field [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a Combined Live Attenuated Vaccine against Lumpy Skin Disease, Contagious Bovine Pleuropneumonia and Rift Valley Fever

Bamouh,

Elarkam,

Elmejdoub

et al. 2024

Vaccines

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The use of effective vaccines is among the most important strategies for the prevention and progressive control of transboundary infectious animal diseases. However, the use of vaccine is often impeded by the cost, a lack of cold chains and other factors. In resource-limited countries in Africa, one approach to improve coverage and reduce cost is to vaccinate against multiple diseases using combined vaccines. Therefore, the objective of this study was to evaluate a combined vaccine for the prevention and control of Lumpy Skin Disease (LSD), Contagious Bovine Pleuropneumonia (CBPP) and Rift Valley fever (RVF). The LSD and CBPP were formulated as a combined vaccine, and the RVF was formulated separately as live attenuated vaccines. These consisted of a Mycoplasma MmmSC T1/44 strain that was propagated in Hayflick-modified medium, RVF virus vaccine, C13T strain prepared in African green monkey cells (Vero), and the LSDV Neethling vaccine strain prepared in primary testis cells. The vaccines were tested for safety via the subcutaneous route in both young calves and pregnant heifers with no side effect, abortion or teratogenicity. The vaccination of calves induced seroconversions for all three vaccines starting from day 7 post-vaccination (PV), with rates of 50% for LSD, 70% for CBPP and 100% for RVF, or rates similar to those obtained with monovalent vaccines. The challenge of cattle vaccinated with the LSD/CBPP and the RVF vaccine afforded full protection against virulent strains of LSDV and RVFV. A satisfactory level of protection against a CBPP challenge was observed, with 50% of protection at 6 months and 81% at 13 months PV. A mass vaccination trial was performed in four regions of Burkina Faso that confirmed safety and specific antibody responses induced by the vaccines. The multivalent LSD/CBPP+RVF vaccine provides a novel and beneficial approach to the control of the three diseases through one intervention and, therefore, reduces the cost and improves vaccination coverage.

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“…While smallpox has been eradicated, the monkeypox virus (MPXV) has recently become an international concern for human health 1 . Likewise, the lumpy skin disease virus (LSDV) has emerged as an important pathogen in cattle population across many Asian countries with regard to animal health 2–7 . The poxviruses are usually host‐specific 8 .…”

Section: Introductionmentioning

confidence: 99%

Hesperetin blocks poxvirus replication with a low tendency to select for drug‐resistant viral variants

Verma,

Dedar,

Kumar

et al. 2024

Journal of Medical Virology

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In this study, we demonstrated the antiviral efficacy of hesperetin against multiple poxviruses, including buffalopox virus (BPXV), vaccinia virus (VACV), and lumpy skin disease virus (LSDV). The time‐of‐addition and virus step‐specific assays indicated that hesperetin reduces the levels of viral DNA, mRNA, and proteins in the target cells. Further, by immunoprecipitation (IP) of the viral RNA from BPXV‐infected Vero cells and a cell‐free RNA‐IP assay, we demonstrated that hesperetin‐induced reduction in BPXV protein synthesis is also consistent with diminished interaction between eukaryotic translation initiation factor eIF4E and the 5′ cap of viral mRNA. Molecular docking and MD simulation studies were also consistent with the binding of hesperetin to the cap‐binding pocket of eIF4E, adopting a conformation similar to m7GTP binding. Furthermore, in a BPXV egg infection model, hesperetin was shown to suppress the development of pock lesions on the chorioallantoic membrane and associated mortality in the chicken embryos. Most importantly, long‐term culture of BPXV in the presence of hesperetin did not induce the generation of drug‐resistant viral mutants. In conclusion, we, for the first time, demonstrated the antiviral activity of hesperetin against multiple poxviruses, besides providing some insights into its potential mechanisms of action.

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Evaluation of the safety, immunogenicity and efficacy of a new live-attenuated lumpy skin disease vaccine in India

Cited by 29 publications

References 58 publications

Emergency vaccination of cattle with Goatpox vaccine against LSD in India: Evaluation of efficacy and potency

Emergency vaccination of cattle with Goatpox vaccine against LSD in India: Evaluation of efficacy and potency

Evaluation of a Combined Live Attenuated Vaccine against Lumpy Skin Disease, Contagious Bovine Pleuropneumonia and Rift Valley Fever

Hesperetin blocks poxvirus replication with a low tendency to select for drug‐resistant viral variants

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