Evaluation of the short‐term host response and biomechanics of an absorbable poly‐4‐hydroxybutyrate scaffold in a sheep model following vaginal implantation

Diedrich, Chantal M.; Guler, Zeliha; Hympánová, Lucie; Vodegel, Eva V.; Zündel, Manuel; Mazza, Edoardo; Deprest, Jan; Roovers, Jan Paul

doi:10.1111/1471-0528.17040

Cited by 20 publications

(45 citation statements)

References 38 publications

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Section: Role Of Fibroblasts In Wound Healing and Tissue Regenerationmentioning

confidence: 99%

“…Degradable materials can provide an advantage in terms of reducing clinical complications in the long term [ 28 ]. A gradual implant degradation induces functional vaginal tissue with mechanical integrity and results in a moderate or decreased inflammatory response over time [ 28 , 83 ]. Poly(lactic acid) (PLA) dramatically promoted the formation of ECM by increasing the attachment and metabolic activity of fibroblasts on PLA meshes as compared to other meshes: AlloDerm (LifeCell Corp., Branchburg, NJ, USA), cadaveric dermis, porcine dermis, polypropylene, sheep forestomach, and porcine small intestinal submucosa (SIS) [ 84 ].…”

Section: Role Of Fibroblasts In Wound Healing and Tissue Regenerationmentioning

confidence: 99%

“…Poly(l-lactide-co-caprolactone) (PLCL) and fibrinogen electro-spun scaffolds promote initial angiogenesis and tissue remodeling at the graft site, and significantly improve the symptoms of patients with prolapse [ 87 ]. Our recent in vivo study [ 28 ], where we investigated host response to P4HB implants, showed a lower inflammatory response and higher tissue remodeling relative to PP implants 6 months after vaginal implantation into sheep. In addition, we observed milder myofibroblast differentiation in P4HB at 6 months as compared to PP.…”

Section: Role Of Fibroblasts In Wound Healing and Tissue Regenerationmentioning

confidence: 99%

“…The stiffness of the implant strongly correlates with implant weight and porosity [ 14 ]. Constant applied load after implantation can cause an increase in the implant stiffness, collapse of the pores, and therefore a reduction of porosity, which in turn may lead to vaginal degeneration and eventually exposure [ 28 , 88 , 89 ]. Therefore, studies on tissue-engineered implants where a cyclic loading is applied to mimic the pelvic floor are performed.…”

Section: Role Of Fibroblasts In Wound Healing and Tissue Regenerationmentioning

confidence: 99%

“…Either in NTR or POP surgery with an implant, wound healing and tissue regeneration are highly needed for a successful surgical outcome, which is functional and mechanically self-sufficient pelvic tissue. This can mainly be achieved by a mild and well-orchestrated host response by triggering collagen metabolism without creating fibrotic tissue [ 28 ]. The formation of a healthy, functional, new connective tissue will not only improve the surgical outcome, but also contribute to a better tissue repair with less complications.…”

Section: Introductionmentioning

confidence: 99%

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Role of Fibroblasts and Myofibroblasts on the Pathogenesis and Treatment of Pelvic Organ Prolapse

Guler

Roovers

2022

Biomolecules

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Pelvic organ prolapse (POP) is a multifactorial connective tissue disorder caused by damage to the supportive structures of the pelvic floor, leading to the descent of pelvic organs in the vagina. In women with POP, fibroblast function is disturbed or altered, which causes impaired collagen metabolism that affects the mechanical properties of the tissue. Ideal surgical repair, either native tissue repair or POP surgery using an implant, aims to create a functional pelvic floor that is load-bearing, activating fibroblasts to regulate collagen metabolism without creating fibrotic tissue. Fibroblast function plays a crucial role in the pathophysiology of POP by directly affecting the connective tissue quality. On the other hand, fibroblasts determine the success of the POP treatment, as the fibroblast-to-(myo)fibroblast transition is the key event during wound healing and tissue repair. In this review, we aim to resolve the question of “cause and result” for the fibroblasts in the development and treatment of POP. This review may contribute to preventing the development and progress of anatomical abnormalities involved in POP and to optimizing surgical outcomes.

