Evaluation of the SsIR/NIE recombinant antigen ELISA for the follow up of patients infected by <i>Strongyloides stercoralis</i>: a diagnostic study

Prato, Marco; Tamarozzi, Francesca; Tais, Stefano; Rizzi, Eleonora; Mazzi, Cristina; Buonfrate, Dora

doi:10.1017/s0031182024000027

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…Recently, the WHO categorized S. stercoralis as an STH, particularly regarding preventive chemotherapy, and encourages the development of more sensitive and specific rapid diagnostic tests, as well as biomarkers and procedures for disease control [10]. In this regard, previous studies have suggested that the use of serological techniques may solve the problem of having to use additional and specific parasitological methods for S. stercoralis for diagnosis and follow-up [5,11].…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Intestinal Parasites, Protozoans and Soil-Transmitted Helminths, in Children from Communities of Northern Argentina after the Interruption of Deworming

Candela,

Cimino,

Sandon

et al. 2024

Parasitologia

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Soil-transmitted helminths (STHs) are a group of parasites that are globally distributed and are the most prevalent neglected disease (NTD) in Latin America and the Caribbean (LAC); their presence is associated with health and development problems. In Argentina, their distribution is heterogenous, and there are highly endemic areas in the north of the country. The World Health Organization (WHO) recommends the mass deworming of children as a first-line strategy for the prevention and control of STHs and recently also encourage the development of more sensitive diagnostic tests. The aim of this study was to determine the prevalence of STHs in Tartagal (Salta, Argentina) after four years of deworming interruption with albendazole and ivermectin. A total of 437 fecal samples were analyzed using standard coprological techniques, a subset of which were selected to molecularly typify protozoan parasites; 257 blood samples were analyzed for the presence of specific antibodies to the STH Strongyloides stercoralis. The most prevalent protozoan species were G. intestinalis (19.6–49.2%) and B. hominis (19.1–38.5%). Molecular characterization allowed us to evidence possible zoonotic or human-to-human transmission pathways for Giardia intestinalis or Blastocystis spp., while serology for S. stercoralis proved to be a useful screening tool for monitoring this parasite after treatment. In general, a decrease in the prevalence of STHs was observed in the area, from 60% to 2.9–20% for hookworms and from 51% to 1–9.3% for S. stercoralis four years after treatment, demonstrating the effectiveness and duration of anthelmintic treatment with these two drugs.

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Section: Introductionmentioning

confidence: 99%

Prevalence of Intestinal Parasites, Protozoans and Soil-Transmitted Helminths, in Children from Communities of Northern Argentina after the Interruption of Deworming

Candela,

Cimino,

Sandon

et al. 2024

Parasitologia

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Immunodominant Molecules for the Immunodiagnosis of Strongyloides stercoralis Infection

Oliveira,

de Souza,

Barreto

et al. 2025

Diagnostic Microbiology and Infectious Disease

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Technical evaluation of the InBios Strongy Detect IgG ELISA assay for the diagnosis of Strongyloides stercoralis infection

Roose,

Prato,

Kazienga

et al. 2024

Parasites Vectors

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Background Strongyloidiasis is a neglected tropical disease (NTD) caused by the soil-transmitted helminth Strongyloides stercoralis, recently included in the 2030 targets of the World Health Organization for the control of STHs. Assessment of infection prevalence is fundamental for decision-making about the implementation of control programs, but diagnostic assays to be applied in such context require evaluation. Seroassays based on recombinant antigens, which could be produced in a standardized and scalable manner, are particularly appealing for use in control programs. In this study, we performed a technical evaluation of the InBios Strongy Detect IgG ELISA, based on recombinant antigens NIE and SsIR, which has shown promising for field use. Methods A total of 46 plasma samples from Ethiopian children were used for this technical evaluation. Repeatability was evaluated on duplicate samples per plate, on four plates per day for 3 days using Bland–Altman plots, analysis of residuals, and variance components analysis. Three samples were selected for evaluation of the uniformity of test results within a single plate (border effect) by two-sided t-test. Correlation between samples and internal ELISA positive controls was analyzed using Spearman’s rank correlation coefficient applied on the results of 777 samples analyzed with the assay in a previous field-based study. Results Within and between plate residuals ranged from −0.05 to + 0.05 and −0.1 to + 0.1, respectively. Total variance was estimated at 0.327; 99.6% of variation could be attributed to the samples. There was no systematic border effect and a negligible correlation between positive internal control and samples results (R2 = 0.213; p < 0.001). Conclusion The results obtained in this study, in highly controlled conditions, point toward the InBios Strongy Detect IgG ELISA assay being reproducible, with no systematic border effect. These results encourage further assay’s development and evaluation for use in practice, including determination of preset cutoff values for positivity, which is currently not provided. Graphical Abstract

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Evaluation of the SsIR/NIE recombinant antigen ELISA for the follow up of patients infected by Strongyloides stercoralis: a diagnostic study

Cited by 3 publications

References 22 publications

Prevalence of Intestinal Parasites, Protozoans and Soil-Transmitted Helminths, in Children from Communities of Northern Argentina after the Interruption of Deworming

Prevalence of Intestinal Parasites, Protozoans and Soil-Transmitted Helminths, in Children from Communities of Northern Argentina after the Interruption of Deworming

Immunodominant Molecules for the Immunodiagnosis of Strongyloides stercoralis Infection

Technical evaluation of the InBios Strongy Detect IgG ELISA assay for the diagnosis of Strongyloides stercoralis infection

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