Evaluation of the schistosomicidal, antioxidant and anti-inflammatory activities of the ethyl acetate fraction from Ozoroa pulcherrima Schweinf. Roots on Schistosoma mansoni-induced liver pathology in mice and its phytochemical characterization

Jatsa, Hermine Boukeng; Feussom, Nestor Gipwe; Femoe, Ulrich Membe; Kenfack, Mérimé Christian; Nkondo, Emilienne Tienga; Fassi, Joseph Bertin Kadji; Simo, Nadège Distele; Moaboulou, Cyriaque; Dongmo, Calvine Noumedem; Tsague, Christelle Dongmo; Dongo, Étienne; Kamtchouing, Pièrre; Tchuenté, Louis-Albert Tchuem

doi:10.1016/j.jep.2019.111883

Cited by 7 publications

(23 citation statements)

References 47 publications

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“…Our previous studies have identified the presence of reducing sugar, anthraquinones, terpenoids, tannins, phenols, flavonoids alkaloids, cardiac glycosides, triterpenes and lipid steroid in methanolic extract of O. pulcherrima [19]. Additionally, various terpenoids, flavonoids and new alkaloids have been isolated and characterized in its ethyl acetate fraction [38].…”

Section: Discussionmentioning

confidence: 99%

In vitro Activity of Ozoroa pulcherrima Schweinf. Extracts and Fractions on Schistosoma mansoni Cercariae and Adult Worms

Feussom

Jatsa

Kenfack

et al. 2020

EJMP

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Aims: Continuous attempts are being made to develop new and more effective drugs for the treatment of schistosomiasis. Ozoroa pulcherrima Schweinf. is a medicinal plant used in Africa for the treatment of dysmenorrhea, lower abdominal pain, dystocia and intestinal helminthiasis. This study provides findings on the cercaricidal and schistosomicidal activity of extracts and fractions of Ozoroa pulcherrima in in vitro assays. Methodology: The aqueous and methanolic extracts from Ozoroa pulcherrima root parts (62.5 – 2000 µg/mL), as well as the methanol derived fractions (n-hexane and ethyl acetate: 31.25 – 1000 µg/mL) were tested on cercariae and adult worms of Schistosoma mansoni. Niclosamide-olamine 5% (1 µg/mL) and praziquantel (10 µg/mL) were respectively used as reference drugs. During the assays, the mortality of cercariae after 2 hours, and adult worms’ mobility and mortality after 48 hours of incubation were evaluated. Results: Ozoroa pulcherrima extracts and fractions significantly increased cercariae and worm mortality in a concentration-dependent manner. The methanolic extract was the most active on cercariae with a LC50of 20.65 µg/mL after 30 minutes, while the n-hexane fraction was the most active on worm with a LC50 of 79.54 μg/mL (65.58 – 96.47 μg/mL) after 48 hours. Significant reduction of motor activity (18.47 to 100%) was recorded for surviving worms incubated in different concentrations of the extracts and fractions. Conclusion: This study proves that Ozoroa pulcherrima extracts and fractions have cercaricidal and schistosomicidal activities. Ozoroa pulcherrima may have great potential as an anti-schistosomal agent for further research.

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Section: Discussionmentioning

confidence: 99%

In vitro Activity of Ozoroa pulcherrima Schweinf. Extracts and Fractions on Schistosoma mansoni Cercariae and Adult Worms

Feussom

Jatsa

Kenfack

et al. 2020

EJMP

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“…Therefore, the host will immediately use antioxidants to counteract the harmful action of ROS overload. The consequence will be the reduction of hepatic SOD, CAT, and GSH levels [4,30,45,[64][65][66]80,81]. Administration of PZQ to S. mansoni-infected mice fed with a standard or a protein-deficient diet resulted in a reduction of the lipid peroxidation (MDA) and improved enzymatic and non-enzymatic antioxidants levels (SOD, CAT, and GSH).…”

Section: Discussionmentioning

confidence: 99%

“…Because S. mansoni worms are unable to synthesize cholesterol required for their growth and egg production, they absorb it from the host’s bloodstream, hence dropping the levels of total cholesterol, cholesterol LDL, and triglycerides [76]. Hepatocellular injury leading to the modification of transaminases (ALT and AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), and total bilirubin (BIL) levels is also common in S. mansoni infection [30,45,64,66]. The presence of S. mansoni eggs in the liver parenchyma initiates an inflammatory process that gives rise to the formation of granulomas.…”

Section: Discussionmentioning

confidence: 99%

Protein undernutrition reduces the efficacy of praziquantel in a murine model ofSchistosoma mansoniinfection