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Section: Role Of Fibroblasts In Wound Healing and Tissue Regenerationmentioning

confidence: 99%

Section: Role Of Fibroblasts In Wound Healing and Tissue Regenerationmentioning

confidence: 99%

Section: Role Of Fibroblasts In Wound Healing and Tissue Regenerationmentioning

confidence: 99%

Section: Role Of Fibroblasts In Wound Healing and Tissue Regenerationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Role of Fibroblasts and Myofibroblasts on the Pathogenesis and Treatment of Pelvic Organ Prolapse

Guler

Roovers

2022

Biomolecules

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Impact of sterilization technique on electrospun poly(4‐hydroxybutyrate) membranes for biomedical application

Chen,

Yuan,

et al. 2024

J of Applied Polymer Sci

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Bioabsorbable poly(4‐hydroxybutyrate) (P4HB) electrospun membrane has been widely used in various biomedical applications due to its biocompatibility and unique mechanical properties. When commercialization is envisaged, a sterilization procedure aiming to prevent bacterial contamination of biomedical devices is indispensable. Herein, P4HB membranes were sterilized by using different sterilization techniques. It's observed that ethylene oxide (EO), ultraviolet radiation (UV), and electron beam (E‐beam) irradiation do not influence the morphology of fiber membranes. Analysis of the data revealed that E‐beam irradiation sterilization can induce chain scission of P4HB. The EO sterilization process can increase the melting point of P4HB electrospun membranes. None of the sterilization methods showed a significant effect on the hemocompatibility of the P4HB membranes. Furthermore, the cytocompatibility experiment indicated that none of the P4HB membranes had any toxic effects on L929 cells. Taken together, selecting a suitable sterilization method that effectively sterilizes and does less damage during the sterilization process is critical to P4HB membranes for biomedical application.

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Vaginal changes after ovariectomy in ewes: A large animal model for genitourinary syndrome of menopause

Vodegel

Güler

Ras

et al. 2023

Intl J Gynecology & Obste

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ObjectiveTo evaluate the effect of iatrogenic menopause on the physiology of the vagina of the ewe and to evaluate if vaginal changes in ewes can be translated to women with genitourinary syndrome of menopause (GSM).MethodsPreclinical research with Dohne Merino ewes. Iatrogenic menopause was induced by bilateral ovariectomy (OVX). Animals were randomized for surgery, blinded for allocation and outcome assessment. Differences between groups were determined by linear regression analyses at 5 months after OVX. Outcome measures were vaginal epithelial thickness, pH, vaginal maturation value, vaginal maturation index, epithelial glycogen accumulation, content of elastin fibers, collagen, and vascularity.ResultsOVX ewes (n = 20) showed epithelial thinning of the vaginal wall from 146 μm to 47 μm (mean, P < 0.001). Furthermore, epithelial glycogen accumulation and vascularity of the vaginal wall significantly decreased (43% and 23%, respectively) as compared with the control group (no intervention; n = 5). No significant differences were found for other outcome measures.ConclusionThis study established the ewe as a suitable large animal model for GSM. Furthermore, the similar relevant outcomes in humans and ewes hold great value for future translational research for the evaluation and optimization of different treatment modalities for GSM.

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Evaluation of the short‐term host response and biomechanics of an absorbable poly‐4‐hydroxybutyrate scaffold in a sheep model following vaginal implantation

Abstract: Objective: To evaluate the host-and biomechanical response to a fully absorbable poly-4-hydroxybutyrate (P4HB) scaffold in comparison with the response to polypropylene (PP) mesh. Design:In vivo animal experiment.

Cited by 20 publications

References 38 publications

Role of Fibroblasts and Myofibroblasts on the Pathogenesis and Treatment of Pelvic Organ Prolapse

Role of Fibroblasts and Myofibroblasts on the Pathogenesis and Treatment of Pelvic Organ Prolapse

Impact of sterilization technique on electrospun poly(4‐hydroxybutyrate) membranes for biomedical application

Vaginal changes after ovariectomy in ewes: A large animal model for genitourinary syndrome of menopause

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