Evaluation of the Seasonality and Extraction Method on the Polar Extracts of Croton grewioides Baill. by Chromatogram Fingerprinting and Isolation of a New Triglycosylated Flavonoid

Prado, Vilma M. J.; Jesus, Raphael Amancio de; Oliveira, Julio Manoel Andrade; Pereira, Camila Santos Almeida; Blank, Arie Fitzgerald; Pereira-Filho, Edenir Rodrigues; Cass, Quézia B.; Lima, Juliana M.; Ferreira, Antônio G.; Nogueira, Paulo C.L.; Moraes, Valéria R. S.

doi:10.21577/0103-5053.20200190

Cited by 2 publications

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“…Plant material -Leaves of four accessions of C. grewioides belong to the Active Germplasm Bank of the Federal University of Sergipe. Voucher herbarium specimens of each accession were deposited under numbers 28256, 25138, 28251, 23259, named as 101, 107, 113 and 126, respectively, at the Federal University of Sergipe Herbarium, Department of Biology (SISGEN register number A8CCB3B), as described by Prado et al (2021) (Supplementary Material).…”

Section: Methodsmentioning

confidence: 99%

“…Sample preparation -Aqueous extracts were prepared by the decoction as described by Prado et al (2021) (Supplementary Material), and infusion methods. For infusion, 2 g of leaves was poured into 200 mL of ultrapure water at 92 °C (heated in a microwave oven) and allowed to rest for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…The species C. grewioides Baill., belonging to the genus Croton, the second largest genus of the Euphorbiaceae family, with more than 1300 registered species, is endemic in the northeast of Brazil, being found in several Brazilian states, and is distributed throughout the caatinga biome. Known locally as "canelinha-de-cheiro," the tea made from the leaves and branches of this species has been popularly used for the treatment of influenza, coughs, fevers, and headache (Silva et al 2016a;Prado et al 2021). Most of the reported studies concerning this species have investigated the essential oil, which has shown characteristic activities including pupicidal, larvicidal, insecticidal, and bactericidal (Silva et al 2016a;Medeiros et al 2017;Castro el al.…”

Section: Introductionmentioning

confidence: 99%

“…2019). So far, the literature only reports two studies concerning phytochemical investigation of extracts of C. grewioides, one describing the isolation of derivatives of diterpenes, phenols, and steroids, and the other the isolation of aglycone and glycoside flavonoids (Medeiros 2012;Prado et al 2021).…”

Section: Introductionmentioning

confidence: 99%

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Biological studies and chromatograms aided by chemometric analysis in evaluation of seasonality and extraction method of Croton grewioides extracts

et al. 2022

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The genus Croton is the second largest genus of Euphorbiaceae family, comprising more than 1300 registered species. The species Croton grewioides is endemic in the northeast of Brazil and distributed mainly throughout the caatinga biome presenting, in its essential oils, various compounds with biological activities such as pupicidal, larvicidal, insecticidal, and bactericidal. The secondary metabolites commonly found in their extracts include diterpenes, phenols, steroids, and aglycone and glycoside flavonoids derivatives, which can undergo variations based on climatic changes that modify processes such as photosynthesis, transpiration and nutrient cycle. To understand the effects of seasonal variability and extraction method on the biological activities of polar extracts from C. grewioides, fingerprint chromatograms were analyzed aided by chemometric analysis. In vitro assays of cytotoxicity against HL-60 and HepG2 cancer cells and anti-acetylcholinesterase activity were performed. Seasonal effects could only be observed in the chemical profiles of the methanolic and ethanolic extracts from accessions 113 and 126, respectively. Considering the extraction method, the chromatograms of aqueous and hydroalcoholic extracts of all the accessions were similar, but differed from those for the corresponding methanolic and ethanolic extracts. Five hexane partitions were active against HL-60 cell line, displaying inhibition values between 81 and 93%. Only the ethanolic extract of accession 101 (winter), the methanolic partition from the methanolic extract, and the decoction of accession 113 (winter) presented high inhibition values against the acetylcholinesterase enzyme. This study enabled elucidation of the effects of seasonality and extraction method on the biological activities presented by the C. grewioides extracts.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biological studies and chromatograms aided by chemometric analysis in evaluation of seasonality and extraction method of Croton grewioides extracts

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“…The aqueous extracts prepared by decoction were obtained following the methodology described by Prado et al, 34 with some modifications.…”

Section: Decoction Extractionmentioning

confidence: 99%

Microwave and ultrasound extraction of antioxidant phenolic compounds from Lantana camara Linn. leaves: Optimization, comparative study, and FT‐Orbitrap MS analysis

de Jesus,

da Silva,

Wisniewski

et al. 2024

Phytochemical Analysis

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IntroductionThe species Lantana camara is used in folk medicine. The biological activities of this medicinal plant are attributable to the presence of various derivatives of triterpenoids and phenolic compounds present in its preparations, indicating excellent economic potential.ObjectiveIn this study, the operational conditions of ultrasound‐assisted extraction (UAE) and microwave‐assisted extraction (MAE) were optimized using Box–Behnken design to improve the total phenolic content (TPC) recovered in hydroethanolic extracts of L. camara leaves.Material and methodsThe TPC, total flavonoid content (TFC), and antioxidant activities of the hydroalcoholic extracts of L. camara, prepared by UAE and MAE under the optimized extraction conditions, were compared with those of the extracts obtained by conventional extraction methods.ResultsUnder the optimal conditions, the extracts obtained by UAE (35% ethanol, 25 min, and a solvent‐to‐solid ratio of 60:1 mL/g) and by MAE (53% ethanol, 15 min, and 300 W) provided high yields of 32.50% and 38.61% and TPC values of 102.89 and 109.83 mg GAE/g DW, respectively. The MAE extract showed the best results with respect to TPC, TFC, and antioxidant activities, followed by extracts obtained by UAE, Soxhlet extraction, decoction, maceration, and infusion, in that order.ConclusionThe results obtained indicate that L. camara may be used as an important source of antioxidant phenolic compounds to obtain products with high biological and economic potential, especially when the extraction process is performed under appropriate conditions using MAE and/or UAE, employing environmentally friendly solvents such as water and ethanol.

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Evaluation of the Seasonality and Extraction Method on the Polar Extracts of Croton grewioides Baill. by Chromatogram Fingerprinting and Isolation of a New Triglycosylated Flavonoid

Cited by 2 publications

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Biological studies and chromatograms aided by chemometric analysis in evaluation of seasonality and extraction method of Croton grewioides extracts

Biological studies and chromatograms aided by chemometric analysis in evaluation of seasonality and extraction method of Croton grewioides extracts

Microwave and ultrasound extraction of antioxidant phenolic compounds from Lantana camara Linn. leaves: Optimization, comparative study, and FT‐Orbitrap MS analysis

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