Fassi

Jatsa

Femoe

et al. 2022

Preprint

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Background: Undernutrition and schistosomiasis are public health problems and often occur in low and middle-income countries. Protein undernutrition can alter the host-parasite environment system and aggravate the course of schistosomiasis. This study aimed to assess the impact of a protein-deficient diet on the efficacy of praziquantel. Methodology/principal findings: Thirty-day-old mice were fed with a protein-deficient diet, and 40 days later, they were individually infected with fifty Schistosoma mansoni cercariae. A 28-day-treatment with praziquantel at 100 mg/kg for five consecutive days followed by distilled water begins at the 36th day post-infection. Mice were sacrificed on the 64th day post-infection. We determined the parasitological burden, liver and intestine histomorphometry, liver injury, and immunomodulation parameters. Praziquantel treatment of infected mice fed with a standard diet (IN-PZQ) resulted in a significant reduction of worm and egg burdens and a normalization of iron and calcium levels. The therapy also improved schistosomiasis-induced hepatopathy and oxidative stress. The anti-inflammatory and immunomodulatory activities of praziquantel were also significant in these mice. When infected mice receiving the protein-deficient diet were treated with praziquantel (ILP-PZQ), the body weight loss and hepatomegaly were not alleviated, and the worm and liver egg burdens were significantly higher than those of IN-PZQ mice (P < 0.001). The treatment did not reduce the increased activities of ALT and γ-GGT, the high malondialdehyde concentration, and the liver granuloma volume. The iron and calcium levels were not ameliorated and differed from those of IN-PZQ mice (P < 0.001 and P < 0.05). Moreover, in these mice, praziquantel treatment did not reverse the high level of IL-5 and the low mRNA expression of CCL3/MIP-1α and CXCL-10/IP-10 induced by S. mansoni infection. Conclusion/significance: These results demonstrated that a protein-deficient diet reduced the schistosomicidal, antioxidant, anti-inflammatory, and immunomodulatory activities of praziquantel.

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“…The phytochemical analysis of Ozoroa pulcherrima showed the presence of compounds such as phenols, flavonoids, terpenes, condensed and hydrolyzable tannins, triterpenes and saponins. (JATSA et al, 2019). Ozoroa paniculosa and Ozoroa Mucronata presented phenolic compounds, proanthocyanidins, galotanins and flavonoids (AHMED et al, 2014).…”

Section: Extract Yield and Phytochemical Screeningmentioning

confidence: 99%

“…The chemical composition of other species of Ozoroa corroborate with some classes of compounds identified in O. obovata. From the roots of O. pulcherrima, a derivative of phenolic acid has been identified (JATSA et al, 2019) and two anacardic acids have already been isolated (CHRISTELLE et al, 2011). A flavonoid and several anacardic acids were isolated from the roots of O. insignis ABREU, 2006, NG'ANG'A et al, 2009.…”

Section: Identification Of the Compounds Present In The Ethanolic Extmentioning

confidence: 99%

Chemical profile of the twigs of Ozoroa obovata by HPLC-MS-ESI and antimicrobial activity

João

Silva

Prado

et al. 2021

Rev. Bras. de Cien., Tec. e Inov.

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In Mozambique, a large part of the population depends on plants for the treatment of various diseases. However, some of them have been little studied in relation to chemical and biological aspects. Among these species, Ozoroa obovata is widely used in traditional medicine in Mozambique. The factors that influence the use of medicinal plants in developing countries are mainly cultural habits, the inefficiency of the health system and the high cost of medicines. This work aimed to study the chemical composition and evaluate the antimicrobial activity of the twigs of O. obovata. Through the analysis by liquid chromatography coupled to mass spectrometry with electrospray ionization (HPLC-MS-ESI), it was possible to propose that in the ethanolic extract of the twigs, most of the constituents belong to the classes of phenolic acids and flavonoid. The phenolic compounds were represented by quinic, gallic and protocatechuic acids and five anacardic acids, whereas flavonoids were represented by mangiferin, taxifolin and quercetin mono and dihexoside. The extract was evaluated against bacteria in the oral cavity and showed moderate activity against Streptococcus mutans, S. mitis and Porphyromonas gingivalis (minimum inhibitory concentration-MIC 400 µg mL-1). In relation antifungal test, the extract showed activity against Candida albicans with MIC of 3000 µg mL-1. The biological results indicated that the twigs of O. obovata have bioactive metabolites with antimicrobial potential.

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Evaluation of the schistosomicidal, antioxidant and anti-inflammatory activities of the ethyl acetate fraction from Ozoroa pulcherrima Schweinf. Roots on Schistosoma mansoni-induced liver pathology in mice and its phytochemical characterization

Cited by 7 publications

References 47 publications

In vitro Activity of Ozoroa pulcherrima Schweinf. Extracts and Fractions on Schistosoma mansoni Cercariae and Adult Worms

In vitro Activity of Ozoroa pulcherrima Schweinf. Extracts and Fractions on Schistosoma mansoni Cercariae and Adult Worms

Protein undernutrition reduces the efficacy of praziquantel in a murine model ofSchistosoma mansoniinfection

Chemical profile of the twigs of Ozoroa obovata by HPLC-MS-ESI and antimicrobial activity

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